Wolbachia strains in cryptic species of the Anastrepha fraterculus complex (Diptera, Tephritidae) along the Neotropical Region

Prezotto, Leandro F; Perondini, André Luiz Paranhos; Hernández‐Ortiz, Vicente; Marino, Celso Luís; Selivon, Denise

doi:10.1016/j.syapm.2016.11.002

Cited by 12 publications

(17 citation statements)

References 60 publications

(116 reference statements)

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“…These patterns suggest the existence of one or several Wolbachia strains harboured by different A. obliqua populations. Similar post‐zygotic isolation patterns have been found between cryptic species in the A. fraterculus complex (Devescovi et al., ), which harbour different strains of Wolbachia (Prezotto, Perondini, Hernández‐Ortiz, Marino, & Selivon, ; Selivon et al., , ). Exploring the existence of the bacteria and characterizing its different strains among A. obliqua populations are important since it could facilitate colonization, strain refreshment, efficient mass rearing for application of the SIT, and eventually lead to the development of the incompatible insect technique (IIT) (Zhang, Lees, Xi, Bourtzis, & Gilles, ).…”

Section: Discussionsupporting

confidence: 63%

Reproductive compatibility among Mexican populations of Anastrepha obliqua: theoretical and management implications

Rull

Tadeo

Lasa

et al. 2018

J Applied Entomology

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The fraterculus species group, composed of 34 species in the genus Anastrepha (Diptera: Tephritidae), includes the fraterculus cryptic species complex formed by eight reproductively isolated morphotypes. A previous study revealed six genetic mitochondrial types of Anastrepha obliqua, suggesting the existence of a second cryptic species complex. However, marked discrepancies between nuclear and mitochondrial loci rather suggest incomplete lineage sorting or introgression between A. obliqua and A. fraterculus. Such hybridization could nevertheless result in reproductive isolation, an outcome that could affect efficacy of area‐wide management for the most important pest of mangos in America. Two mitochondrial types occur in Mexico, and the limits of a third one, encompassing Central American populations, have not been clearly established. Here, we tested reproductive compatibility among three A. obliqua populations from the Pacific and a population from the Gulf of Mexico. No evidence of pre‐zygotic isolation was found. Flies from the Atlantic mated randomly for equal duration with individuals from three Pacific populations. Homotypic and heterotypic crosses produced similar numbers of eggs, with heterotypic crosses of Pacific males and Atlantic females hatching in lower proportions. Larvae of all cross types developed equally in mangos and exhibited no sex ratio distortion of hybrid F1. The three mitochondrial types identified in Mexico and Central America do not appear to be cryptic species and can be managed using one single strain for the sterile insect technique.

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Section: Discussionsupporting

confidence: 63%

Reproductive compatibility among Mexican populations of Anastrepha obliqua: theoretical and management implications

Rull

Tadeo

Lasa

et al. 2018

J Applied Entomology

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“…The presence of the same wsp gene sequence in different populations of A. fraterculus does not necessarily mean that they are infected with identical Wolbachia strains [16,119]. The Wolbachia infection status of several morphotypes of the A. fraterculus cryptic species complex (including A. fraterculus sp.1) was recently published by Prezotto et al [93]. The information provided by these authors with respect to Wolbachia sequence variants infecting different A. fraterculus populations from Argentina (either using MLST or wsp HVR analyses) differs from our findings.…”

Section: Discussionmentioning

confidence: 99%

“…The information provided by these authors with respect to Wolbachia sequence variants infecting different A. fraterculus populations from Argentina (either using MLST or wsp HVR analyses) differs from our findings. More knowledge regarding the origin of the samples and the number of individuals analyzed by Prezotto et al [93] are needed in order to compare the results obtained in the two studies. Moreover, the same authors suggested a potential association between specific Wolbachia strains and distinct A. fraterculus morphotypes, which could act as a reinforcing factor in the diversification processes, providing also, some evidence of the possible way of transmission of Our crossing experiments showed a detrimental effect during the development for crosses involving Af-Cast-1 females.…”

Section: Discussionmentioning

confidence: 99%

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Wolbachiainfection in Argentinean populations ofAnastrepha fraterculus sp1: preliminary evidence of sex ratio distortion by one of two strains

Conte

Segura

Milla

et al. 2019

Preprint

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Background: Wolbachia, one of the most abundant taxa of intracellular Alphaproteobacteria, is widespread among arthropods and filarial nematodes. The presence of these maternally inherited bacteria is associated with modifications of host fitness, including a variety of reproductive abnormalities, such as cytoplasmic incompatibility, thelytokous parthenogenesis, host feminization and male-killing. Wolbachia has attracted much interest for its role in biological, ecological and evolutionary processes as well as for its potential use in novel and environmentally-friendly strategies for the control of insect pests and disease vectors including a major agricultural pest, the South American fruit fly, Anastrepha fraterculus Wiedemann (Diptera: Tephritidae). Results:We used wsp, 16S rRNA and a multilocus sequence typing (MLST) scheme including gatB, coxA, hcpA, fbpA, and ftsZ genes to detect and characterize the Wolbachia infection in laboratory strains and wild populations of A. fraterculus from Argentina. Wolbachia was found in all A. fraterculus individuals studied. Nucleotide sequences analysis of wsp gene allowed the identification of two Wolbachia nucleotide variants (named wAfraCast1_A and wAfraCast2_A). After the analysis of 76 individuals, a high prevalence of the wAfraCast2_A variant was found both, in laboratory (82%) and wild populations (95%). MLST analysis identified both Wolbachia genetic variants as sequence type 13. Phylogenetic analysis of concatenated MLST datasets clustered wAfraCast1/2_A in the supergroup A. Paired-crossing experiments among single infected laboratory strains showed a phenotype specifically associated to wAfraCast1_A that includes slight detrimental effects on larval survival, a female-biased sex ratio; suggesting the induction of male-killing phenomena, and a decreased proportion of females producing descendants that appears attributable to the lack of sperm in their spermathecae. Conclusions:We detected and characterized at the molecular level two wsp gene sequence variants of Wolbachia both in laboratory and wild populations of A. fraterculus sp.1 from Argentina. Crossing experiments on singly-infected A. fraterculus strains showed evidence of a male killing-like mechanism potentially associated to the wAfraCast1_A -A. fraterculus interactions. Further mating experiments including antibiotic treatments and the analysis of early and late immature stages of descendants will contribute to our understanding of the phenotypes elicited by the Wolbachia variant wAfraCast1_A in A. fraterculus sp.1.

