Background: Workplace Bullying (WPB) is any form of verbal, physical or sexual harassment that an employee endures by a person or a group. In health care settings, practitioners are occasionally victims of WPB. The aim of this study was to evaluate the various characteristics of WPB incidents at a multi-regional healthcare facility in a Middle Eastern country. Methods: A cross sectional study survey was conducted in 2018, distributing a self-administrated questionnaire via private electronic mails among fulltime healthcare practitioners in multi-regional hospitals. Eligible participants were 684 practitioners who have been exposed to a WPB incident in the past five years. Factors associated with either being a victim or perpetrators were analyzed, including their age, sex, marital status, level of education, nationality, years of experience, and job title Data were analyzed using SPSS statistical software version 25, applying both descriptive and inferential statistical analysis as appropriate. Results: Majority of participants were females (89.2%), and 39.3% were <30 years old. Practitioners were nurses (49.1%), administrative staff (24.3%), physicians (14.6%), technicians (10.8%) and pharmacists (1.2%). WPB perpetrators were mainly patients (36.1%), their families/relatives (29.5%), other colleagues (27.2%) or their managers/supervisors (7.2%). The type of WPB incident was mainly a verbal abuse (98.1%), a physical harassment (11.8%) and sexual connotations (5.8%). WPB mainly occurred during day duties (80.3%) and at working stations/offices (52.6%). Higher-risk groups among nurses/allied health were mainly females, ≥30 years old, and expatriates. Among physicians and pharmacists, males, younger age groups, and locals were more prone to WPB. Nurses/allied heath were more likely to encounter WPB in offices/non-clinic areas, whereas physicians/pharmacists encountered them in patient rooms/clinics. Older practitioners (≥30 years) were mainly exposed to WPB by perpetrators who were employees, while younger practitioners encountered WPB by patients/relatives. Perpetrators who were employees committed WPB in offices/non-clinic areas, while patients/their relatives committed WPB in patient rooms/clinics. Conclusion: WPB might occur at anytime or anywhere within hospitals and by any person. This study shared the characteristics of WPB incidents that did occur in one setting and might occur in any other setting too. Analyzing the victim’s, the perpetrator’s and the setting’s characteristics provides a clear picture on the early warning signs that might predict a high-risk environment and alert hospital safety officers and hospital administration.