Wound Healing Activity of 80% Methanol Leaf Extract of Zehneria scabra (L.f) Sond (Cucurbitaceae) in Mice

Tekleyes, Bezu; Huluka, Solomon Assefa; Wondu, Kebede; Wondmkun, Yohannes Tsegyie

doi:10.2147/jep.s303808

Cited by 22 publications

(23 citation statements)

References 24 publications

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“…Under the investigation by Tekleyes et al, the acute cutaneous toxicity test of the 80% methanol extract of Z. scabra was performed according to the OECD 404 guideline. 19 …”

Section: Methodssmentioning

confidence: 99%

In-vivo Wound Healing and Anti-Inflammatory Activity of the Solvent Fraction of Zehneria scabra L.F. Sond (Cucurbitaceae) Leaves

Fisseha¹,

Hammeso²,

Nureye³

et al. 2022

JEP

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Background Wounds continue to be a difficult clinical problem, with early and late consequences causing significant morbidity and death. As a result, proper wound management is critical. In addition to contemporary medicine, medicinal herbs serve an essential role in the treatment of wounds and bacterial infections. Z. scabra is a medicinal plant that has traditionally been used to treat wounds. However, there are no scientific reports on solvent fraction wound healing activities. As a result, the current study presents a scientific assessment of the wound healing ability of the solvent fractions of Z. scabra leaves. Methods The leaves were crushed and macerated three times in 80% methanol. Chloroform, ethyl acetate, and aqueous fractions of simple ointment at 5% w/w and 10 percent w/w strengths were prepared using the fusion technique based on the British Pharmacopoeia. Excision and incision wound models were used to assess the solvent fractions’ wound healing activities. The anti-inflammatory efficacy of crude and solvent fractions was tested in mice utilizing a carrageenan-induced hindpaw edema model. Results In rats, a test dose of 2000 mg/kg of the 10% w/w crude extract ointment was found to be safe. Groups treated with the 5% and 10% ethyl acetate fractions of the extract experienced significant (p<0.05 and p<0.01) wound reduction in the excision wound model. When compared to the negative control, the length of epithelization in groups treated with 10% ethyl acetate fraction and aqueous fractions of Z. scabra was statistically significant (p 0.001). By lowering the amount of carrageenan-induced paw edema, the leaf extract and the chloroform fraction of Z. scabra demonstrated a dose-dependent anti-inflammatory effect. Conclusion The extract showed remarkable wound healing and anti-inflammatory activity and might be recommended for the treatment of many types of human wounds.

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“…Under the investigation by Tekleyes et al, the acute cutaneous toxicity test of the 80% methanol extract of Z. scabra was performed according to the OECD 404 guideline. 19 …”

Section: Methodssmentioning

confidence: 99%

In-vivo Wound Healing and Anti-Inflammatory Activity of the Solvent Fraction of Zehneria scabra L.F. Sond (Cucurbitaceae) Leaves

Fisseha¹,

Hammeso²,

Nureye³

et al. 2022

JEP

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“…After the residual ointment was removed, the mice were observed daily for the development of any adverse skin reaction in terms of edema and erythema for 14 days. 32…”

Section: Acute Dermal Toxicity Testmentioning

confidence: 99%

“…The anti-inflammatory, free radical scavenging activity thus prevents cell damage, enhances cellular vascularity which decreases cell death and antimicrobial activity of flavonoids are important effect that promotes wound healing and shortens the period of epithelialization. 1,32 The wound-healing effect of steroids and phenolic compounds could be due to their antimicrobial and anti-inflammatory activity. 44 The fast wound contraction in excision and burn wound models by the extract could be related to greater collagen fibers, fibroblasts, and capillary blood cells as indicated by histological investigation.…”

mentioning

confidence: 99%

Evaluation of Wound Healing Activity of 80% Methanol Root Crude Extract and Solvent Fractions of Stephania abyssinica (Dill. & A. Rich.) Walp. (Menispermaceae) in Mice

