Wogonoside induces autophagy in MDA-MB-231 cells by regulating MAPK-mTOR pathway

Sun, Yajing; Zou, Meijuan; Hu, Chen; Qin, Yansu; Song, Xiuming; Lu, Na; Guo, Qinglong

doi:10.1016/j.fct.2012.09.012

Cited by 42 publications

(28 citation statements)

References 37 publications

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“…Huang et al (17) demonstrated that wogonoside inhibits angiogenesis via suppressing Wnt/β-catenin pathway in breast cancer. Sun et al (21) suggested that wogonoside induces autophagy through regulating the MAPK-mTOR signaling pathway in MDA-MB-231 cells.…”

Section: Discussionmentioning

confidence: 99%

“…In the present, the results demonstrated that wogonoside significantly suppressed p-mTOR protein expression in LOVO cells. Sun et al (21) suggested that wogonoside induces autophagy through regulating the mitogen-activated protein kinase-mTOR signaling pathway in MDA-MB-231 cells. P70S6K, the kinase of 40S ribosomal small subunit protein S6 and one of the downstream targets in the PI3K/Akt/mTOR signaling pathway, directly phosphorylates 40S ribosomal protein S6 to promote the translation of mRNA essential in the Gl-S stage of the cell cycle, as well as the expression of proteins associated with cell growth and differentiation, thus serving an important role in the regulation of cell growth and proliferation (29,30).…”

Section: Discussionmentioning

confidence: 99%

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Wogonoside inhibits cell growth and induces mitochondrial-mediated autophagy-related apoptosis in human colon cancer cells through the PI3K/AKT/mTOR/p70S6K signaling pathway

et al. 2018

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Abstract. Wogonoside, the main effective constituent of traditional Chinese medicine Scutellaria, belongs to the glucuronide family, with various functions, including detoxification, anti-inflammation and nourishing gallbladder, lowering blood pressure, diuresis and anti-allergic reactions. However, the effects of wogonoside on human colon cancer cells remain unclear. The present study aimed to investigate the anticancer effect of wogonoside on human colon cancer cells in vitro and its anticancer mechanisms. The results demonstrated that wogonoside significantly inhibited cell growth, induced apoptosis and mitochondrial-mediated autophagy of colon cancer cells. Furthermore, the results revealed that wogonoside significantly increased caspase-3 and caspase-9 expression levels, induced apoptosis regulator Bax/Bcl-2 and microtubule-associated protein 1A/1B-light chain 3 protein expression, suppressed the phosphatidylinositol 3 kinase (PI3K)/RAC-α serine/threonine-protein kinase (Akt)/mechanistic target of rapamycin (mTOR)/p70 S6 kinase (p70S6K) signaling pathway and induced p62 protein expression in colon cancer cells. In conclusion, these results demonstrated that wogonoside inhibits cell growth and induces mitochondrial mediated autophagy-related apoptosis in human colon cancer cells through modulation of the PI3K/Akt/mTOR/p70S6K signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Wogonoside inhibits cell growth and induces mitochondrial-mediated autophagy-related apoptosis in human colon cancer cells through the PI3K/AKT/mTOR/p70S6K signaling pathway

et al. 2018

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“…The role of JNK in tumor development remained controversial, although it was implicated in oncogenic transformation (Zhang et al, 2012). Published data implied that JNK pathway was involved in the human breast cancer cell (Sun et al, 2013), hepatoma cancer cell , and so on. And also, MAPK pathway could activate its downstream Fas/ FasL signaling pathway and induce the apoptosis of cancer cells (Antonsson et al, 2000;Chen et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Glaucocalyxin A Activates FasL and Induces Apoptosis Through Activation of the JNK Pathway in Human Breast Cancer Cells

Jiang²,

Z³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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This study was conducted to analyze the molecular mechanisms responsible for anti-proliferation effects of glaucocalyxin A in cultured MCF-7 and Hs578T breast cancer cells. The concentration that reduced cell viability to 50% (IC50) after 72 h treatment was derived and potential molecular mechanisms of anti-proliferation using the IC50 were investigated as changes in cell cycle arrest and apoptosis. Gene and protein expression changes related to apoptosis were investigated by semi-quantitative RT-PCR and western blotting, respectively. Involvement of phosphorylated mitogen-activated protein kinases and JNK signaling in regulation of these molecules was characterized by western blotting. Cell viability decreased in a concentration-dependent manner and the IC50 was determined as 1 μΜ in MCF-7 and 4 μΜ in Hs578T cell. Subsequently, we demonstrated that the GLA-induced MCF-7 and Hst578T cell death was due to cell cycle arrest at the G2/M transition and was associated with activation of the c-jun N-terminal kinase (JNK) pathway. We conclude that GLA has the potential to inhibit the proliferation of human breast cancer cells through the JNK pathway and suggest its application forthe effective therapy for patients with breast cancer.

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“…Increasing evidence has demonstrated that wogonoside, a major extract of Scutellaria baicalensis, induces autophagy in MDA-MB-231 cells by regulating the MAPK-mTOR pathway (19). It also participates in cell cycle arrest and differentiation by regulating the expression and subcellular localization of PLSCR1 in AMl cells (20).…”

Section: Discussionmentioning

confidence: 99%

“…The anticancer effect of Scutellaria baicalensis extract has recently become a topic of interest. Moreover, a number of in vitro studies suggest that the extract of Scutellaria baicalensis inhibits the growth of various cancer cell lines through specific biological signaling pathways, including apoptosis, angiogenesis and inhibition of the androgen receptor (15,16,(18)(19)(20)(21)(22)(23).…”

Section: Discussionmentioning

confidence: 99%

Wogonoside induces apoptosis in Bel-7402, a hepatocellular carcinoma cell line, by regulating Bax/Bcl-2

Chen

et al. 2015

Oncology Letters

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Abstract. The anticancer effect of Scutellaria baicalensis extract has recently become a topic of interest. In this study, the anticancer effects and underlying mechanisms of wogonoside, the main constituent of Scutellaria baicalensis, were investigated in a human hepatocellular carcinoma (HCC) cell line in vitro. The effects of wogonoside on the proliferation, cell cycle progression and apoptosis of hepatocellular carcinoma cells were examined. Western blotting was employed to analyze the proteins associated with the biological effects of wogonoside. Wogonoside exerted anti-proliferation properties in vitro. HCC cell growth was attenuated by wogonoside (8 µM) treatment. Cell cycle progression analysis and DNA ladder assay revealed that apoptosis was enhanced in wogonoside-treated cells and that cell cycle arrest occurred in the G2/M phase. It was also demonstrated that increased apoptosis was accompanied by increased levels of Bax protein and decreased levels of Bcl-2 protein. The results of this study suggest that wogonoside may represent a potential therapeutic agent against HCC.

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Wogonoside induces autophagy in MDA-MB-231 cells by regulating MAPK-mTOR pathway

Cited by 42 publications

References 37 publications

Wogonoside inhibits cell growth and induces mitochondrial-mediated autophagy-related apoptosis in human colon cancer cells through the PI3K/AKT/mTOR/p70S6K signaling pathway

Wogonoside inhibits cell growth and induces mitochondrial-mediated autophagy-related apoptosis in human colon cancer cells through the PI3K/AKT/mTOR/p70S6K signaling pathway

Glaucocalyxin A Activates FasL and Induces Apoptosis Through Activation of the JNK Pathway in Human Breast Cancer Cells

Wogonoside induces apoptosis in Bel-7402, a hepatocellular carcinoma cell line, by regulating Bax/Bcl-2

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