WNT8B as an Independent Prognostic Marker for Nasopharyngeal Carcinoma

Ngernsombat, Chawalit; Prattapong, Pongphol; Larbcharoensub, Noppadol; Khotthong, Krittika; Janvilisri, Tavan

doi:10.3390/curroncol28040230

Cited by 3 publications

(6 citation statements)

References 60 publications

(65 reference statements)

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“…Our transcriptomic analyses resulted in the considerable identification of the Wnt signaling pathway using pathway enrichment analysis. Several Wnt signaling pathway members have previously been identified as being up-regulated in several types of cancers, including NPC ( 19 , 33 – 35 ). Remarkably, the relapse cases of NPC exhibited the different expression of Wnt signaling compared to the point of diagnosis, including various WNT isoforms, FZD10 , CTNNB1 , and AXIN2 expression.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, up-regulation of WNT5A has recently been reported in primary NPC tissue samples and cell lines associated with EBV infection ( 36 ), which promotes aggressiveness and stem characteristics in NPC ( 37 ). Our previous tissue microarray analysis illustrated that WNT8B is associated with NPC patients’ survival, which could be used as a prognostic biomarker for NPC patients ( 19 ). As the NPC development comprises multi-step carcinogenesis, the differential expression of Wnt components in our studies may affect the mechanisms of NPC pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Tissue microarrays containing 86 human NPC and 6 normal nasopharyngeal epithelial tissues were constructed using paraffin blocks from Ramathibodi Hospital as described previously ( 19 ). Briefly, 5 μm TMA sections were blocked with bovine serum albumin and incubated with rabbit anti-FZD10 antibody (1:200 dilution; catalog number ab83044, Abcam, USA) at 4°C overnight in a humidified chamber.…”

Section: Methodsmentioning

confidence: 99%

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Transcriptomic profiling revealed FZD10 as a novel biomarker for nasopharyngeal carcinoma recurrence

Tulalamba

Ngernsombat²,

Larbcharoensub³

et al. 2023

Front. Oncol.

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BackgroundNasopharyngeal carcinoma (NPC) is a type of cancers that develops in the nasopharynx, the very upper part of the throat behind the nose. NPC is typically diagnosed in later stages of the disease and has a high rate of recurrence due to the location of the tumor growth site. In this study, we compared the gene expression profiles of NPC tissues from patients with and without recurrence to identify potential molecular biomarkers of NPC recurrence.MethodsMicroarrays were used to analyze the expression of genes in 15 NPC tissues taken at the time of diagnosis and at the site of recurrence following therapeutic treatment. Pathway enrichment analysis was used to examine the biological interactions between the major differentially expressed genes. The target identified was then validated using immunohistochemistry on 86 NPC tissue samples.ResultsOur data showed that the Wnt signaling pathway was enhanced in NPC tissues with recurrence. FZD10, a component of the Wnt signaling pathway, was significantly expressed in NPC tissues, and was significantly associated with NPC recurrence.ConclusionOur study provides new insights into the pathogenesis of NPC and identifies FZD10 as a potential molecular biomarker for NPC recurrence. FZD10 may be a promising candidate for NPC recurrence and a potential therapeutic target.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Transcriptomic profiling revealed FZD10 as a novel biomarker for nasopharyngeal carcinoma recurrence

Tulalamba

Ngernsombat²,

Larbcharoensub³

et al. 2023

Front. Oncol.

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“…The aberrant upregulation of Wnt/β-catenin signalling was observed in NPC through the examination of the expression of canonical WNT ligands, such as WNT8B. In the study, a substantial 75.6% of NPC cases were exhibited high expression of WNT8B [ 293 ]. Subsequent observations of NPC patients with overexpressed WNT8B revealed a correlation with shorter survival durations.…”

Section: Molecular Aberrations Exploitation In Npc For Precision Medi...mentioning

confidence: 99%

Precision medicine in nasopharyngeal carcinoma: comprehensive review of past, present, and future prospect

Siak,

Heng,

Teoh

et al. 2023

J Transl Med

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Nasopharyngeal carcinoma (NPC) is an aggressive malignancy with high propensity for lymphatic spread and distant metastasis. It is prominent as an endemic malignancy in Southern China and Southeast Asia regions. Studies on NPC pathogenesis mechanism in the past decades such as through Epstein Barr Virus (EBV) infection and oncogenic molecular aberrations have explored several potential targets for therapy and diagnosis. The EBV infection introduces oncoviral proteins that consequently hyperactivate many promitotic pathways and block cell-death inducers. EBV infection is so prevalent in NPC patients such that EBV serological tests were used to diagnose and screen NPC patients. On the other hand, as the downstream effectors of oncogenic mechanisms, the promitotic pathways can potentially be exploited therapeutically. With the apparent heterogeneity and distinct molecular aberrations of NPC tumor, the focus has turned into a more personalized treatment in NPC. Herein in this comprehensive review, we depict the current status of screening, diagnosis, treatment, and prevention in NPC. Subsequently, based on the limitations on those aspects, we look at their potential improvements in moving towards the path of precision medicine. The importance of recent advances on the key molecular aberration involved in pathogenesis of NPC for precision medicine progression has also been reported in the present review. Besides, the challenge and future outlook of NPC management will also be highlighted.

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“…miRNAs are attractive therapeutic targets, either as oncogenes or as repressors. Previous studies have shown that miR-4757-3p is a target miRNA of Wnt5a or Wnt8b in some tumors [ 7 , 8 ]. The marker molecules of the Wnt signaling pathway, Wnt5a, and Wnt8b are required for progression of cancer [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

miR-4757-3p Inhibited the Migration and Invasion of Lung Cancer Cell via Targeting Wnt Signaling Pathway

Zhao

Chen

et al. 2023

Journal of Oncology

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Lung cancer accounts for the vast majority of cancer-related deaths worldwide, and aberrant miRNA expression is commonly observed as the disease progresses. The current study aimed to determine the role of miR-4757-3p in the development of lung cancer. The real-time PCR test was performed to determine the expression of miR-4757-3p in lung cancer cell lines. miR-4757-3p was downregulated in A549 cells. CCK8 and transwell assays demonstrated that overexpression of miR-4757-3p significantly reduced A549 cell invasion and migration. Bioinformatic analysis by the TargetScan database predicted the possible targets of miR-4757-3p. A luciferase activity test was used to determine the direct relationship between miR-4757-3p, Wnt5a, and Wnt8b. The overexpression of miR-4757-3p drastically inhibited the expression of Wnt5a and Wnt8b. Furthermore, we discovered that silencing Wnt5a and Wnt8b significantly lowered β-catenin expression and hampered invasion and migration. Finally, miR-4757-3p inhibited lung cancer cell migration and invasion by inhibiting the activation of the Wnt signaling pathway. Our study provided evidence that miR-4757-3p could be developed as an indicator or an anticancer target in the clinical application.

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WNT8B as an Independent Prognostic Marker for Nasopharyngeal Carcinoma

Cited by 3 publications

References 60 publications

Transcriptomic profiling revealed FZD10 as a novel biomarker for nasopharyngeal carcinoma recurrence

Transcriptomic profiling revealed FZD10 as a novel biomarker for nasopharyngeal carcinoma recurrence

Precision medicine in nasopharyngeal carcinoma: comprehensive review of past, present, and future prospect

miR-4757-3p Inhibited the Migration and Invasion of Lung Cancer Cell via Targeting Wnt Signaling Pathway

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