Wound age estimation based on chronological changes in chitinase 3-like protein 1 expression

Murase, Takayuki; Shinba, Yoriko; Mitsuma, Masahide; Abe, Yuki; Yamashita, Hiromi; Ikematsu, Kazuya

doi:10.1016/j.legalmed.2022.102128

Cited by 2 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In human cadaver skin wounds, weak CHI3L1 expression (0.11 ± 0.024) was observed on days 0 to 1. From day 4 to 6, there was a significant increase in CHI3L1-expressing cells (5.35 ± 0.35), followed by a decrease in expression from day 7 to 13 (1.53 ± 0.24) [72].…”

Section: Other Markersmentioning

confidence: 96%

Dating Skin Lesions of Forensic Interest by Immunohistochemistry and Immunofluorescence Techniques: A Scoping Literature Review

Tomassini,

Lancia,

Scendoni

et al. 2024

Diagnostics

View full text Add to dashboard Cite

Wound age estimation is a significant issue in forensic pathology. Although various methods have been evaluated, no gold standard system or model has been proposed, and accurate injury time estimation is still challenging. The distinction between vital skin wounds—i.e., ante-mortem lesions—and skin alterations that occur after death is a crucial goal in forensic pathology. Once the vitality of the wound has been confirmed, the assessment of the post-trauma interval (PTI) is also fundamental in establishing the causal relationship between the traumatic event and death. The most frequently used techniques in research studies are biochemistry, molecular biology, and immunohistochemistry (IHC). Biochemical methods take advantage of the chemical and physical techniques. A systematic literature search of studies started on 18 February 2023. The search was conducted in the main databases for biomedical literature, i.e., PubMed and Scopus, for papers published between 1973 and 2022, focusing on different techniques of immunohistochemistry and immunofluorescence (IF) for estimating the PTI of skin wounds. The present study involves a comprehensive and structured analysis of the existing literature to provide a detailed and comprehensive overview of the different IHC techniques used to date skin lesions, synthesize the available evidence, critically evaluate the methodologies, and eventually draw meaningful conclusions about the reliability and effectiveness of the different markers that have been discovered and used in wound age estimation.

show abstract

Section: Other Markersmentioning

confidence: 96%

Dating Skin Lesions of Forensic Interest by Immunohistochemistry and Immunofluorescence Techniques: A Scoping Literature Review

Tomassini,

Lancia,

Scendoni

et al. 2024

Diagnostics

View full text Add to dashboard Cite

show abstract

“…МикроРНК участвуют в дифференцировке, миграции и апоптозе кератиноцитов [15]. Исследование экспрессии хитиназа-3-подобного белка 1 (CHI3L1) показало, что раны с четвёртого по шестой день после травмы можно чётко отличить от других ран на основе значения отсечения 2,75, чувствительности 92,31% и специфичности 85,14% [50].…”

Section: методы исследования травматических кровоизлиянийunclassified

Use of various research methods in forensic practice to determine lifetime and age of injury: A review

Frolova,

Zabozlaev,

Klevno

2023

Russian Journal of Forensic Medicine

View full text Add to dashboard Cite

The problem of the prescription of damage formation has been of interest to forensic physicians since the XIX century to the present. After analyzing the literature sources on the problem of the prescription of traumatic hemorrhages, it becomes clear that there is no single position, approach to the study of this issue, an unambiguous time frame that accurately determines the period from injury to the moment of death of the victim. Both in the nineteenth century and in the twenty-first, forensic doctors initially focused on the macroscopic picture of the injury followed by microscopic examination. When studying micropreparations, they are guided by: the cellular composition of hemorrhage, based on already known data, on their own experience and knowledge in this matter, on the brief circumstances of the incident (if any are indicated in the direction of forensic histological examination), on the age of the victim. Over the years, with the acquired experience and accumulated knowledge, researchers have determined approximate, developed approximate time frames for the formation of damage, now taking into account not only the above parameters, but also the condition of the victim during and after the injury. But scientists have not come to a consensus. Medicine, achievements, progress and perseverance of scientists strive forward, involving related sciences in the study of antiquity. As a result, such methods as: molecular genetic, biophysical, biochemical, immunohistochemical, etc., have found their application, allowing to study not only the cellular composition, but also enzymatic, hormonal changes in damaged tissues. Instrumental methods are being developed: ultrasound. When using both laboratory and instrumental methods, as well as their combination, this problem remains completely unresolved.

show abstract

Wound age estimation based on chronological changes in chitinase 3-like protein 1 expression

Cited by 2 publications

References 30 publications

Dating Skin Lesions of Forensic Interest by Immunohistochemistry and Immunofluorescence Techniques: A Scoping Literature Review

Dating Skin Lesions of Forensic Interest by Immunohistochemistry and Immunofluorescence Techniques: A Scoping Literature Review

Use of various research methods in forensic practice to determine lifetime and age of injury: A review

Contact Info

Product

Resources

About