Wnt/β-catenin Signaling Pathway Regulates Specific lncRNAs That Impact Dermal Fibroblasts and Skin Fibrosis

Mullin, Nathaniel K.; Mallipeddi, Nikhil V; Hamburg-Shields, Emily; Ibarra, Beatriz; Khalil, Ahmad M.; Atit, Radhika

doi:10.3389/fgene.2017.00183

Cited by 32 publications

(32 citation statements)

References 69 publications

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“…GRN is found to be up-regulated in neurodegenerative diseases such as AD and multiple sclerosis and may also function in neuro-inflammation [ 65 , 66 , 67 ]. Another lncRNA whose expression was increased by lipotoxic injury in male hippocampal microvessels was Gm12603 (WINCR1), important in collective cell migration and collagen contraction [ 68 ], suggesting that lipotoxic injury-activated WINCR1 might affect endothelial cell migration and contractibility, and also potentially endothelial permeability.…”

Section: Discussionmentioning

confidence: 99%

Lipotoxic Injury Differentially Regulates Brain Microvascular Gene Expression in Male Mice

et al. 2020

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The Western diet (WD) and hyperlipidemia are risk factors for vascular disease, dementia, and cognitive impairment. However, the molecular mechanisms are poorly understood. This pilot study investigated the genomic pathways by which the WD and hyperlipidemia regulate gene expression in brain microvessels. Five-week-old C57BL/6J wild type (WT) control and low-density lipoprotein receptor deficient (LDL-R−/−) male mice were fed the WD for eight weeks. Differential gene expression, gene networks and pathways, transcription factors, and non-protein coding RNAs were evaluated by a genome-wide microarray and bioinformatics analysis of laser-captured hippocampal microvessels. The WD resulted in the differential expression of 1972 genes. Much of the differentially expressed gene (DEG) was attributable to the differential regulation of cell signaling proteins and their transcription factors, approximately 4% was attributable to the differential expression of miRNAs, and 10% was due to other non-protein coding RNAs, primarily long non-coding RNAs (lncRNAs) and small nucleolar RNAs (snoRNAs) not previously described to be modified by the WD. Lipotoxic injury resulted in complex and multilevel molecular regulation of the hippocampal microvasculature involving transcriptional and post-transcriptional regulation and may provide a molecular basis for a better understanding of hyperlipidemia-associated dementia risk.

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Section: Discussionmentioning

confidence: 99%

Lipotoxic Injury Differentially Regulates Brain Microvascular Gene Expression in Male Mice

et al. 2020

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“…The lower number of FOXC1 + cells can disrupt the ability of the meninges to deposit laminin and maintain the BM which leads to BM defects (Zarbalis et al, ). We previously found that β‐catenin signaling transcriptionally regulates mRNA expression of extracellular matrix (ECM) genes, such as Laminin , proteoglycans, and other ECM modulating proteins in embryonic and adult dermal fibroblasts (Budnick et al, ; Hamburg‐Shields, DiNuoscio, Mullin, Lafayatis, & Atit, ; Mullins et al, ). The trophic factors originating from the non‐meningeal mesenchyme in the basolateral site appears to be β‐catenin‐dependent.…”

Section: Discussionmentioning

confidence: 99%

Wnt/β‐catenin signaling in the mouse embryonic cranial mesenchyme is required to sustain the emerging differentiated meningeal layers

DiNuoscio

Atit

2019

Genesis

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Cranial neural crest cells (CNCCs) give rise to cranial mesenchyme (CM) that differentiates into the forebrain meningeal progenitors in the basolateral and apical regions of the head. This occurs in close proximity to the other CNCC‐CM‐derivatives, such as calvarial bone and dermal progenitors. We found active Wnt signaling transduction in the forebrain meningeal progenitors in basolateral and apical populations and in the non‐meningeal CM preceding meningeal differentiation. Here, we dissect the source of Wnt ligand secretion and requirement of Wnt/β‐catenin signaling for the lineage selection and early differentiation of the forebrain meninges. We find persistent canonical Wnt/β‐catenin signal transduction in the meningeal progenitors in the absence of Wnt ligand secretion in the CM or surface ectoderm, suggesting additional sources of Wnts. Conditional mutants for Wntless and β‐catenin in the CM showed that Wnt ligand secretion and Wnt/β‐catenin signaling were dispensable for specification and proliferation of early meningeal progenitors. In the absence of β‐catenin in the CM, we found diminished laminin matrix and meningeal hypoplasia, indicating a structural and trophic role of mesenchymal β‐catenin signaling. This study shows that β‐catenin signaling is required in the CM for maintenance and organization of the differentiated meningeal layers in the basolateral and apical populations of embryonic meninges.

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“…Yuan et al (2019) Wincr1 was found to be activated by Wnt/-catenin signaling pathway in fibroblast cells. (Mullin et al, 2017) So Wincr1 might be the downstream target of Wnt signaling pathway during adipogenesis. In short, during the early adipogenesis, Wnt/-catenin signaling pathway was inhibited by the down-regulations of Kcnq1ot1, Malat1, Hotairm1 and Mir17hg and the up-regulation of Gas5 (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Wincr1, or Wnt signaling-Induced Non-Coding RNA 1, was downregulated by 0.29-fold, FDR 2.0x10-9 after 2 days of differentiation. Mullin et al (2017) reported that Wincr1 was induced by -catenin activity during skin fibrosis as its name suggests. Wincr1 further regulates the expression of key markers of dermal fibrosis.…”

Section: Identification Of Lncrnas That Significantly Changed Expressmentioning

confidence: 99%

Identification of Long Non-coding RNAs Expressed During Early Adipogenesis

Shen¹,

Soeder²,

Ophardt³

et al. 2019

OnLine Journal of Biological Sciences

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Long non-coding RNAs (lncRNAs) are transcripts with a length of more than 200 nucleotides that lack protein-coding capacity. LncRNAs play important roles in the regulation of genes during many biological processes, development and disease progression, such as obesity. Obesity is an increasing health concern around the world. Although multiple studies linking lncRNAs and fully differentiated adipocytes have been published, the systematic analysis of those lncRNAs involved in early preadipocyte differentiation, when fate determination decisions are made, has not been reported. In order to fill this gap, we conducted strandspecific RNA-Seq on mouse 3T3-L1 preadipocyte cells and compared the expression profiles before and after early differentiation for 2 days. We identified 82 lncRNAs that significantly changed their expression after early differentiation, 98% of which were newly discovered in association with early adipogenesis. The most remarkable lncRNAs were U90926, Wincr1, Kcnq1ot1, Malat1 and Hotairm1. Expression patterns for these identified genes were also highly correlated with markers of adipogenesis, including PPAR, CEBP, FABP4 and FASN. Further analysis implies that many of those altered lncRNAs might coordinately inhibit Wnt/-catenin signaling pathway to promote preadipocyte differentiation. This work strongly suggests that lncRNAs are critical in the proliferation and differentiation of adipose tissue.

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Wnt/β-catenin Signaling Pathway Regulates Specific lncRNAs That Impact Dermal Fibroblasts and Skin Fibrosis

Cited by 32 publications

References 69 publications

Lipotoxic Injury Differentially Regulates Brain Microvascular Gene Expression in Male Mice

Lipotoxic Injury Differentially Regulates Brain Microvascular Gene Expression in Male Mice

Wnt/β‐catenin signaling in the mouse embryonic cranial mesenchyme is required to sustain the emerging differentiated meningeal layers

Identification of Long Non-coding RNAs Expressed During Early Adipogenesis

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