WNT/β-catenin pathway mediates the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum

Lee, Haeyong; Bae, Sung Jin; Kim, Yeong Shik; Yoon, Yoosik

doi:10.1016/j.lfs.2011.07.006

Cited by 44 publications

(32 citation statements)

References 59 publications

(68 reference statements)

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“…However, not all modulation of WNT/β-catenin pathway is accompanied by regulating Wnt10b. It is reported that IL-6 can activate the pathway with no change in the expression of Wnt10b (Lee, Bae, Kim, & Yoon, 2011). In the present study, we found that VBP had no significant influence on Wnt10b, which may be similar to the mechanism of IL-6.…”

Section: Discussionsupporting

confidence: 61%

Vanadium-binding protein from vanadium-enriched sea cucumber Apostichopus japonicus inhibits adipocyte differentiation through activating WNT/β-catenin pathway

Liu

et al. 2015

Journal of Functional Foods

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Section: Discussionsupporting

confidence: 61%

Vanadium-binding protein from vanadium-enriched sea cucumber Apostichopus japonicus inhibits adipocyte differentiation through activating WNT/β-catenin pathway

Liu

et al. 2015

Journal of Functional Foods

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“…PD exhibited anti-inflammatory, anti-allergic, cholesterol-lowering and neuroprotective properties [10,11], and exerted remarkable anti-cancer effects on different kinds of cancer cell lines, such as HepG2, MDA-MB-231, U937, K562, and THP-1 etc. , by promoting apoptosis, inducing cell cycle arrest, and inhibiting the migration and invasion of cancerous cells [12-15].…”

Section: Introductionmentioning

confidence: 99%

Platycodin D from Platycodonis Radix enhances the anti-proliferative effects of doxorubicin on breast cancer MCF-7 and MDA-MB-231 cells

Tang

Gao

et al. 2014

Chin Med

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BackgroundIt has been demonstrated that platycodin D (PD) exhibits anti-cancer activities. This study aims to investigate the anti-proliferative effects of the combination of PD and doxorubicin (DOX) on human breast cancer cells (MCF-7 and MDA-MB-231 cells).MethodsThe anti-proliferative effects of different dosages of PD, DOX, and PD + DOX on MCF-7 and MDA-MB-231 cells were determined by the MTT assay. The 10 μM PD, 5 μM DOX, and 10 μM PD + 5 μM DOX induced-protein expression of apoptosis-related molecules on MCF-7 and MDA-MB-231 cells were detected by western blot. The 10 μM PD, 5 μM DOX and 10 μM PD + 5 μM DOX-induced mitochondrial membrane potential changes on MCF-7 and MDA-MB-231 cells were stained with JC-1 before visual determination. The intracellular accumulations of DOX, induced by 10 μM PD, 5 μM DOX and 10 μM PD + 5 μM DOX, were detected by flow cytometry.ResultsPD enhanced anti-cancer activities of DOX were observed in both MCF-7 and MDA-MB-231 cell lines. Compared with mono treatment, the combined treatment increased the protein expression of cleaved poly (ADP-ribose) polymerase and decreased the mitochondrial membrane potential. The combined treatment with PD did not obviously increase the accumulation of DOX in MCF-7 cells (1.66 ± 0.13 in DOX-treated group, and 1.69 ± 0.06 in PD + DOX-treated group, P = 0.76), but it significantly increased the accumulation of DOX in MDA-MB-231 cells (1.76 ± 0.17 in DOX-treated group, 2.09 ± 0.02 in PD + DOX-treated group, P = 0.027).ConclusionThe combined treatment of DOX and PD exhibited stronger anti-proliferative effects on MCF-7 and MDA-MB-231 cells than DOX and PD treatment did.

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“…PPARγ and C/EBPα are key transcription factors in adipogenesis of 3T3-L1 cells (Lee et al, 2011). C/EBPβ is induced immediately after differentiation, whereas C/EBPα and PPARγ are expressed much later (Cristancho and Lazar, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Differentiation of 3T3-L1 preadipocytes into mature adipocytes is induced by up stimulation with three differentiation inducers (MDI); 3-isobutyl-1-methylxanthine (IBMX), dexamethasone (Dex) and insulin, which promote the accumulation of intracellular lipid droplets in mature adipocytes (Jessen and Stevens, 2002). During adipogenesis of 3T3-L1 cells, peroxisome proliferators activated receptor-γ (PPARγ) and CCAAT/enhancer-binding protein-α (C/EBPα) play key roles as major transcription factors (Lee et al, 2011). The expressions of PPAR, a transcription factor of the nuclear-receptor superfamily and C/EBPα, a member of C/EBP family basic-leucine zipper class of transcription factors are increased during differentiation of 3T3-L1 cells (Kwak et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Chrysophanic Acid Suppresses Adipogenesis and Induces Thermogenesis by Activating AMP-Activated Protein Kinase Alpha In vivo and In vitro

et al. 2016

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Chrysophanic acid (CA) is a member of the anthraquinone family abundant in rhubarb, a widely used herb for obesity treatment in Traditional Korean Medicine. Though several studies have indicated numerous features of CA, no study has yet reported the effect of CA on obesity. In this study, we tried to identify the anti-obesity effects of CA. By using 3T3-L1 adipocytes and primary cultured brown adipocytes as in vitro models, high-fat diet (HFD)-induced obese mice, and zebrafish as in vivo models, we determined the anti-obesity effects of CA. CA reduced weight gain in HFD-induced obese mice. They also decreased lipid accumulation and the expressions of adipogenesis factors including peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBPα) in 3T3-L1 adipocytes. In addition, uncoupling protein 1 (UCP1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α), the brown fat specific thermogenic genes, were up-regulated in brown adipocytes by CA treatment. Furthermore, when co-treated with Compound C, the AMP-activated protein kinase (AMPK) inhibitor, the action of CA on AMPKα was nullified in both types of adipocytes, indicating the multi-controlling effect of CA was partially via the AMPKα pathway. Given all together, these results indicate that CA can ameliorate obesity by controlling the adipogenic and thermogenic pathway at the same time. On these bases, we suggest the new potential of CA as an anti-obese pharmacotherapy.

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WNT/β-catenin pathway mediates the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum

Cited by 44 publications

References 59 publications

Vanadium-binding protein from vanadium-enriched sea cucumber Apostichopus japonicus inhibits adipocyte differentiation through activating WNT/β-catenin pathway

Vanadium-binding protein from vanadium-enriched sea cucumber Apostichopus japonicus inhibits adipocyte differentiation through activating WNT/β-catenin pathway

Platycodin D from Platycodonis Radix enhances the anti-proliferative effects of doxorubicin on breast cancer MCF-7 and MDA-MB-231 cells

Chrysophanic Acid Suppresses Adipogenesis and Induces Thermogenesis by Activating AMP-Activated Protein Kinase Alpha In vivo and In vitro

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