Wolbachia infection status and genetic structure in natural populations of Polytremis nascens (Lepidoptera: Hesperiidae)

Jiang, Weibin; Zhu, Jianqing; Chen, Minghan; Yang, Qichang; Du, Xuan; Chen, Shiyan; Zhang, Lina; Yu, Yiming; Yu, Weidong

doi:10.1016/j.meegid.2014.07.026

Cited by 21 publications

(20 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The method of dissection was carried out following Kador et al [41]. The DNA was isolated from the dissected body parts and tissues using a QIAamp DNA Mini kit (Qiagen, Hilden, Germany) following the manufacturer's instructions [42].…”

Section: Samples and Dna Extractionmentioning

confidence: 99%

Phylogeny and Density Dynamics of Wolbachia Infection of the Health Pest Paederus fuscipes Curtis (Coleoptera: Staphylinidae)

Zhao

et al. 2020

Insects

Self Cite

View full text Add to dashboard Cite

The maternally inherited obligate intracellular bacteria Wolbachia infects the reproductive tissues of a wide range of arthropods and affects host reproduction. Wolbachia is a credible biocontrol agent for reducing the impact of diseases associated with arthropod vectors. Paederus fuscipes is a small staphylinid beetle that causes dermatitis linearis and conjunctivitis in humans when they come into contact with skin. Wolbachia occur in this beetle, but their relatedness to other Wolbachia, their infection dynamics, and their potential host effects remain unknown. In this study, we report the phylogenetic position and density dynamics of Wolbachia in P. fuscipes. The phylogeny of Wolbachia based on an analysis of MLST genotyping showed that the bacteria from P. fuscipes belong to supergroup B. Quantitative PCR indicated that the infection density in adults was higher than in any other life stage (egg, larva or pupa), and that reproductive tissue in adults had the highest infection densities, with similar densities in the sexes. These findings provide a starting point for understanding the Wolbachia infection dynamics in P. fuscipes, and interactions with other components of the microbiota.

show abstract

Section: Samples and Dna Extractionmentioning

confidence: 99%

Phylogeny and Density Dynamics of Wolbachia Infection of the Health Pest Paederus fuscipes Curtis (Coleoptera: Staphylinidae)

Zhao

et al. 2020

Insects

Self Cite

View full text Add to dashboard Cite

show abstract

“…Given the high levels of intraspecific diversity found in several species in this study, delimitations matching current taxonomy may not be the most successful, but rather an underestimate of the true species number. A full assessment of the taxonomic status of these species will require nuclear and morphological data, as the COI barcode is comparatively short and susceptible to the skewed inheritance patterns resulting from Wolbachia infection 64,65 . Nevertheless, we can make some assessments of the relative merits of these methods as applied to this dataset.…”

Section: Discussionmentioning

confidence: 99%

Towards a global DNA barcode reference library for quarantine identifications of lepidopteran stemborers, with an emphasis on sugarcane pests

Lee

Anderson²,

Tran-Nguyen

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Lepidopteran stemborers are among the most damaging agricultural pests worldwide, able to reduce crop yields by up to 40%. Sugarcane is the world’s most prolific crop, and several stemborer species from the families Noctuidae, Tortricidae, Crambidae and Pyralidae attack sugarcane. Australia is currently free of the most damaging stemborers, but biosecurity efforts are hampered by the difficulty in morphologically distinguishing stemborer species. Here we assess the utility of DNA barcoding in identifying stemborer pest species. We review the current state of the COI barcode sequence library for sugarcane stemborers, assembling a dataset of 1297 sequences from 64 species. Sequences were from specimens collected and identified in this study, downloaded from BOLD or requested from other authors. We performed species delimitation analyses to assess species diversity and the effectiveness of barcoding in this group. Seven species exhibited <0.03 K2P interspecific diversity, indicating that diagnostic barcoding will work well in most of the studied taxa. We identified 24 instances of identification errors in the online database, which has hampered unambiguous stemborer identification using barcodes. Instances of very high within-species diversity indicate that nuclear markers (e.g. 18S, 28S) and additional morphological data (genitalia dissection of all lineages) are needed to confirm species boundaries.

show abstract

“…The DNA was isolated from legs of adult butterflies using a QIAamp DNA Mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. The mitochondrial genes cytochrome oxidase subunit I (COI), cytochrome oxidase subunit II (COII) and three expansion segments (known as variable domains) of nuclear DNA (D3 region of 28S rDNA, V4 and V7 regions of 18S rDNA) were amplified using the primers described in Table 1 [19,20,21]. The PCR conditions were carried out following Jiang et al [22].…”

Section: Methodsmentioning

confidence: 99%

Molecular Phylogeny and Historical Biogeography of the Butterfly Tribe Aeromachini Tutt (Lepidoptera: Hesperiidae) from China

Zhu²,

et al. 2019

Cells

Self Cite

View full text Add to dashboard Cite

: The butterfly tribe Aeromachini Tutt, 1906 is a large group of skippers. In this study, a total of 10 genera and 45 species of putative members of this tribe, which represent most of the generic diversity and nearly all the species diversity of the group in China, were sequenced for two mitochondrial genes and three nuclear genes (2093 bp). The combined dataset was analyzed with maximum likelihood inference using IQtree. We found strong support for monophyly of Aeromachini from China and support for the most recent accepted species in the tribe. Two paraphyletic genera within Aeromachini are presented and discussed. The divergence time estimates with BEAST and ancestral-area reconstructions with RASP provide a detailed description about the historical biogeography of the Aeromachini from China. The tribe very likely originated from the Hengduan Mountains in the late Ecocene and expanded to the Himalaya Mountains and Central China Regions. A dispersal-vicariance analysis suggests that dispersal events have played essential roles in the distribution of extant species, and geological and climatic changes have been important factors driving current distribution patterns.

show abstract

Wolbachia infection status and genetic structure in natural populations of Polytremis nascens (Lepidoptera: Hesperiidae)

Cited by 21 publications

References 77 publications

Phylogeny and Density Dynamics of Wolbachia Infection of the Health Pest Paederus fuscipes Curtis (Coleoptera: Staphylinidae)

Phylogeny and Density Dynamics of Wolbachia Infection of the Health Pest Paederus fuscipes Curtis (Coleoptera: Staphylinidae)

Towards a global DNA barcode reference library for quarantine identifications of lepidopteran stemborers, with an emphasis on sugarcane pests

Molecular Phylogeny and Historical Biogeography of the Butterfly Tribe Aeromachini Tutt (Lepidoptera: Hesperiidae) from China

Contact Info

Product

Resources

About