Wogonin attenuates liver fibrosis via regulating hepatic stellate cell activation and apoptosis

Du, Xiao‐Sa; Li, Hai-Di; Yang, Xinye; Li, Juanjuan; Xu, Jiejie; Chen, Yu; Xu, Qingqing; Yang, Lei; He, Chang-Sheng; Huang, Cheng; Meng, Xiao‐Ming

doi:10.1016/j.intimp.2019.05.056

Cited by 46 publications

(29 citation statements)

References 39 publications

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“…Wogonin attenuated tubular damage, suppressed kidney inflammation and inhibited NF-κB activation in the injured kidney, thereby exhibiting the renoprotective effect [46] and it is a promising nephroprotective agent [47] . Pro-apoptosis effect of wogonin in vivo was verified in situ [48] . Wogonin exhibits a cytotoxic effect on lung cancer cells [49] and exerts potent anticancer effects on colorectal cancer cells [50] .…”

Section: Discussionmentioning

confidence: 84%

Analysis of the mechanism of Shufeng Jiedu capsule prevention and treatment for COVID-19 by network pharmacology tools

Xiong

Dong

Lou

et al. 2020

European Journal of Integrative Medicine

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Introduction The novel coronavirus pneumonia that broke out in 2019 has become a global epidemic. According to the diagnosis and treatment plan issued in China and the existing clinical data, Shufeng Jiedu (SFJD) Capsule can be effectively used in the treatment of COVID-19 patients. This study aimed to explore its mechanism of action by network pharmacology and molecular docking technology. Methods The Chinese Medicine System Pharmacology Analysis Platform (TCMSP), a Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine (BATMAN-TCM), the Encyclopedia of Traditional Chinese Medicine (ETCM) and related literature records were used to search the composition and main active compounds of SFJD, and to screen out the targets of drug components. Disease-associated genes were obtained by the Human Gene Database (GeneCards), the Human Online Mendelian Inheritance Platform (OMIM) and the DisGeNET database, and the co-targeted genes/proteins as targets of both SFJD and COVID-19 were selected by the Comparative Toxicogenomics Database (CTD). Co-targeted genes/proteins were analyzed by STRING, the Database for Annotation, Visualization and Integrated Discovery (DAVID) and Reactome for proteins to protein interaction (PPI), pathway and GO (gene ontology) enrichment, and predicted by AutoDock for their high-precision docking simulation. In addition, the therapeutic effect for SFJD treatment on COVID-19 was validated by the Chinese medicine anti-novel coronavirus pneumonia drug effect prediction and analysis platform (TCMCOVID). Results Screening resulted in 163 compounds and 463 targeted genes. The PPI core network contains 76 co-targeted proteins. The Reactome pathways were enriched in signaling by interleukins, immune system, etc. Finally, 6 key proteins of TNF, IL-10, IL-2, IL-6, STAT1 and CCL2 were selected and successfully docked with 4 active ingredients of quercetin, luteolin, wogonin and kaempferol. Conclusion SFJD may play a role in the prevention and treatment of COVID-19 through multiple active compounds acting on multiple targets and then multiple pathways.

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Section: Discussionmentioning

confidence: 84%

Analysis of the mechanism of Shufeng Jiedu capsule prevention and treatment for COVID-19 by network pharmacology tools

Xiong

Dong

Lou

et al. 2020

European Journal of Integrative Medicine

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“…The expressions of MMPs have been found to increase in and associated with a variety of inflammatory diseases 39,40 . Previous studies have shown that MMP-13 was highly expressed in OA patients, suggesting that increased MMP-13 is related to cartilage degradation 41,42 . Studies showed that discoidin domain receptor 2 (DDR2) and MMP-13 may play an important role in the development of tumor angiogenesis.…”

Section: Discussionmentioning

confidence: 98%

Identification of Differential Expression Cytokines in Hemolysis, Elevated Liver Enzymes, and Low Platelet Syndrome by Proteome Microarray Analysis and Further Verification

