Wnt pathway regulation by demineralized bone is approximated by both BMP‐2 and TGF‐β1 signaling

Zhou, Shuanhu; Mizuno, Shuichi; Glowacki, Julie

doi:10.1002/jor.22244

Cited by 11 publications

(9 citation statements)

References 36 publications

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“…The TGF‐beta superfamily played many important roles in the differentiation process of the skeletal mesenchymal cells [Yamaguchi, ]. Wnt pathway regulation by demineralized bone was approximated by both BMP‐2 and TGF‐β signaling [Zhou et al, ]. Therefore, TGF‐β / BMP pathway was predicted to be involved in tooth eruption.…”

Section: Discussionmentioning

confidence: 99%

Effect and Possible Mechanism of Network Between MicroRNAs and RUNX2 Gene on Human Dental Follicle Cells

Chen

Wei

Xie

et al. 2013

J of Cellular Biochemistry

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To investigate whether crosstalk between RUNX2 and miRNAs is involved in tooth eruption regulated by dental follicle cells (DFCs) and the possible molecular mechanism. Blood samples and embedded dental follicles were collected from patients with cleidocranial dysplasia, and RUNX2 gene mutations were analyzed, then RUNX2(+/m) DFCs were isolated and identified. The characteristics of RUNX2(+/m) DFCs were analyzed. The differential expression of miRNAs was detected between the RUNX2(+/m) DFCs and RUNX2(+/+) DFCs by microarray, and target genes were predicted by miRGen. miR-146a was chosen for further investigation, and its effects in DFCs were analyzed by transfecting its mimics and inhibitors, and expression of genes involved in tooth eruption were detected. A novel insertion mutation (c.309_310insTG) of RUNX2 gene was identified which had an effect on the characteristics of DFCs. Compared with the RUNX2(+/+) DFCs, there were 69 microRNAs more than twofold up-regulated and 54 microRNAs more than twofold down-regulated in the RUNX2(+/m) DFCs. Among these, miR-146a decreased significantly in RUNX2(+/m) DFCs, and expression of RUNX2, CSF-1, EGFR, and OPG was significantly altered when miR-146a was overexpressed or inhibited. RUNX2 gene mutation contributes to the characteristic change of DFCs, and the crosstalk between RUNX2 gene and miRNAs may be one of the key regulatory mechanisms of differentiation of DFCs.

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Section: Discussionmentioning

confidence: 99%

Effect and Possible Mechanism of Network Between MicroRNAs and RUNX2 Gene on Human Dental Follicle Cells

Chen

Wei

Xie

et al. 2013

J of Cellular Biochemistry

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“…The homeostasis of collagens is maintained by the delicate balance between their synthesis and degradation via matrix metalloproteinases (MMPs). The β- catenin pathway has been linked to induction of genes encoding collagens and MMPs [ 37 – 39 ]. We next examined if collagens and MMPs were altered in mice hearts devoid of Akr1b3 post- I/R.…”

Section: Resultsmentioning

confidence: 99%

Aldose reductase modulates acute activation of mesenchymal markers via the β-catenin pathway during cardiac ischemia-reperfusion

