Workflow to Characterize Mutants with Reproductive Defects

Noble, Jennifer; Palanivelu, Ravishankar

doi:10.1007/978-1-0716-0672-8_8

Cited by 4 publications

(2 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pollinated pistils were incubated in the control or heat stress conditions for 12 hours. Pistils were excised and an aniline blue assay of pollinated pistils was performed as described 116 . Stained pistils were mounted and observed using epifluorescence microscopy on a Zeiss Axiovert 100 fluorescent microscope.…”

Section: Tomato Plant Growthmentioning

confidence: 99%

Enhanced pollen tube performance at high temperature contributes to thermotolerant fruit production in tomato

Yimga Ouonkap,

Palaniappan,

Pryze

et al. 2024

Preprint

View full text Add to dashboard Cite

Rising temperature extremes during critical reproductive periods threaten the yield of major grain and fruit crops. Flowering plant reproduction depends on development of sufficient numbers of pollen grains and on their ability to generate a cellular extension, the pollen tube, which elongates through the pistil to deliver sperm cells to female gametes for double fertilization. These critical phases of the life cycle are sensitive to temperature and limit productivity under high temperature (HT). Previous studies have investigated the effects of HT on pollen development, but little is known about how HT applied during the pollen tube growth phase affects fertility. Here, we used tomato as a model fruit crop to determine how HT affects the pollen tube growth phase, taking advantage of cultivars noted for fruit production in exceptionally hot growing seasons. We found that exposure to HT solely during the pollen tube growth phase limits fruit biomass and seed set more significantly in thermosensitive cultivars than in thermotolerant cultivars. Importantly, we found that pollen tubes from the thermotolerant Tamaulipas cultivar have enhanced growthin vivoandin vitrounder HT. Analysis of the pollen tube transcriptome’s response to HT allowed us to develop hypotheses for the molecular basis of cellular thermotolerance in the pollen tube and we define two response modes (enhanced induction of stress responses, and higher basal levels of growth pathways repressed by heat stress) associated with reproductive thermotolerance. Importantly, we define key components of the pollen tube stress response identifying enhanced ROS homeostasis and pollen tube callose synthesis and deposition as important components of reproductive thermotolerance in Tamaulipas. Our work identifies the pollen tube growth phase as a viable target to enhance reproductive thermotolerance and delineates key pathways that are altered in crop varieties capable of fruiting under HT conditions.Abstract Figure

show abstract

Section: Tomato Plant Growthmentioning

confidence: 99%

Enhanced pollen tube performance at high temperature contributes to thermotolerant fruit production in tomato

Yimga Ouonkap,

Palaniappan,

Pryze

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Investigating pollen tube growth through the pistil tissue would be an ideal way to study physical barriers associated with this process. Although various methodologies are used to realize this goal [27,28], this scenario imposes several critical limitations, including (1) asynchronous germination of pollen tubes, (2) crowding of pollen tubes within the pistil, (3) lack of control on the complex pistil biological system, and (4) the requirement for advanced imaging methods to visualize pollen tubes in living pistils. To circumvent some of these challenges, microfluidic platforms have been exploited to provide defined microenvironments for single-cell analysis of pollen tubes and address key biophysical questions about their behavior during the process of pistil penetration.…”

Section: Pollen Tube Behavior During Encounters With Inanimate Barriersmentioning

confidence: 99%

Signaling at Physical Barriers during Pollen–Pistil Interactions

Robichaux

Wallace

2021

IJMS

View full text Add to dashboard Cite

In angiosperms, double fertilization requires pollen tubes to transport non-motile sperm to distant egg cells housed in a specialized female structure known as the pistil, mediating the ultimate fusion between male and female gametes. During this journey, the pollen tube encounters numerous physical barriers that must be mechanically circumvented, including the penetration of the stigmatic papillae, style, transmitting tract, and synergid cells as well as the ultimate fusion of sperm cells to the egg or central cell. Additionally, the pollen tube must maintain structural integrity in these compact environments, while responding to positional guidance cues that lead the pollen tube to its destination. Here, we discuss the nature of these physical barriers as well as efforts to genetically and cellularly identify the factors that allow pollen tubes to successfully, specifically, and quickly circumnavigate them.

show abstract

Evolutionary analysis of theLORELEIgene family in plants reveals regulatory subfunctionalization

et al. 2022

View full text Add to dashboard Cite

A signaling complex comprising members of the LORELEI (LRE)-LIKE GPI-anchored protein (LLG) and Catharanthus roseus RECEPTOR-LIKE KINASE 1-LIKE (CrRLK1L) families perceive RAPID ALKALINIZATION FACTOR (RALF) peptides and regulate growth, reproduction, immunity, and stress responses in Arabidopsis (Arabidopsis thaliana). Genes encoding these proteins are members of multi-gene families in most angiosperms and could generate thousands of signaling complex variants. However, the links between expansion of these gene families and the functional diversification of this critical signaling complex as well as the evolutionary factors underlying the maintenance of gene duplicates remain unknown. Here, we investigated LLG gene family evolution by sampling land plant genomes and explored the function and expression of angiosperm LLGs. We found that LLG diversity within major land plant lineages is primarily due to lineage specific duplication events, and that these duplications occurred both early in the history of these lineages and more recently. Our complementation and expression analyses showed that expression divergence (i.e., regulatory subfunctionalization), rather than functional divergence, explains the retention of LLG paralogs. Interestingly, all but one monocot and all eudicot species examined had an LLG copy with preferential expression in male reproductive tissues, while the other duplicate copies showed highest levels of expression in female or vegetative tissues. The single LLG copy in Amborella trichopoda is expressed vastly higher in male compared to in female reproductive or vegetative tissues. We propose that expression divergence plays an important role in retention of LLG duplicates in angiosperms.

show abstract

Workflow to Characterize Mutants with Reproductive Defects

Cited by 4 publications

References 21 publications

Enhanced pollen tube performance at high temperature contributes to thermotolerant fruit production in tomato

Enhanced pollen tube performance at high temperature contributes to thermotolerant fruit production in tomato

Signaling at Physical Barriers during Pollen–Pistil Interactions

Evolutionary analysis of theLORELEIgene family in plants reveals regulatory subfunctionalization

Contact Info

Product

Resources

About