Work horse strainClostridioides difficile630Δermis oblivious to its anaerobic lifestyle

Abstract

The laboratory reference strain 630erm of the anaerobic human pathogen Clostridioides difficile is characterized by a remarkable high oxygen tolerance. We show that an amino acid exchange in the DNA binding domain of the hydrogen peroxide sensor PerR results in a constitutive derepression of PerR-controlled genes and thus in an oxidative stress response even under anaerobic conditions. This questions the model status, strain 630erm claims in C. difficile research. Main TextLegend of Dataset S1 Dataset S1 Sequence alignment of Fur family proteins of different bacteria Sequences of over 900 proteins of the Fur family were aligned using a PSI-Blast via Phyre2 (13). Conserved amino acid residues are indicated at the end of the table.