Yiblet¹,

Tsegaw²,

Ahmed³

et al. 2022

JEP

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The root of Stephania abyssinica (Dill. and A. Rich.) Walp. (Menispermaceae) is traditionally used to treat wounds. Despite the fact that there have been in vitro studies and claims supporting wound healing, there has been no scientific data on the in vivo wound healing activities of the root of S. Abyssinica. Objective: The aim of the study was to evaluate the wound healing activity of 80% methanol root extract and solvent fractions of S. Abyssinica in mice. Methods: The roots of S. Abyssinica were air dried, ground and macerated by 80% methanol three times successively. The crude extract was fractionated by water, hexane and ethyl acetate separately. The acute dermal toxicity test was done by applying 2000 mg/ kg of the 10% w/w crude extract. Wound healing activity of crude extract was evaluated on excision, incision and burn wound models, while the fractions were evaluated on excision wound model only. Results: In mice, an acute dermal toxicity test of 2000 mg/kg of the 10% w/w crude extract was found to be safe. The 10% w/w crude extract ointment (CEO) produced significant (p < 0.001) wound contraction from 4th to 16th post wounding days, and the 5% w/w CEO were significant (p < 0.01) wound contraction on 10th post wounding day as compared to simple ointment (SO) treated group on excision wound. On burn wound models, the CEO showed highly significant (p < 0.001) from the 6th post wounding days onwards. The tensile strength was increased significantly (p < 0.001) by the CEO treated mice as compared to the untreated group and SO group. Conclusion:The data obtained from this study showed 80% methanol crude extract, the aqueous and the 10% w/w ethyl acetate fraction possessed better wound healing activities, and decreased period of epithelialization.

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“…Namun ketika suatu kasus dimana perbedaan itu diamati, diketahui bahwa tikus betina sedikit lebih sensitif [32]. Sementara itu, sebanyak 3 penelitian lain menggunakan mencit sebagai hewan ujinya [7,45,46]. Persyaratan hewan uji lainnya menurut OECD No.…”

Section: Uji Toksisitas Akut Dermalunclassified

Toksisitas Akut Dermal Ekstrak Tanaman Sebagai Bagian dalam Aspek Keamanan Obat Herbal Terstandar Topikal

2022

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Inflamasi dermal, misalnya jerawat (acne vulgaris), secara tradisional, sering diobati menggunakan tanaman, sehingga beberapa riset mengeksplorasi ekstrak tanaman secara in vitro. Namun demikian, penggunaan ekstrak tanaman sebagai obat topikal tetap harus mengikuti Peraturan Kepala Badan Pengawas Obat dan Makanan No. 32/2019 tentang PERSYARATAN KEAMANAN DAN MUTU OBAT TRADISIONAL, yang meliputi tiga aspek yaitu keamanan (safety), mutu (quality), dan efektif (efficacy). Lebih lanjut lagi, keamanan obat topikal dipastikan melalui Uji Toksisitas Akut Dermal, seperti tercantum di dalam Peraturan Kepala Badan Pengawas Obat dan Makanan No. 7/2014 tentang PEDOMAN UJI TOKSISITAS NONKLINIK SECARA IN VIVO. Kajian pustaka ini merangkum uji toksisitas akut dermal ekstrak tanaman yang telah dilakukan di berbagai negara. Kajian dilakukan menggunakan kata kunci “Acute”, “Dermal”, “Toxicity”, “Extract” pada basis data PubMed, dan menghasilkan 15 artikel. Hasil kajian pustaka menunjukkan bahwa semua ekstrak tanaman yang diuji di dalam kelimabelas artikel tersebut dinyatakan aman karena tidak menimbulkan gejala toksisitas ataupun kematian pada dosis di atas 2000 mg/kg BB. Dapat disimpulkan bahwa uji toksisitas akut dermal ekstrak tanaman merupakan faktor penting dalam keamanan pengembangan suatu obat herbal terstandar topikal

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Wound Healing Activity of 80% Methanol Leaf Extract of Zehneria scabra (L.f) Sond (Cucurbitaceae) in Mice

Cited by 22 publications

References 24 publications

In-vivo Wound Healing and Anti-Inflammatory Activity of the Solvent Fraction of Zehneria scabra L.F. Sond (Cucurbitaceae) Leaves

In-vivo Wound Healing and Anti-Inflammatory Activity of the Solvent Fraction of Zehneria scabra L.F. Sond (Cucurbitaceae) Leaves

Evaluation of Wound Healing Activity of 80% Methanol Root Crude Extract and Solvent Fractions of Stephania abyssinica (Dill. & A. Rich.) Walp. (Menispermaceae) in Mice

Toksisitas Akut Dermal Ekstrak Tanaman Sebagai Bagian dalam Aspek Keamanan Obat Herbal Terstandar Topikal

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