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“…Based mostly on PCR and sequencing approaches, ˜66% of ˜86 tephritid species screened have at least one record of positive Wolbachia infection (excluding pseudogenes) in laboratory and natural populations (Table S1; only supergroups A and B have been found in tephritids). For the genus Anastrepha , all but one species ( A. ludens ) of 17 screened to date harbor Wolbachia [142, 144–148, 149; and this study, 150]. Most Anastrepha species harbor Wolbachia strains assigned to supergroup A. Anastrepha striata Schiner and Anastrepha serpentina Wiedemann, however, harbor supergroup B in southern Mexico [147; and this study] and supergroup A in Brazil [146].…”

Section: Wolbachia In Tephritidsmentioning

confidence: 93%

Wolbachia pipientisassociated to tephritid fruit fly pests: from basic research to applications

Mateos

Martínez

Lanzavecchia

et al. 2018

Preprint

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23hominivorax Coquerel); several species of tsetse fly (Glossina spp.); and the codling 71 moth (Cydia pomonella L.) [reviewed in 17, 18]. 72 73 Successful SIT programs as part of Area-wide Integrated Pest Management (IPM) 74 strategies have also been implemented for several tephritids: Ceratitis capitata 75 Wiedemann; Anastrepha ludens Loew; Anastrepha obliqua Macquart; Anastrepha 76 fraterculus Wiedemann; Zeugodacus cucurbitae Coquillett; Bactrocera dorsalis Hendel; 77and Bactrocera tryoni Froggatt [6, 12, 13, 15]. SIT is currently being developed for two 78 additional tephritid species: Dacus ciliatus Loew and Bactrocera tau Walker [19, 20]. 79The advantages of the SIT over other pest control approaches (e.g. use of pesticides) 80 are that it is the most environmentally friendly and resistance is unlikely to evolve [21, 81 22]. 82 83 Another autocidal strategy where mating between mass-reared and wild insects can be 84 used to suppress pest populations is the incompatible insect technique (IIT). IIT also 85 relies on the principle of reducing female fertility, but utilizes endosymbiotic bacteria 86 instead of radiation, to induce a context-dependent sterility in wild females. It is based 87 on the ability of certain maternally inherited bacteria (namely from the genus Wolbachia) 88to induce a form of reproductive incompatibility known as cytoplasmic incompatibility (CI; 89 explained in the section below). Herein we review the current knowledge on taxonomic 90 diversity of Wolbachia-tephritid associations and their phenotypic consequences, and 91 identify gaps in knowledge and approaches in the context of potential application of IIT in 92 AW-IPM programs to control tephritid pests. We also discuss scenarios where these 93 two autocidal strategies, SIT and IIT, could be potentially combined for the population 94 suppression of tephritid pests. 95 96 5 The influence of Wolbachia on host ecology 97Insects and other arthropods are common hosts of maternally inherited bacteria 98 [reviewed in 23]. These heritable endosymbionts can have a strong influence on host 99 ecology. Such vertically transmitted bacteria are typically vastly (or fully) dependent on 100 the host for survival and transmission. Certain associations are obligate for both 101 partners, and generally involve a nutritional benefit to the host. Other heritable bacteria 102 are facultative, with such associations ranging from mutualistic to parasitic from the 103 host's perspective. Among these, Wolbachia is the most common and widespread 104 facultative symbiont of insects and arthropods [24][25][26][27]. 105 106 Wolbachia is a diverse and old genus [possibly older than 200 million years; 28] of 107 intracellular gram-negative Alphaproteobacteria (within the order Rickettsiales) 108 associated with arthropods and filarial nematodes. Wolbachia cells resemble small 109 spheres 0.2-1.5 μ m, occur in all tissue types, but tend to be more prevalent in ovaries 110 and testicles of infected hosts, and are closely associated with the female germline 111 [...

show abstract

Wolbachia strains in cryptic species of the Anastrepha fraterculus complex (Diptera, Tephritidae) along the Neotropical Region

Cited by 12 publications

References 60 publications

Reproductive compatibility among Mexican populations of Anastrepha obliqua: theoretical and management implications

Reproductive compatibility among Mexican populations of Anastrepha obliqua: theoretical and management implications

Wolbachiainfection in Argentinean populations ofAnastrepha fraterculus sp1: preliminary evidence of sex ratio distortion by one of two strains

Wolbachia pipientisassociated to tephritid fruit fly pests: from basic research to applications

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