et al. 2021

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To screen the differential expression cytokines (DECs) in hemolysis, elevated liver enzymes, and low platelet (HELLP) syndrome, establish its differential cytokines spectra, and provide the clues for its diagnosis and pathogenic mechanism researches. Sera from four HELLP syndrome patients and four healthy controls were detected by proteome microarray. Then the analysis of Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and protein–protein interaction (PPI) network were performed and possible hub proteins were selected out, further verified by Enzyme Linked Immunosorbent Assay (ELISA) in sera from 21 HELLP syndrome patients and 21 healthy controls. Thirty DECs were defined according to P-value and fold change between HELLP group and control group. GO enrichment analysis showed that DECs were mainly involved in the regulation of inflammatory response and have relationship to growth factor binding, transmembrane receptor protein kinase, and cytokine receptor activity. Seven possible hub proteins were defined by PPI analysis, including IGFBP-3/Follistatin-like 1/FLRG/Fetuin A and MMP-13/Thrombospondin-5/Aggrecan. ELISA showed higher serum levels of Fetuin A/IGFBP-3/FLGR/MMP-13/Thrombospondin-5 in HELLP group than those in controls, while the levels of Follistatin-like 1 and Aggrecan were lower in HELLP patients (all P < 0.05 or <0.01).The serological DECs spectra of HELLP syndrome was established and seven possible hub proteins that may be more closely related to the disease have been verified, providing new clues for its pathogenesis, diagnosis, and clinical treatment.

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“…Together with phytol, luteolin, and hispidulin, wogonin is one of the active constituents in the chloroform fraction of SB, which has demonstrated dose-dependent cytotoxicity on six cancer cell lines [ 74 ]. Wogonin presents anti-viral activities against influenza infection via modulation of AMP-activated protein kinase (AMPK) pathways, and anti-fibrotic effects in liver fibrosis via regulating the activation and apoptosis of hepatic stellate cells [ 75 ]. Thus, wogonin may present a therapeutic option to attenuate the cytokine storm in acute symptomatic SARS-CoV-2 infections due to the immunomodulatory response [ 76 ] and its anti-fibrotic effects in the convalescence period [ 75 ].…”

Section: Discussionmentioning

confidence: 99%

“…Wogonin presents anti-viral activities against influenza infection via modulation of AMP-activated protein kinase (AMPK) pathways, and anti-fibrotic effects in liver fibrosis via regulating the activation and apoptosis of hepatic stellate cells [ 75 ]. Thus, wogonin may present a therapeutic option to attenuate the cytokine storm in acute symptomatic SARS-CoV-2 infections due to the immunomodulatory response [ 76 ] and its anti-fibrotic effects in the convalescence period [ 75 ]. Yet another flavonoid compound for quality control in SB [ 8 ], naringenin, can inhibit two-pore channels (TPCs) [ 77 , 78 ], which regulate the trafficking of the virus to late-endosomes/lysosomes following entry into cells [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

Scutellaria barbata D. Don Inhibits the Main Proteases (Mpro and TMPRSS2) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection

Huang

Chen

Chang

et al. 2021

Viruses

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In late 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic emerged to severely impact the global population, creating an unprecedented need for effective treatments. This study aims to investigate the potential of Scutellaria barbata D. Don (SB) as a treatment for SARS-CoV-2 infection through the inhibition of the proteases playing important functions in the infection by SARS-CoV-2. FRET assay was applied to investigate the inhibitory effects of SB on the two proteases involved in SARS-CoV-2 infection, Mpro and TMPRSS2. Additionally, to measure the potential effectiveness of SB treatment on infection inhibition, cellular models based on the Calu3 and VeroE6 cells and their TMPRSS2- expressing derivatives were assessed by viral pseudoparticles (Vpp) infection assays. The experimental approaches were conjugated with LC/MS analyses of the aqueous extracts of SB to identify the major constituent compounds, followed by a literature review to determine the potential active components of the inhibitory effects on protease activities. Our results showed that SB extracts inhibited the enzyme activities of Mpro and TMPRSS2. Furthermore, SB extracts effectively inhibited SARS-CoV-2 Vpp infection through a TMPRSS2-dependent mechanism. The aqueous extract analysis identified six major constituent compounds present in SB. Some of them have been known associated with inhibitory activities of TMPRSS2 or Mpro. Thus, SB may effectively prevent SARS-CoV-2 infection and replication through inhibiting Mpro and TMPRSS2 protease activities.

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Wogonin attenuates liver fibrosis via regulating hepatic stellate cell activation and apoptosis

Cited by 46 publications

References 39 publications

Analysis of the mechanism of Shufeng Jiedu capsule prevention and treatment for COVID-19 by network pharmacology tools

Analysis of the mechanism of Shufeng Jiedu capsule prevention and treatment for COVID-19 by network pharmacology tools

Identification of Differential Expression Cytokines in Hemolysis, Elevated Liver Enzymes, and Low Platelet Syndrome by Proteome Microarray Analysis and Further Verification

Scutellaria barbata D. Don Inhibits the Main Proteases (Mpro and TMPRSS2) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection

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