Wound Healing Activity of Sheep’s Bladder Extracellular Matrix in Diabetic Rats

Abstract

Diabetic ulcers (DUs) are a chronic, non-healing diabetes complication that leads to high hospital expenses and, in extreme cases, to amputation. Peripheral vascular diseases, diabetic neuropathy, abnormal cellular and cytokine activity are among the major factors that hinder diabetic wound healing. DUs represent an important challenge in the development of new and efficient wound dressings. The extracellular matrix (ECM) has been effectively used as a scaffold for constructive remodeling of multiple tissues in animal and human. Sheep’s urinary bladder matrix was evaluated for its wound healing activity in streptozotocin-induced diabetic rats using excision model. In this experiment, 48 male Sprague dawley rats weighing 220–250[Formula: see text]g were divided into four equal groups of control, vaseline, diabetics + (10[Formula: see text]mg/wound) and [Formula: see text] (50[Formula: see text]mg/wound). Diabetes was induced by intraperitoneal injection of streptozotocin (45[Formula: see text]mg/kg B.W) solved in 0.05[Formula: see text]M citrate buffer. Seven days after confirming diabetes statue, skin wounds were created on the back of each rat. Rate of wound healing and histological assay using hematoxylin and Eosin staining (H&E) were used for evaluation of the wound healing in different groups. ECM treated animals exhibited significant improvement in both wound area and rate of wound healing when compared to controls ([Formula: see text]). The ECM treated wounds were found to epithelize faster as compared to controls. The sheep’s ECM promotes significant wound healing in male diabetic rats and further studies on this activity in animal models and humans are suggested.

Evaluation of the Frequency of Occupational Hazards Among a Group of Iranian General Dentists and its Prevention Criteria

“…Considering the involvement of general scarcity of information in this regard, especially in the dental field in Iran, and in continuing our interest in medical research [24][25][26][27] and most of the published articles on occupational hazards are in nurses and articles related to occupational hazards in dentists are based on skeletal problems and burnout. Therefore, as there are no studies on occupational hazards of dentists in Kerman covering most of occupational hazards and evaluating the awareness of occupational hazards can help identify the most common problems of dentists, this study was conducted to examine the frequency of occupational hazards in general dentists in Kerman (South East of Iran).…”

Correlation Between Clinical and Histopathologic Diagnosis of Oral Potentially Malignant Disorder and Oral Squamous Cell Carcinoma

“…As a result, it increases the life expectancy and quality of life for patients. Therefore, in continuing our interest in the medical research [14][15][16] and given the varied prevalence and type of premalignant and malignant lesions in different societies and the fact that clinical features cannot provide definitive diagnoses for several lesions, the consistency of clinical and histopathologic diagnoses gains significance for definitive, precise, and, particularly, early diagnoses of premalignant lesions as the main goal of treatment. Accordingly, the present study aimed to evaluate the consistency between the clinical and histopathological features of oral potentially malignant disorders and oral squamous cell carcinoma in a 20year period.…”

Functional Antagonism of Sphingosine-1-Phosphate Receptor 1 Prevents Harmaline-Induced Ultrastructural Alterations and Caspase-3 Mediated Apoptosis

“…The tissues were hydrated and stained with Harris hematoxylin and eosin Y. Completely dehydrated sections were cleared with xylene and mounted with entellan (27).…”