Wnt/β-catenin signaling pathway activates melanocyte stem cells in vitro and in vivo

Guo, Haiying; Xing, Yizhan; Liu, Yingxin; Luo, Yan; Deng, Fang; Yang, Tian; Yang, Kang; Li, Yuhong

doi:10.1016/j.jdermsci.2016.04.005

Cited by 54 publications

(45 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Melanin is produced by melanosomes, large organelles in melanocytes, in a process known as melanogenesis. Wnt signaling plays a regulatory role in the melanogenesis pathway and is required for the developmental process that leads to melanocyte differentiation from neural crest cells [74,75]. One candidate lncRNA related to this process is linc-FAM204A , whose co-expressed protein-coding genes are associated with the GO terms melanogenesis, ECM-receptor interaction, and Wnt signaling pathway (Supplementary Figure S5c).…”

Section: Discussionmentioning

confidence: 99%

Non-Coding Transcriptome Maps across Twenty Tissues of the Korean Black Chicken, Yeonsan Ogye

Hong

Chai

Nam

et al. 2018

IJMS

View full text Add to dashboard Cite

Yeonsan Ogye is a rare Korean domestic chicken breed whose entire body, including feathers and skin, has a unique black coloring. Although some protein-coding genes related to this unique feature have been examined, non-coding elements have not been widely investigated. Thus, we evaluated coding and non-coding transcriptome expression and identified long non-coding RNAs functionally linked to protein-coding genes in Ogye. High-throughput RNA sequencing and DNA methylation sequencing were performed to profile the expression of 14,264 Ogye protein-coding and 6900 long non-coding RNA (lncRNA) genes and detect DNA methylation in 20 different tissues of an individual Ogye. Approximately 75% of Ogye lncRNAs and 45% of protein-coding genes showed tissue-specific expression. For some genes, tissue-specific expression levels were inversely correlated with DNA methylation levels in their promoters. Approximately 39% of tissue-specific lncRNAs displayed functional associations with proximal or distal protein-coding genes. Heat shock transcription factor 2-associated lncRNAs appeared to be functionally linked to protein-coding genes specifically expressed in black skin tissues, more syntenically conserved in mammals, and differentially expressed in black relative to in white tissues. Pending experimental validation, our findings increase the understanding of how the non-coding genome regulates unique phenotypes and can be used for future genomic breeding of chickens.

show abstract

Section: Discussionmentioning

confidence: 99%

Non-Coding Transcriptome Maps across Twenty Tissues of the Korean Black Chicken, Yeonsan Ogye

Hong

Chai

Nam

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…As crucial regulatory factors, Wnt signaling plays vital roles in the differentiation of McSCs/melanocytes in hair follicles [3, 20, 21, 23]. Activated Wnt signaling drives McSCs/melanocytes undergoing differentiation in cyclic hair follicles [3, 20, 21, 23]. However, whether the environmental factors can antagonize the increasing expression of Wnt signaling-induced differentiation of melanocytes at anagen phase needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

“…On the contrary, overexpression of Wnt1, Wnt3a, or β -catenin to the cultured mouse neural crest cells results in expansion and differentiation of melanocyte [17, 18]. Our recent work also showed that Wnt3a and Wnt10b contribute to promoting the melanogenesis of melanocytes, whereas Wnt5a inhibits the melanogenesis [19–23]. However, how the Wnt signaling is balanced to control melanocytes differentiation in the hair follicle needs further investigation.…”

Section: Introductionmentioning

confidence: 99%

Paracrine Secreted Frizzled-Related Protein 4 Inhibits Melanocytes Differentiation in Hair Follicle

Guo

Lei

et al. 2017

Stem Cells International

Self Cite

View full text Add to dashboard Cite

Wnt signaling plays crucial role in regulating melanocyte stem cells/melanocyte differentiation in the hair follicle. However, how the Wnt signaling is balanced to be overactivated to control follicular melanocytes behavior remains unknown. Here, by using immunofluorescence staining, we showed that secreted frizzled-related protein 4 (sFRP4) is preferentially expressed in the skin epidermal cells rather than in melanocytes. By overexpression of sFRP4 in skin cells in vivo and in vitro, we found that sFRP4 attenuates activation of Wnt signaling, resulting in decrease of melanocytes differentiation in the regenerating hair follicle. Our findings unveiled a new regulator that involves modulating melanocytes differentiation through a paracrine mechanism in hair follicle, supplying a hope for potential therapeutic application to treat skin pigmentation disorders.

show abstract

“…Melanin is produced by melanosomes, large organelles in melanocytes, in a process called melanogenesis. Wnt signaling has a regulatory role in the melanogenesis pathway and is also required for the developmental process that leads to melanocyte differentiation from neural crest cells 76,77 . One of the candidate lncRNAs related to the process is linc-FAM204A, whose co-expressed proteincoding genes are associated with GO terms melanogenesis, ECM-receptor interaction, and 123 million RRBS reads were analyzed (Fig.…”

Section: Discussionmentioning

confidence: 99%

HSF2 Co-regulates Protein-coding and Long Non-coding RNA Genes Specific to Black Tissues of the Black Chicken, Yeonsan Ogye

Hong

Chai

Nam

et al. 2017

Preprint

View full text Add to dashboard Cite

The Yeonsan Ogye (Ogye) is a rare Korean domestic chicken breed, the entire body of which, including its feathers and skin, has a unique black coloring. Although some protein-coding genes related to this unique feature have been examined, non-coding elements have not been globally investigated. In this study, high-throughput RNA sequencing and DNA methylation sequencing were performed to dissect the expression landscape of 14,264 Ogye protein-coding and 6900 long non-coding RNA (lncRNA) genes along with DNA methylation landscape in twenty different Ogye tissues. About 75% of Ogye lncRNAs showed tissue-specific expression whereas about 45% of protein-coding genes did. For some genes, the tissue-specific expression levels were inversely correlated with DNA methylation levels in their promoters. About 39% of the tissue-specific lncRNAs displayed functional association with proximal or distal protein-coding genes. In particular, heat shock transcription factor 2 (HSF2)-associated lncRNAs were discovered to be functionally linked to protein-coding genes that are specifically expressed in black skin tissues, tended to be more syntenically conserved in mammals, and were differentially expressed in black tissues relative to white tissues. Our results not only facilitate understanding how the non-coding genome regulates unique phenotypes but also should be of use for future genomic breeding of chickens.

show abstract

Wnt/β-catenin signaling pathway activates melanocyte stem cells in vitro and in vivo

Cited by 54 publications

References 28 publications

Non-Coding Transcriptome Maps across Twenty Tissues of the Korean Black Chicken, Yeonsan Ogye

Non-Coding Transcriptome Maps across Twenty Tissues of the Korean Black Chicken, Yeonsan Ogye

Paracrine Secreted Frizzled-Related Protein 4 Inhibits Melanocytes Differentiation in Hair Follicle

HSF2 Co-regulates Protein-coding and Long Non-coding RNA Genes Specific to Black Tissues of the Black Chicken, Yeonsan Ogye

Contact Info

Product

Resources

About