Wogonin enhances intracellular adiponectin levels and suppresses adiponectin secretion in 3T3-L1 adipocytes

Tan, Yang; Liu, Hua; Zhao, Bo; Zhang, Yemin; Zhang, Deling; Li, Mingxin; Zhang, Zhijiang; Bi, Yang; Wang, Changhua

doi:10.1507/endocrj.ej16-0158

Cited by 7 publications

(3 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The human breast cancer cell lines MCF-7 and MDA-MB-231 and HEK 293 T cells were obtained from American Type Culture Collection (ATCC, Shanghai) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% exosome-free foetal bovine serum (FBS, Shin Chin Industrial, SCI) and 1% penicillin–streptomycin (HyClone, Logan, UT, USA) in a humidified 37 °C incubator with 5% CO 2 . 3T3-L1 preadipocytes were obtained from ATCC (Shanghai) and cultured in DMEM supplemented with 10% foetal calf serum (FCS, Gibco) and 1% penicillin–streptomycin (HyClone, Logan, UT, USA) in a humidified 37 °C incubator with 5% CO 2 ; these cells were differentiated as previously reported [15]. Differentiation was confirmed by Oil Red O staining.…”

Section: Methodsmentioning

confidence: 99%

RETRACTED ARTICLE: Exosomes from the tumour-adipocyte interplay stimulate beige/brown differentiation and reprogram metabolism in stromal adipocytes to promote tumour progression

et al. 2019

J Exp Clin Cancer Res

Self Cite

126

164

View full text Add to dashboard Cite

Background Emerging evidence supports the pivotal roles of adipocytes in breast cancer progression. Tumour induced beige/brown adipose tissue differentiation contributes to the hypermetabolic state of the breast cancer. However, the mediators and mechanisms remain unclear. Methods Survival probabilities were estimated using the Kaplan–Meier method based on immunohistochemistry results. Biochemical studies were performed to characterize the novel interrelation between breast cancer cells and adipocytes. Results We show that tumour-surrounding adipocytes exhibit an altered phenotype in terms of upregulated beige/brown characteristics and increased catabolism associated with an activated state characterized by the release of metabolites, including free fatty acids, pyruvate, lactate and ketone bodies. Likewise, tumour cells cocultivated with mature adipocytes exhibit metabolic adaptation and an aggressive phenotype in vitro and in vivo. Mechanistically, we show that tumour cells induce beige/brown differentiation and remodel metabolism in resident adipocytes by exosomes from the co-culture system that carry high levels of miRNA-144 and miRNA-126. miRNA-144 promotes beige/brown adipocyte characteristics by downregulating the MAP3K8/ERK1/2/PPARγ axis, and exosomal miRNA-126 remodels metabolism by disrupting IRS/Glut-4 signalling, activating the AMPK/autophagy pathway and stabilizing HIF1α expression in imminent adipocytes. In vivo inhibition of miRNA-144 or miRNA-126 decreases adipocyte–induced tumour growth. Conclusions These results demonstrate that by inducing beige/brown differentiation and enhancing catabolism in recipient adipocytes, exosomal miRNA-144 and miRNA-126 from the tumour-adipocyte interaction reprogram systemic energy metabolism to facilitate tumour progression. Electronic supplementary material The online version of this article (10.1186/s13046-019-1210-3) contains supplementary material, which is available to authorized users.

show abstract

Section: Methodsmentioning

confidence: 99%

RETRACTED ARTICLE: Exosomes from the tumour-adipocyte interplay stimulate beige/brown differentiation and reprogram metabolism in stromal adipocytes to promote tumour progression

et al. 2019

J Exp Clin Cancer Res

Self Cite

126

164

View full text Add to dashboard Cite

show abstract

“…Chrysin treatment has been used to improve diabetes in rats, which could attenuate diabetes-induced impairment [64]. Wogonin could enhance the intracellular level of adiponectin, a therapeutic target for insulin resistance, diabetes, and diabetes-related complications [65]. Although there were few reports about bioactivity of 5,8,2′trihydroxy-7-methoxyflavone, the activities of flavonoids are structure dependent with the hydroxylated phenolic structure [66], which should be tested in the future.…”

Section: Compound-target-pathway (Ctp) Network and Main Active Constimentioning

confidence: 99%

A systematic analysis of natural α-glucosidase inhibitors from flavonoids of Radix scutellariae using ultrafiltration UPLC-TripleTOF-MS/MS and network pharmacology

