Wnt5a regulates the cell proliferation and adipogenesis via MAPK‐independent pathway in early stage of obesity

Abstract

The early stage of obesity is an important stage in the development of obesity. However, there are few studies which explored the property or changes in obesity at early stage especially involving Wnt5a. The associated gene expression of Wnt5a on cell regeneration and the effect of Wnt5a on rat adipose-derived stem cell (rASC) proliferation and adipogenesis need additional study. Here, we investigated the changes in obesity at early stage and how Wnt5a regulates rASC regeneration, proliferation, and adipogenesis. Our data revealed that obesity at early stage measured by Lee index presented a state with impaired adipogenesis and more infiltrated inflammatory cells but without significant changes in adipocyte sizes and inflammatory factors. The process might be associated with anti-canonical Wnt pathway and a reciprocal Wnt5a/JNK pathway. Besides the gene expression of Wnt5a decreased from cell passage 1 to passage 3. The cell proliferation was regulated by increasing dose of Wnt5a with the maximal effect at 50 ng/mL and 50 ng/mL Wnt5a suppressed adipogenic differentiation at middle-late stage of adipogenesis via anti-b-catenin and a mitogen-activated protein kinase (MAPK) signaling-independent manner. Accordingly, the research helps to gain further insights into the early stage of obesity and its associated changes on a cellular and molecular level.

Diet-Induced Obesity Affects Muscle Regeneration After Murine Blunt Muscle Trauma—A Broad Spectrum Analysis

“…Wnt5a is associated with the non-canonical pathway in Wnt signaling, controlling intracellular calcium levels. Despite recent findings from Tang et al we did not find any distinct changes in the upregulation of Wnt5a which is supposed to correlate with early-onset obesity (Tang et al, 2018 ). Fuster et al described Wnt5a as a gene closely correlated with obesity-related metabolic dysfunction by promoting insulin resistance via augmentation of tissue inflammation (Fuster et al, 2015 ).…”

Pannexin 1 regulates adipose stromal cell differentiation and fat accumulation

“…Our published data indicate that knocking down Panx1 in aggressive BL6 melanoma cells results in a substantial reduction of β-catenin abundance, and decreased proliferation 49 . Similarly, in an early stage obesity rat model, a significant reduction in β-catenin levels and increased adipogenesis have been reported to be dependent on Wnt/MAPK-signaling 50 .We propose that intracellular Panx1 may control the differentiation and proliferative capacities of ASCs through modulation of calcium and/or other regulatory pathways such as Wnt signaling. This is the subject of current research efforts by our group and others.…”

Hybrid Sequencing in Different Types of Goat Skeletal Muscles Reveals Genes Regulating Muscle Development and Meat Quality

“…WNT5B activates adipocyte differentiation by promoting the expression of adipocyte markers and by relieving WNT3A-suppressed adipogenesis [50]. Conversely, WNT5A, which is closely related to WNT5B, inhibits adipogenesis in rat stromal vascular cells [51]. ADIG is a novel transcription factor involved in adipogenic differentiation.…”