Worldwide Diversity ofKlebsiella pneumoniaeThat Produce β-LactamaseblaKPC-2Gene1

Cuzon, Gaëlle; Naas, Thierry; Truong, H. Tuan; Villegas, Maria-Virginia; Wisell, Karin Tegmark; Carmeli, Yehuda; Gales, Ana Cristina; Navon-Venezia, Shiri; Quinn, John P.; Nordmann, Patrice

doi:10.3201/eid1609.091389

Cited by 290 publications

(271 citation statements)

References 38 publications

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“…30 In contrast, it is important to note that currently there is a worldwide spread of ESBL and KPC-producing K. pneumoniae, which seems to occur due to the dissemination of specific clones ST258, ST11 and ST437 in Brazil. 31,32 …”

Section: Resultsmentioning

confidence: 99%

Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Chagas¹,

Alves²,

Vallim³

et al. 2011

Braz J Infect Dis

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Objective: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella pneumoniae collected from hospitals in different cities of Brazil. Material and Methods: Eighty-five K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test and the Etest®. The MIC 50 and MIC 90 for ESBL-producing isolates were determined by the Etest® method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion method according to the CLSI. Screening for bla TEM, bla SHV, bla CTX-M genes and class 1 integron was performed by PCR amplification. To determine the genomic diversity of CTX-M-producers, isolates were analyzed by macrorestriction profile analysis following PFGE. Results and Discussion: Seventy-one K. pneumoniae isolates were ESBL-producing. PCR and sequencing experiments detected 38 CTX-M-producing K. pneumoniae belonged to groups CTX-M 1, CTX-M 2, CTX-M 8 and CTX-M 9. The association of different types ESBL (CTX-M, SHV and TEM) was frequent. All K. pneumoniae isolates carried class 1 integron. PFGE analysis revealed thirty-one clonal types among CTX-M-producing isolates. The data presented herein illustrate the diversity of genotypes of CTX-M producing K. pneumoniae among Brazilians hospitals.

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Section: Resultsmentioning

confidence: 99%

Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Chagas¹,

Alves²,

Vallim³

et al. 2011

Braz J Infect Dis

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“…Durante la última década, se ha reportado a nivel mundial un aumento sostenido en el porcentaje de bacterias Gram negativas que son resistentes a todas las familias de antibióticos y especialmente a los carbapenem (1,4,5). Para que una bacteria Gram negativa exprese un fenotipo resistente a los carbapenem, requiere de la combinación de, al menos, dos mecanismos de resistencia, siendo la más frecuente la producción de enzimas y el cierre de porinas, lo cual, además, le confiere a la bacteria resistencia a otros betalactámicos (6)(7)(8).…”

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Prevalencia de bacterias Gram negativas portadoras del gen blaKPC en hospitales de Colombia

et al. 2013

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34(Supl.1):81-90 Prevalencia de bacterias portadoras de blaKPC en Colombia Contribución de los autores:Robinson Pacheco: realización del estudio, recolección de la información clínica y epidemiológica, realización de los análisis estadísticos y redacción del manuscrito. Lyda Osorio: participación en el diseño del estudio, supervisión de la recolección de información y del análisis estadístico. Adriana M. Correa: coordinación de las actividades de laboratorio, desarrollo de la técnica molecular de PFGE. María Virginia Villegas: participación en el diseño y coordinación general del estudio. Todos los autores participaron en la revisión y corrección del manuscrito. ARTÍCULO ORIGINAL Biomédica 2014;34(Supl.

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“…The most common carbapenemases in K. pneumoniae are the K. pneumoniae carbapenemases (KPC) [6]. KPC-associated infections are predominantly nosocomial, involving patients with multiple risk factors, and the mortality rates can be high [7].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from a university hospital in Brazil

Vivan¹,

Rosa²,

Rizek³

et al. 2017

J Infect Dev Ctries

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Introduction: The emergence of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-Kpn) isolates is attracting significant attention in nosocomial infection settings. K. pneumoniae is the main pathogen that harbours blaKPC genes. Methodology: This study evaluated 54 K. pneumoniae carbapenem-resistant isolates from patients hospitalized at the University Hospital of Londrina, between July 2009 and July 2010. The isolates were phenotypically screened for carbapenemase production and submitted for genotypic confirmation by polymerase chain reaction (PCR) for KPC, metallo-β-lactamases, OXA-48, and extended-spectrum beta-lactamase genes. The absence of outer membrane proteins (OMP) was investigated by SDS-PAGE. The susceptibility profile was determined by broth microdilution, according to Clinical and Laboratory Standards Institute protocol. Results: All isolates were phenotypically positive for class A carbapenemase production, but negative for metallo-β-lactamase activity. PCR analysis demonstrated that all isolates carried blaKPC genes and sequencing showed that all strains belonged to KPC-2 subtype. Four strains did not show porin expression, and all isolates were resistant to ertapenem, meropenem, and imipenem. Susceptibility rates reached 35.2% for gentamicin, 85.2% for polymixyn B, 87% for colistin, and 98.1% for both tigecycline and fosfomycin. Pulsed-field gel electrophoresis showed six clones, and three of them predominated among the isolates. Conclusions: KPC-2-producing K. pneumoniae is becoming predominant among carbapenem-resistant K. pneumoniae isolates at the hospital. The association of the enzyme KPC with other resistance determinants, such as loss of porins, may increase the severity of the situation of nosocomial infections. There is an urgent need to develop strategies for infection control and prevention.

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Worldwide Diversity ofKlebsiella pneumoniaeThat Produce β-Lactamasebla_KPC-2Gene1

Abstract: TOC summary: Clones harboring different plasmids with identical genetic structure could be the origin of worldwide spread.

Cited by 290 publications

References 38 publications

Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Prevalencia de bacterias Gram negativas portadoras del gen blaKPC en hospitales de Colombia

Molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from a university hospital in Brazil

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