Wound Fluid from Chronic Leg Ulcers Contains Elevated Levels of Metalloproteinases MMP-2 and MMP-9

Wysocki, Annette B.; Staiano‐Coico, Lisa; Grinnell, Frederick

doi:10.1111/1523-1747.ep12359590

Cited by 662 publications

(502 citation statements)

References 24 publications

Supporting

Mentioning

455

Contrasting

Unclassified

Order By: Relevance

“…The increased activity of MMP-2 in some (not all) macroscopically normal (control) supraspinatus is thought to represent evidence of an underlying pathological process, since histopathological studies have shown that degenerative change is common in cadaver supraspinatus tendons (Chard et al, 1994;Riley et al, 2001). Activity of MMP-2 and matrix degradation has also been reported in chronic ulcers (Wysocki et al, 1993), chronic wounds (Bullen et al, 1995) and atherosclerotic lesions (Li et al, 1996). We have also recently found up-regulation of MMP-2 gene expression in painful Achilles tendinopathy, and this activity was associated with high levels of matrix turnover as determined by pentosidine cross-link analysis (Ireland et al, 2001 and unpublished observations).…”

Section: Discussionmentioning

confidence: 61%

Matrix metalloproteinase activities and their relationship with collagen remodelling in tendon pathology

Riley

Curry

DeGroot³

et al. 2002

Matrix Biology

323

289

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 61%

Matrix metalloproteinase activities and their relationship with collagen remodelling in tendon pathology

Riley

Curry

DeGroot³

et al. 2002

Matrix Biology

323

289

View full text Add to dashboard Cite

“…Figure 2 illustrates the zymography results. Bands representing the forms of pro-MMP-9 and MMP-9, and pro-MMP-2 and MMP-2 were identified with the aid of previous descriptions 9,12 and molecular mass calculations. It was also possible to observe gelatinolytic activity between 100 kDa and 150 kDa (~130 kDa), which has been described in the literature as an MMP complex that probably contains MMP-9.…”

Section: Resultsmentioning

confidence: 99%

“…[7][8][9]14 Among the different MMPs, MMP-9 appears to be a prognostic marker of healing of chronic ulcers. 10 Furthermore, the course of the healing process in chronic wounds is associated with reduced expression of certain MMPs.…”

Section: Discussionmentioning

confidence: 99%

“…Specific tissue expression of MMP-9 has been identified as a prognostic marker of healing of chronic ulcers. 10 A previous study has demonstrated high levels of both MMP-2 and MMP-9 in chronic VUs of the lower limbs, 9 suggesting that these two MMP isoforms play a critical role in healing of venous ulcers. In view of this, these enzymes' expression and activity could serve as markers of the effectiveness and mechanism of action of therapeutic interventions that are intended to accelerate the healing process of chronic ulcers.…”

Section: Introductionmentioning

confidence: 99%

“…This dysregulation can contribute to destruction of connective tissue and the loss of its normal mechanical properties, with the result that wounds become chronic. 7,8 Studies have detected widespread increase in both activity and expression of MMPs in VUs 7,9 and MMPs 2 and 9, which are also known as the gelatinases, have received special attention. Specific tissue expression of MMP-9 has been identified as a prognostic marker of healing of chronic ulcers.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enzymatic activity analysis of MMP-2 and 9 collected by swab from lower limb venous ulcers

et al. 2014

View full text Add to dashboard Cite

Metalloproteinases play a role in repair of venous ulcers of the lower limbs. The great majority of studies of metalloproteinase enzyme activity conducted to date have employed material from biopsies of ulcers. We evaluated the viability of using zymography to measure the enzyme activity of metalloproteinases 2 and 9 in samples of venous ulcer exudate collected on swabs. The method chosen for processing the samples proved viable in terms of its ability to provide adequate protein concentrations for analysis. Using zymography, we observed that the parameters that provided the best results for analysis of gelatinolytic activity were 0.125 to 0.5 mg of total protein content in the gels and enzymatic activation time of 19 hours (at 37 °C). Collection of venous ulcer fluid using swabs proved to be a simple, rapid and effective method for obtaining samples for measurement of gelatinolytic activity with a minimum degree of invasivity.Keywords: venous ulcer; metalloproteinases; gelatinases. ResumoAs metaloproteinases participam do reparo das úlceras venosas de membros inferiores. Até o momento, estudos de atividade enzimática utilizaram, em sua maior parte, biópsia das úlceras. Objetivamos avaliar a viabilidade da mensuração da atividade enzimática de metaloproteinases 2 e 9, extraídas por swab, em amostras de exsudato de úlcera venosa, através de zimografia. O método de processamento da amostra coletada mostrou-se viável, visto que foi possível obter concentração proteica adequada para análise. Através de zimografia, observamos que as quantidades de proteína total das amostras carregadas nos géis entre 0,125 e 0,5 mg, além do tempo de ativação enzimática de 19 horas (a 37 °C), foram parâmetros adequados e de melhor resultado para a análise da atividade gelatinolítica. A coleta através de swab mostrou-se um método simples, rápido e eficaz para a coleta de fluido de úlcera venosa com o objetivo de mensuração da atividade gelatinolítica com grau mínimo de invasividade.

show abstract

Risk Factors Associated With the Failure of a Venous Leg Ulcer to Heal

Margolis

Berlin²,

Strom³

1999

Arch Dermatol

203

196

View full text Add to dashboard Cite

Background: Venous leg ulcers afflict a significant portion of the population. The most popular form of therapy for venous leg ulcers is a compression bandage (eg, Unna boot), a therapy that is frequently unsuccessful.Objective: To describe risk factors associated with the failure of a wound to heal when treated with a limbcompression bandage for 24 weeks.Design: A retrospective cohort study.Setting: Single-center outpatient specialty clinic at an academic medical center.Participants: Two hundred sixty consecutive patients with chronic venous leg ulcers. Main Outcome Measure:The magnitude of the effect of a given risk factor on the probability that a wound will heal within 24 weeks of care.Results: Based on an assessment of leg wounds during initial office visits, we observed that the failure of a wound to heal within 24 weeks was significantly associated with larger wound area, measured in square centimeters (odds ratio [OR], 1.19; 95% confidence interval [CI], 1.11-1.27), duration of the wound in months (OR, 1.09; 95% CI, 1.04-1.16), history of venous ligation or venous stripping (OR, 4.58; 95% CI, 1.84-11.36), history of hip or knee replacement surgery (OR, 3.52; 95% CI, 1.12-11.08), ankle brachial index of less than 0.80 (OR, 3.52; 95% CI, 1.12-11.08), and the presence of fibrin on more than 50% of the wound surface (OR, 3.42; 95% CI,.Conclusions: Several risk factors are associated with the failure of a patient's venous leg ulcer to heal while using limb-compression therapy. It is prudent to consider these factors when referring a patient to a wound care subspecialists or for alternative therapies.

show abstract

Wound Fluid from Chronic Leg Ulcers Contains Elevated Levels of Metalloproteinases MMP-2 and MMP-9

Cited by 662 publications

References 24 publications

Matrix metalloproteinase activities and their relationship with collagen remodelling in tendon pathology

Matrix metalloproteinase activities and their relationship with collagen remodelling in tendon pathology

Enzymatic activity analysis of MMP-2 and 9 collected by swab from lower limb venous ulcers

Risk Factors Associated With the Failure of a Venous Leg Ulcer to Heal

Contact Info

Product

Resources

About