Wogonin and fisetin induce apoptosis in human promyeloleukemic cells, accompanied by a decrease of reactive oxygen species, and activation of caspase 3 and Ca2+-dependent endonuclease

Lee, William R.; Shen, Shing Chuan; Lin, Hui Yi; Hou, Wen Chi; Yang, Ling; Chen, Yen Chou

doi:10.1016/s0006-2952(01)00876-0

Cited by 210 publications

(158 citation statements)

References 44 publications

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“…In contrast to 14 C-5S-HETE, the biosynthetic capabilities for 14 C-15S-/12S-HETE were not affected after either alcohol or drug pretreatment.…”

Section: Resultsmentioning

confidence: 68%

“…Various irritants including alcohol produce gastric mucosal damage by both apoptosis and necrosis (25). A previous study demonstrated that wogonin induced apoptosis through activation of endonuclease, caspase-3, or proapoptotic protein, Bax, in human promyeloleukemic cells (14). These speculations led us to examine the effect of wogonin on ethanol-induced gastric mucosal apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Although several studies demonstrated that wogonin exerted either antiinflammatory (7-9) or proapoptotic actions (14) in vitro or in vivo, none has reported wogonin's effect in the gastric environment and disease. Our results reveal that the protective effect of wogonin against gastric mucosal damages could be accomplished by either dual actions on AA metabolism via both COX and LOX pathways or preventive apoptosis induction.…”

Section: Discussionmentioning

confidence: 99%

“…Protein concentrations of each fraction were determined using Bio-Rad reagent, with bovine serum albumin as standard. One hundred micrograms of microsomal protein was mixed with COX buffer and preincubated at room temperature for 5 min, while 14 fraction to the 14 C-AA and then incubated at 37 • C for 30 min in a shaking water bath. The incubation was terminated by acidification with 50 µl of ice-cold 0.15 N HCl.…”

Section: Methodsmentioning

confidence: 99%

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Preventive Effect of the Flavonoid, Wogonin, Against Ethanol-Induced Gastric Mucosal Damage in Rats

Park

Hahm

et al. 2004

Dig Dis Sci

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Whether wogonin (5,7-dihydroxy-8-methoxyflavone), a flavonoid originated from the root of Scutellaria baicalensis Georgi, which has been shown to have antiinflammatory and antitumor activities in various cell types, possesses a gastric cytoprotective effect was investigated in an ethanol-induced gastric damage model in rats. Ethanol administration alone induced evident gastric damage including gastric hemorrhages and edema, while this gastric damage was significantly attenuated by wogonin pretreatment (30 mg/kg B.W.) 1 hr before ethanol administration. As major protective mechanisms of wogonin on ethanol-induced gastric damage, we found that wogonin showed either antiinflammatory effects through dual actions on arachidonic acid metabolism, i.e., induction of prostaglandin D 2 and suppression of 5S-hydroxyeicosatetraenoic acid (5S-HETE), or preventive induction of profuse apoptosis in the stomach. Conclusively, the flavonoid wogonin could be used as a preventive agent of alcohol-induced gastropathy, whose actions were proven to be strong antiinflammation and apoptosis induction.

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“…In contrast to 14 C-5S-HETE, the biosynthetic capabilities for 14 C-15S-/12S-HETE were not affected after either alcohol or drug pretreatment.…”

Section: Resultsmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Preventive Effect of the Flavonoid, Wogonin, Against Ethanol-Induced Gastric Mucosal Damage in Rats

Park

Hahm

et al. 2004

Dig Dis Sci

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“…Recent research have reported anti-proliferative (Chen et al, 2002;Haddad et al, 2006) and anti-metastasic effects (Chou et al, 2013) of fisetin, a polyphenolic flavonoid present in many fruits such as strawberries and cucumbers (Kim et al, 2012). Mechanisms through which fisetin exerts its action is yet to be elucidated completely, although it has been demonstrated to increase expressions of p53, down-regulate cyclin D1 and cyclin E levels (Chen et al, 2002;Lee et al, 2002;Lu et al, 2005b;Haddad et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Fisetin, a dietary flavonoid induces apoptosis via modulating the MAPK and PI3K/Akt signalling pathways in human osteosarcoma (U-2 OS) cells

Huang

et al. 2015

Bangladesh J Pharmacol

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Human osteosarcoma is the most prevalent primary malignant bone tumor with high frequency of invasion and metastasis. Strong resistance coupled with toxicity of the currently available chemotherapeutic drugs poses challenge in treatment. The study aimed to investigate if fisetin, a dietary flavonoid induced apoptosis in human osteosarcoma (U-2 OS) cells. Fisetin at 20-100 µM effectively reduced the viability of OS cells, and induced apoptosis by significantly inducing the expression of caspases (Caspases-3,-8 and -9) and proapoptotic proteins (Bax and Bad) with subsequent down-regulation of Bcl-xL and Bcl-2. While fisetin inhibited PI3K/Akt pathway and ERK1/2, it caused enhanced expressions of p-JNK, p-c-Jun and p-p38. Fisetin-induced ROS generation and decrease in mitochondrial membrane potential would have also contributed to rise in apoptotic cell counts. The observations suggest that fisetin was able to effectively induce apoptosis of U-2 OS cells through ROS generation and modulation of MAPK and PI3K/Akt signalling cascades. Article Info

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Pharmacological effects and pharmacokinetics properties ofRadix Scutellariaeand its bioactive flavones

Lin

Zuo

2011

Biopharm & Drug Disp

219

129

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Radix Scutellariae is the dried root of the medicinal plant Scutellariae baicalensis Georgi. It exhibits a variety of therapeutic effects and has a long history of application in traditional formulations as well as in modern herbal medications. It has been confirmed that flavonoids are the most abundant constituents and induce these therapeutic effects. Six flavones are proven to be the major bioactive flavones in Radix Scutellariae existing in the forms of aglycones (baicalein, wogonin, oroxylin A) and glycosides (baicalin, wogonoside, oroxylin A-7-glucuronide). All six flavones are pharmacologically active and show great potential in the treatment of inflammation, cancers and virus-related diseases. The current review covers the preparation of the herb Radix Scutellariae, quantification of its major bioactive ingredients, and pharmacological effects of the proposed six bioactive flavones. In addition, this review summarizes the pharmacokinetic profiles of the bioactive flavones reported so far that could be used for further improvement of their pharmacokinetic study. Moreover, due to abundant co-occurring bioactive components in Radix Scutellariae, our review further documents the pharmacokinetic interactions among them.

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Wogonin and fisetin induce apoptosis in human promyeloleukemic cells, accompanied by a decrease of reactive oxygen species, and activation of caspase 3 and Ca2+-dependent endonuclease

Cited by 210 publications

References 44 publications

Preventive Effect of the Flavonoid, Wogonin, Against Ethanol-Induced Gastric Mucosal Damage in Rats

Preventive Effect of the Flavonoid, Wogonin, Against Ethanol-Induced Gastric Mucosal Damage in Rats

Fisetin, a dietary flavonoid induces apoptosis via modulating the MAPK and PI3K/Akt signalling pathways in human osteosarcoma (U-2 OS) cells

Pharmacological effects and pharmacokinetics properties ofRadix Scutellariaeand its bioactive flavones

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