Wound exudate: composition and functions

Cutting, Keith

doi:10.12968/bjcn.2003.8.sup3.11577

Cited by 213 publications

(180 citation statements)

References 15 publications

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“…Chronic wound fluid is comprised of cytokines, chemokines, growth factors, electrolytes and proteolytic enzymes [30,31,32]. Proteolytic enzymes, in particular matrix metalloproteases (MMP) and serine protease human neutrophil elastase (HNE), are responsible for proteolytic degradation of growth factors [19] and extracellular matrix (ECM) proteins.…”

Section: Introductionmentioning

confidence: 99%

Preparation, Characterization and Activity of a Peptide-Cellulosic Aerogel Protease Sensor from Cotton

Edwards

Fontenot

Prevost

et al. 2016

Sensors

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Nanocellulosic aerogels (NA) provide a lightweight biocompatible material with structural properties, like interconnected high porosity and specific surface area, suitable for biosensor design. We report here the preparation, characterization and activity of peptide-nanocellulose aerogels (PepNA) made from unprocessed cotton and designed with protease detection activity. Low-density cellulosic aerogels were prepared from greige cotton by employing calcium thiocyanate octahydrate/lithium chloride as a direct cellulose dissolving medium. Subsequent casting, coagulation, solvent exchange and supercritical carbon dioxide drying afforded homogeneous cellulose II aerogels of fibrous morphology. The cotton-based aerogel had a porosity of 99% largely dominated by mesopores (2–50 nm) and an internal surface of 163 m2·g−1. A fluorescent tripeptide-substrate (succinyl-alanine-proline-alanine-4-amino-7-methyl-coumarin) was tethered to NA by (1) esterification of cellulose C6 surface hydroxyl groups with glycidyl-fluorenylmethyloxycarbonyl (FMOC), (2) deprotection and (3) coupling of the immobilized glycine with the tripeptide. Characterization of the NA and PepNA included techniques, such as elemental analysis, mass spectral analysis, attenuated total reflectance infrared imaging, nitrogen adsorption, scanning electron microscopy and bioactivity studies. The degree of substitution of the peptide analog attached to the anhydroglucose units of PepNA was 0.015. The findings from mass spectral analysis and attenuated total reflectance infrared imaging indicated that the peptide substrate was immobilized on to the surface of the NA. Nitrogen adsorption revealed a high specific surface area and a highly porous system, which supports the open porous structure observed from scanning electron microscopy images. Bioactivity studies of PepNA revealed a detection sensitivity of 0.13 units/milliliter for human neutrophil elastase, a diagnostic biomarker for inflammatory diseases. The physical properties of the aerogel are suitable for interfacing with an intelligent protease sequestrant wound dressing.

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Section: Introductionmentioning

confidence: 99%

Preparation, Characterization and Activity of a Peptide-Cellulosic Aerogel Protease Sensor from Cotton

Edwards

Fontenot

Prevost

et al. 2016

Sensors

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“…Within a wound, slough (largely composed of denatured proteins 37 ), necrotic tissue, and even nonbiological components such as fibers from a dressing, are all surfaces. In the mucus or sputum of cystic fibrosis patients biofilms are referred to as microbial aggregates and are dispersed within the mucus itself.…”

Section: Identification Of a Biofilmmentioning

confidence: 99%

Biofilms and Wounds: An Identification Algorithm and Potential Treatment Options

Percival

Vuotto

Donelli

et al. 2015

Advances in Wound Care

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“…A moist wound environment is mainly sustained by exudate. The exudate mostly consists of water, but it also contains electrolytes, nutrients, proteins, inflammatory mediators, proteases such as matrix metalloproteinases (MMPs), growth factors and waste products, as well as white blood cells such as neutrophils, macrophages and platelets [40,42,43]. The wound exudate is produced by vasodilation during the early inflammatory stage of healing under the influence of elevated levels of inflammatory mediators and activators such as histamine and bradykinin [13,[44][45][46].…”

Section: Absorbent Wound Dressingsmentioning

confidence: 99%

A Review on Antimicrobial Silver Absorbent Wound Dressings Applied to Exuding Wounds

Yang¹,

Hu²

2015

J Microb Biochem Technol

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Exuding often occurs in the inflammatory stage both for acute wounds and chronic wounds. Absorbent wound dressings are specially developed and used in exuding wounds. In order to reduce bacteria and risk of infection, many absorbent dressings are impregnated with silver ions or particles which are the most widely used substance to obtain antimicrobial effects and therefore this review focuses on the antimicrobial absorbent dressings treated with silver ions or silver particles. The methods of dispersing silver in dressings include coating or spraying silver-containing solution on the wound dressing surface, padding the wetted dressing with pressure, embedding silver nanoparticles in non-woven fibers. When silver dressings absorb exudate from wounds, the antimicrobial silver will be activated and then breaks down the bacterial cell wall to kill the bacterium. Silver foam dressings and silver alginate dressings are the most popular antimicrobial absorbent dressings, which have been developed and produced by many health care companies.

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Wound exudate: composition and functions

Cited by 213 publications

References 15 publications

Preparation, Characterization and Activity of a Peptide-Cellulosic Aerogel Protease Sensor from Cotton

Preparation, Characterization and Activity of a Peptide-Cellulosic Aerogel Protease Sensor from Cotton

Biofilms and Wounds: An Identification Algorithm and Potential Treatment Options

A Review on Antimicrobial Silver Absorbent Wound Dressings Applied to Exuding Wounds

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