World human neutrophil antigens investigation survey

Abstract

Background and ObjectivesHuman neutrophil antigens (HNAs) are categorized into five systems: HNA‐1 to HNA‐5. Given the importance of neutrophils in immunity, we sought to create awareness of the role of HNA diagnostic services in managing immune neutropenia and transfusion‐related acute lung injury. To provide health communities all around the world with access to these services, we conducted a survey to create a directory of these HNA diagnostic services.Materials and MethodsAn Excel table‐based survey was created to capture information on the laboratory's location and was emailed to 55 individuals with known or possible HNA investigation activity. The collected data were then summarized and analysed.ResultsOf contacted laboratories, the surveys were returned from 23 (38.2%) laboratories; 17 have already established HNA diagnostic (of them 12 were regular participants of the International Granulocyte Immunobiology Workshop [ISBT‐IGIW]), 4 laboratories were in the process of establishing their HNA investigation and the remaining 2 responder laboratories, did not conduct HNA investigations. In established laboratories, investigation for autoimmune neutropenia (infancies and adults) was the most frequently requested, and antibodies against HNA‐1a and HNA‐1b were the most commonly detected.ConclusionThe directory of survey respondents provides a resource for health professionals wanting to access HNA diagnostic services. The present study offers a comprehensive picture of HNA diagnostics (typing and serology), identifying weak points and areas for improvement for the first time. Identifying more laboratories involved in HNA diagnostics with limited access to international societies in the field will globally improve HNA diagnostics.

A novel immunocomplex capture fluorescence assay (ICFA) using fluorescent beads and transfected cells for specific identification of human neutrophil antigen (HNA)‐1a and ‐1b antibodies

“…It may have been better to use all 5 mAbs for this comparison. Nonetheless, 3G8 and LNK16 are commonly used for MAIGA assays 21 . Therefore, we also assessed the nine samples with known specificity using the MAIGA assay with 3G8.…”

Primary autoimmune neutropenia in early childhood - Genetic variations and antibody specificity

“…Multi and monoclonal antibody immobilization of granulocyte antigen (MAIGA), each of which have different advantages and disadvantages [85]- [87]. The combination of GIFT, GAT and MAIGA assays is considered the 'gold standard' [87].…”

“…It is recommended that more than one technique be used to detect HNA-specific antibodies. Together with serological antibody testing, genotyping of HNA-1, HNA-3, HNA-4 and HNA-5 are typically performed [87]; only a few laboratories test for HNA-2 [87].…”