Wound healing activity of silibinin in mice

Samanta, Rojalini; Pattnaik, Ashok Kumar; Pradhan, Kishanta Kumar; Mehta, Beena K; Pattanayak, Shakti Prasad; Banerjee, Sugato

doi:10.4103/0974-8490.188880

Cited by 26 publications

(9 citation statements)

References 31 publications

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“…During inflammation, the induction of chemokine IL-8 expression requires the cooperative transcriptional activity of both NF-κB and AP-1 factors [39]. According to our results, DHS dose-dependently induced AP-1 activation after prolonged exposure.…”

Section: Discussionsupporting

confidence: 64%

“…NF-κB and AP-1 are transcription factors related to an inflammatory process. These factors are strongly implicated in an initiation of pro-inflammatory target gene expression, e.g., IL-6, IL-8 or COX-2 [13,38,39]. Therefore, we determined the protein levels of the aforementioned factors NF-κB (subunit p65) and AP-1 (subunit c-Jun) by Western blotting using the same experimental conditions (Figure 3a,b,d).…”

Section: Resultsmentioning

confidence: 99%

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Modulation of Skin Inflammatory Response by Active Components of Silymarin

et al. 2018

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In this study, we compared selected silymarin components, such as quercetin (QE), 2,3-dehydrosilybin (DHS) and silybin (SB), with the anti-inflammatory drug indomethacin (IND) in terms of their wound healing potential. In view of the fact that pathological cutaneous wound healing is associated with persistent inflammation, we studied their anti-inflammatory activity against inflammation induced by bacterial lipopolysaccharide (LPS). We investigated the regulation of crucial pro-inflammatory transcription factors—nuclear factor kappa-B (NF-κB) and activator protein 1 (AP-1)—as well as the expression of downstream inflammatory targets by Western blotting, real-time PCR (RT-PCR), electrophoretic mobility shift assay (EMSA), and/or enzyme-linked immunosorbent assay (ELISA) in vitro using primary normal human dermal fibroblasts (NHDF). We demonstrated the greater ability of DHS to modulate the pro-inflammatory cytokines production via the NF-κB and AP-1 signaling pathways when compared to other tested substances. The prolonged exposure of LPS-challenged human dermal fibroblasts to DHS had both beneficial and detrimental consequences. DHS diminished interleukin-6 (IL-6) and interleukin-8 (IL-8) secretion but induced the significant upregulation of IL-8 mRNA associated with NF-κB and AP-1 activation. The observed conflicting results may compromise the main expected benefit, which is the acceleration of the healing of the wound via a diminished inflammation.

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Section: Discussionsupporting

confidence: 64%

Section: Resultsmentioning

confidence: 99%

Modulation of Skin Inflammatory Response by Active Components of Silymarin

et al. 2018

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“…The treatment was given based on the documented procedures. [83][84][85] The mice were anesthetized using subcutaneous injection of ketamine (80 mg/kg) and diazepam (5 mg/kg) on wounding day. The back hair of the animals was removed by shaving.…”

Section: Excision Wound Healing Testmentioning

confidence: 99%

Evaluation of Wound Healing Activity of Methanolic Crude Extract and Solvent Fractions of the Leaves of Vernonia auriculifera Hiern (Asteraceae) in Mice