Wang

Tan

Wang

et al. 2020

BMC Complement Med Ther

View full text Add to dashboard Cite

Background: Flavonoids from plant medicines are supposed to be viable alternatives for the treatment of type 2 diabetes (T2D) as less toxicity and side effects. Radix scutellariae (RS) is a widely used traditional medicine in Asia. It has shown great potential in the research of T2D. However, the pharmacological actions remain obscured due to the complex chemical nature of plant medicines.Methods: In the present study, a systematic method combining ultrafiltration UPLC-TripleTOF-MS/MS and network pharmacology was developed to screen α-glucosidase inhibitors from flavonoids of RS, and explore the underlying mechanism for the treatment of T2D.Results: The n-butanol part of ethanol extract from RS showed a strong α-glucosidase inhibition activity (90.55%, IC 50 0.551 mg/mL) against positive control acarbose (90.59%, IC 50 1.079 mg/mL). A total of 32 kinds of flavonoids were identified from the extract, and their ESI-MS/MS behaviors were elucidated. Thirteen compounds were screened as α-glucosidase inhibitors, including viscidulin III, 2′,3,5,6′,7-pentahydroxyflavanone, and so on. A compound-target-pathway (CTP) network was constructed by integrating these α-glucosidase inhibitors, target proteins, and related pathways. This network exhibited an uneven distribution and approximate scale-free property. Chrysin (k = 87), 5,8,2′-trihydroxy-7-methoxyflavone (k = 21) and wogonin (k = 20) were selected as the main active constituents with much higher degree values. A protein-protein interaction (PPI) weighted network was built for target proteins of these α-glucosidase inhibitors and drug targets of T2D. PPARG (C d = 0.165, C b = 0.232, C c = 0.401), ACACB (C d = 0.155, C b = 0.184, C c = 0.318), NFKB1 (C d = 0.233, C b = 0.161, C c = 0.431), and PGH2 (C d = 0.194, C b = 0.157, C c = 0.427) exhibited as key targets with the highest scores of centrality indices. Furthermore, a core subnetwork was extracted from the CTP and PPI weighted network. Type II diabetes mellitus (hsa04930) and PPAR signaling pathway (hsa03320) were confirmed as the critical pathways.(Continued on next page) Conclusions: These results improved current understanding of natural flavonoids on the treatment of T2D. The combination of ultrafiltration UPLC-TripleTOF-MS/MS and network pharmacology provides a novel strategy for the research of plant medicines and complex diseases.

show abstract

“…Sirtuin 1 (SIRT1), the downstream effector of adenosine monophosphate activated protein kinase (AMPK), has been suggested to play significant roles in the regulation of adiponectin release [ 14 ]. Wogonin supplementation remarkably increased the AMPK phosphorylation and SIRT1 expression, whereas inhibition of AMPK or SIRT1 reduced the effects of wogonin on the production or release of adiponectin [ 14 ]. It was previously reported that SIRT1-modulated HMGB1 deacetylation inhibited acute kidney injury incurred by sepsis [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Protective effects of wogonin on lipopolysaccharide-induced inflammation and apoptosis of lung epithelial cells and its possible mechanisms

et al. 2021

View full text Add to dashboard Cite

Background Wogonin (5, 7-dihydroxy-8-methoxyflavone) is a natural di-hydroxyl flavonoid extracted from the root of Scutellaria baicalensis Georgi. This paper was intended to investigate the mechanism of action of wogonin in alleviating the inflammation and apoptosis in acute lung injury (ALI). Materials and methods Lipopolysaccharide (LPS) was used to establish the in vitro model of ALI. After wogonin treatment, the cell viability and apoptosis of LPS-induced A549 cells were, respectively, measured by CCK-8, TUNEL assays and acridine orange/ethidium bromide dual staining, while the contents of inflammatory cytokines and oxidative stress markers were estimated by RT-qPCR, ELISA assay, western blot analysis and commercial kits. Western blot was also conducted to assess the expression of proteins involved. Subsequently, the effect of wogonin on the sirtuin 1 (SIRT1)-mediated high-mobility group box 1 protein (HMGB1) deacetylation was investigated. SIRT1 inhibitor EX527 was used to evaluate the regulatory effects of wogonin on SIRT1-mediated HMGB1 deacetylation in A549 cells under LPS stimulation. Results LPS induced inflammation, oxidative stress and apoptosis of A549 cells, which was abolished by wogonin. It was also found that wogonin promoted the HMGB1 deacetylation, accompanied by upregulated SIRT1 expression. However, SIRT1 inhibitor EX527 partially reversed the protective effects of wogonin on the inflammation and apoptosis of LPS-induced A549 cells. Conclusion Wogonin alleviated the inflammation and apoptosis in LPS-induced A549 cells by SIRT1-mediated HMGB1 deacetylation, which might represent the identification of a novel mechanism by which wogonin exerts protective effects on ALI and provide ideas for the application of wogonin to ALI treatment.

show abstract

Wogonin enhances intracellular adiponectin levels and suppresses adiponectin secretion in 3T3-L1 adipocytes

Cited by 7 publications

References 49 publications

RETRACTED ARTICLE: Exosomes from the tumour-adipocyte interplay stimulate beige/brown differentiation and reprogram metabolism in stromal adipocytes to promote tumour progression

RETRACTED ARTICLE: Exosomes from the tumour-adipocyte interplay stimulate beige/brown differentiation and reprogram metabolism in stromal adipocytes to promote tumour progression

A systematic analysis of natural α-glucosidase inhibitors from flavonoids of Radix scutellariae using ultrafiltration UPLC-TripleTOF-MS/MS and network pharmacology

Protective effects of wogonin on lipopolysaccharide-induced inflammation and apoptosis of lung epithelial cells and its possible mechanisms

Contact Info

Product

Resources

About