et al. 2017

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Aldose reductase (AR: human, AKR1B1; mouse, AKR1B3), the first enzyme in the polyol pathway, plays a key role in mediating myocardial ischemia/reperfusion (I/R) injury. In earlier studies, using transgenic mice broadly expressing human AKR1B1 to human-relevant levels, mice devoid of Akr1b3, and pharmacological inhibitors of AR, we demonstrated that AR is an important component of myocardial I/R injury and that inhibition of this enzyme protects the heart from I/R injury. In this study, our objective was to investigate if AR modulates the β-catenin pathway and consequent activation of mesenchymal markers during I/R in the heart. To test this premise, we used two different experimental models: in vivo, Akr1b3 null mice and wild type C57BL/6 mice (WT) were exposed to acute occlusion of the left anterior descending coronary artery (LAD) followed by recovery for 48 hours or 28 days, and ex-vivo, WT and Akr1b3 null murine hearts were perfused using the Langendorff technique (LT) and subjected to 30 min of global (zero-flow) ischemia followed by 60 min of reperfusion. Our in vivo results reveal reduced infarct size and improved functional recovery at 48 hours in mice devoid of Akr1b3 compared to WT mice. We demonstrate that the cardioprotection observed in Akr1b3 null mice was linked to acute activation of the β-catenin pathway and consequent activation of mesenchymal markers and genes linked to fibrotic remodeling. The increased activity of the β-catenin pathway at 48 hours of recovery post-LAD was not observed at 28 days post-infarction, thus indicating that the observed increase in β-catenin activity was transient in the mice hearts devoid of Akr1b3. In ex vivo studies, inhibition of β-catenin blocked the cardioprotection observed in Akr1b3 null mice hearts. Taken together, these data indicate that AR suppresses acute activation of β-catenin and, thereby, blocks consequent induction of mesenchymal markers during early reperfusion after myocardial ischemia. Inhibition of AR might provide a therapeutic opportunity to optimize cardiac remodeling after I/R injury.

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“…Their functions vary with different tissues or developmental stages. The Wnt genes are proto-oncogenes, and the Wnt signaling pathway involves the transduction of a signal from the cell surface to the nucleus via a secreted signaling protein, a transmembrane receptor protein and various complex intracellular proteins (33).…”

Section: Bgp Alp ----------------------------------------------------mentioning

confidence: 99%

Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts

Ruan

Xue

Zong

et al. 2016

Molecular Medicine Reports

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In the present study, third‑generation autologous‑inactivated bone morphogenic protein 2 (BMP2), BMP4, BMP6, BMP7, BMP9 and Wnt3a lentiviral vectors were constructed and integrated into the genome of MC3T3‑E1 murine mesenchymal stem cells (MMSCs) to produce osteoinductive factor gene‑modified MMSCs. The transfection efficiency of each osteoinductive factor was then determined by detecting the expression levels of runt related transcription factor 2 (Runx2) mRNA. The cotransfection with combinations of two lentiviruses was performed, and the expression levels of bone γ‑carboxyglutamate protein and alkaline phosphatase in the MC3T3‑E1 cell culture supernatant were detected. The expression level of Runx2 mRNA was detected by reverse transcription‑polymerase chain reaction, and western blotting was performed to detect the protein expression levels of BMP2, BMP4, BMP6, BMP7, BMP9 and Wnt3a. The results demonstrated that the recombinant lentiviruses were successfully transfected into MC3T3‑E1 cells. The relative expression levels of Runx2 mRNA were greatest in the BMP2 group, sequentially followed by the BMP4, BMP9, BMP7, Wnt3a and BMP6 groups. The results of cotransfection of MC3T3‑E1 cells (a total of 8 groups) demonstrated that BMP‑2 and BMP‑7 exhibited the highest cotransfection efficiency. Western blot analysis demonstrated that following BMP2 and BMP7 cotransfection of MC3T3‑E1 cells, the protein expression levels of BMP2, BMP4, BMP6, BMP7, BMP9 and Wnt3a were increased compared with control cells. In conclusion, the third‑generation lentiviral vectors effectively improved the osteogenic efficiencies of MC3T3‑E1 cells, which provided an important theoretical basis and therapeutic strategy for bone reconstruction and tissue engineering.

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Wnt pathway regulation by demineralized bone is approximated by both BMP‐2 and TGF‐β1 signaling

Cited by 11 publications

References 36 publications

Effect and Possible Mechanism of Network Between MicroRNAs and RUNX2 Gene on Human Dental Follicle Cells

Effect and Possible Mechanism of Network Between MicroRNAs and RUNX2 Gene on Human Dental Follicle Cells

Aldose reductase modulates acute activation of mesenchymal markers via the β-catenin pathway during cardiac ischemia-reperfusion

Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts

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