Lambebo¹,

Kifle²,

Gurji³

et al. 2021

JEP

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Background Medicinal plants play an important role in treating various diseases. Vernonia auriculifera Hiern is one of the medicinal plants used traditionally for the management of wounds. However, there were no scientific reports documented so far on the wound healing activities to substantiate the claim. Thus, the present study provides a scientific evaluation for the wound healing potential of the crude extract as well as solvent fractions of the leaves of Vernonia auriculifera Hiern. Methods Extraction was carried out by maceration using 80% methanol and part of the crude extract fractionated with chloroform, ethyl acetate, and aqueous solvents. Simple ointment bases were prepared using hard paraffin, cetostearyl alcohol, white soft paraffin and wool fat according to British Pharmacopoeia. Then, two types of ointment formulations were prepared from the extract, ie, 5% w/w and 10% w/w. Mice and rats were employed for wound healing study and dermal toxicity test, respectively. Wound healing effects were evaluated by percent of wound contraction, period of epithelialization, tensile strength, and histological analysis as parameters in excision, incision, and burn wound models. Finally, the data were expressed as mean ± SEM, and the results were analyzed using one-way ANOVA followed by post hoc Tukey-test. Results In the excision wound model, the 10% w/w crude extract ointment showed significant wound contraction ( P < 0.001) from day 4 to day 18 as compared to the negative control. Both the 5% w/w ( P < 0.001) and 10% w/w ( P < 0.05) crude extract ointments have showed statistically significant difference in epithelialization period as compared to the negative control. Groups treated with the ethyl acetate and aqueous fractions ointments in incision wound model showed a statistically significant ( P < 0.001) increase in tensile. The 10% w/w and 5% w/w ointments of the crude extract showed a significant ( P < 0.001) increase in breaking strength compared to simple ointment and the untreated control groups. In burn wound model, significant reduction in epithelialization period was observed in 5% w/w ( P < 0.05) and 10% w/w ( P < 0.001), and the percentage of wound contraction was significantly increased in most of post wounding days by 10% w/w ( P < 0.001) and 5% w/w ( P < 0.05) crude extract ointments and compared to the negative control. Conclusion The crude, aqueous, and ethyl acetate fraction of Vernonia auriculifera leaves possess wound healing activities. This finding justifies the use of the leaves of this plant for wound healing as claimed in the traditional medicine literature.

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“…This action was associated with an inhibition of oxidative stress and inflammation ( Jain et al, 2015 ). As shown in another study, the repeated topical application (14 days treatment) of a silibinin-based gel resulted in an efficient wound healing strategy, by acting on tissue re-epithelization, collagen production, and deposition of granulation tissue (as shown in Figure 2 ) ( Samanta et al, 2016 ).…”

Section: Plants From Asteraceae Family As Wound-healing Agentsmentioning

confidence: 84%

Use of Some Asteraceae Plants for the Treatment of Wounds: From Ethnopharmacological Studies to Scientific Evidences

et al. 2018

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Severe wounds result in large lesions and/or loss of function of the affected areas. The treatment of wounds has challenged health professionals due to its complexity, especially in patients with chronic diseases (such as diabetes), and the presence of pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa. Taking this into consideration, the development of new therapies for wound healing requires immediate attention. Ethnopharmacological studies performed in different countries have shown the use of several plants from the Asteraceae family as wound-healing agents. Evidences gained from the traditional medicine have opened new ways for the development of novel and more efficient therapies based on the pharmacological properties of these plants. In this article, we discuss the literature data on the use of Asteraceae plants for the treatment of wounds, based on the ethnopharmacological relevance of each plant. Special attention was given to studies showing the mechanisms of action of Asteraceae-derived compounds and clinical trials. Ageratina pichinchensis (Kunth) R.M. King and H. Rob. and Calendula officinalis L. preparations/compounds were found to show good efficacy when assessed in clinical trials of complicated wounds, including venous leg ulcers and foot ulcers of diabetic patients. The compounds silibinin [from Silybum marianum (L.) Gaertn.] and jaceosidin (from Artemisia princeps Pamp.) were identified as promising compounds for the treatment of wounds. Overall, we suggest that Asteraceae plants represent important sources of compounds that may act as new and efficient healing products.

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Wound healing activity of silibinin in mice

Cited by 26 publications

References 31 publications

Modulation of Skin Inflammatory Response by Active Components of Silymarin

Modulation of Skin Inflammatory Response by Active Components of Silymarin

Evaluation of Wound Healing Activity of Methanolic Crude Extract and Solvent Fractions of the Leaves of Vernonia auriculifera Hiern (Asteraceae) in Mice

Use of Some Asteraceae Plants for the Treatment of Wounds: From Ethnopharmacological Studies to Scientific Evidences

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