Wound healing activity of curcumin conjugated to hyaluronic acid:in vitroandin vivoevaluation

Abstract

Curcumin is a promising wound healing agent but its clinical application is limited due to hydrophobicity and lack of stability. In this article, we report the results of a study on wound healing efficacy of curcumin conjugated to hyaluronic acid (HA) which is a natural polysaccharide known to influence the healing process. Studies on proliferation, antioxidant activity and scratch wound healing carried out in human keratinocyte cells revealed that HA-conjugated curcumin treatment enhanced cell proliferation, decreased oxidative damage induced by HO and also improved migration of cells in scratch wounds as compared to treatment with native curcumin. HA conjugated curcumin exhibited bactericidal activity in dark and phototoxicity when irradiated with blue light against antibiotic resistant bacteria. Furthermore, wound healing efficacy studied in diabetic mice demonstrated that topical application of the conjugate on wounds led to better healing as compared to treatment with HA-free curcumin and HA alone. These results suggest that HA conjugation is a promising formulation of curcumin for enhancing its healing efficacy.

Macrophage repolarization using CD44-targeting hyaluronic acid–polylactide nanoparticles containing curcumin

“…The synthesis of HA-ADH followed the protocol of Park et al [28] and Luo et al [29] and Briefly, ADH (100 mg, 250 lmol) was dissolved in ddH 2 O (20 ml) to prepare HA solution of 5 mg ml À1 . 10 mmol of ADH was added to the HA solution and dissolved completely by stirring for 20 min, followed by adjusting pH to 4.8 using 1.0 N HCl.…”

“…HA-based NPs have presented as promising nano delivery systems owing to the non-toxic, biocompatible, biodegradable and non-immunogenic properties of HA. Importantly, HA has been described to specifically bind to CD44 receptors overexpressed on surface of macrophages [26,27].…”

PLGA microsphere-based composite hydrogel for dual delivery of ciprofloxacin and ginsenoside Rh2 to treat Staphylococcus aureus -induced skin infections

“…prepared. HA was applied to promote moisture retaining, wound healing and tissue regeneration (Sharma et al, 2018). The neutral pH value (7.28 ± 0.18) of TSG pre-gel solution implied its low irritation to human skin.…”

Hyaluronic Acid in the 3^rd Millennium

“…Moreover, HA-curcumin conjugate displays improved healing properties compared to free curcumin and free HA, both in vitro -wound model of human keratinocytes-and in vivo -wound model of diabetic mouse. Hence, HA conjugated curcumin may be useful to treat diabetic wounds [182]. Finally, the most promising and thoroughly studied conjugations of active ingredients to HA involve antitumoral agents, which can be strategically carried to malignant cells by hyaluronan, as explained in the subsection 6.2 [179,183].…”

“…For this reason, HA is used in topical formulations (such as Connettivina® by Fidia) to treat skin irritations and wounds such as abrasions, post-surgical incisions, metabolic and vascular ulcers, and burns [82,[210][211][212][213]. Currently, HA derivatives [173,182,214,215] and HAbased wound dressings, films or hydrogels enriched with other therapeutic agents [216,217] are being evaluated in order to understand if the cicatrisation process could be further enhanced. The wound healing properties of HA and its derivatives are being explored not only in dermatology, but also in other medical fields such as ophthalmology [56,218], otolaryngology [142], rhinology [219] and odontology [220].…”