Wnt controls the medial–lateral subdivision of the<i>Drosophila</i>head

Magri, Marta Silvia; Dominguez-Cejudo, Maria A; Casares, Fernando

doi:10.1098/rsbl.2018.0258

Cited by 10 publications

(10 citation statements)

References 24 publications

(22 reference statements)

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“…Brain tissues and proboscis were removed from the samples. All the structures were incubated overnight in Hoyer’s:Lactic Acid (1:1) solution at 80°C [32]. Imaging was carried out on a Leica DM500B microscope with a Leica DFC490 digital camera.…”

Section: Methodsmentioning

confidence: 99%

Dynamic Hh signaling can generate temporal information during tissue patterning

García-Morales

Navarro

Iannini

et al. 2018

Preprint

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The differentiation of tissues and organs requires that cells exchange information in space and time. Spatial information is often conveyed by morphogens, molecules that disperse across receiving cells generating signaling gradients. Cells translate such concentration gradients into space-dependent patterns of gene expression and cellular behavior [1, 2]. But could morphogen gradients also convey developmental time? Here, investigating the developmental role of Hh on a component of the Drosophila visual system, the ocellar retina, we discovered that ocellar cells use the non-linear gradient of Hh as a temporal cue, collectively performing the biological equivalent of a mathematical logarithmic transformation. In this way, a morphogen diffusing from a non-moving source is decoded as a wave of differentiating photoreceptors that travels at constant speed throughout the retinal epithelium.

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Section: Methodsmentioning

confidence: 99%

Dynamic Hh signaling can generate temporal information during tissue patterning

García-Morales

Navarro

Iannini

et al. 2018

Preprint

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show abstract

“…Brain tissues and proboscis were removed from the samples. All the structures were incubated overnight in Hoyer's:Lactic Acid (1:1) solution at 80°C (Magri et al, 2018). Imaging was carried out on a Leica DM500B microscope with a Leica DFC490 digital camera.…”

Section: Adult Cuticles Dissectionsmentioning

confidence: 99%

Dynamic Hh signaling can generate temporal information during tissue patterning

et al. 2019

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The differentiation of tissues and organs requires that cells exchange information in space and time. Spatial information is often conveyed by morphogens: molecules that disperse across receiving cells to generate signalling gradients. Cells translate such concentration gradients into space-dependent patterns of gene expression and cellular behaviour. But could morphogen gradients also convey developmental time? Here, by investigating the developmental role of Hh on a component of the Drosophila visual system, the ocellar retina, we have discovered that ocellar cells use the non-linear gradient of Hh as a temporal cue, collectively performing the biological equivalent of a mathematical logarithmic transformation. In this way, a morphogen diffusing from a non-moving source is decoded as a wave of differentiating photoreceptors that travels at constant speed throughout the retinal epithelium.

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“…While head horns constitute an evolutionary novelty, the patterning role of Wnt signaling discovered here may be more conserved: recent work by Magri et al shows that inhibiting Wnt signaling in the medial/dorsal head of adult Drosophila can expand the lateral compound eye into the medial/dorsal domain, resulting in a phenotype that parallels the expansion of the horn forming posterior region we have described here [31]. Further, the authors show that this lateral expansion is caused by a mechanism that relies on a gene network involving otd, eyeless, hh, and dpp, all of which function to grow and pattern the medial head domain of flies including ocelli, conserved single lensed eyes secondarily lost in almost all beetles [31,32]. Intriguingly, otd, hh, and dpp are also critical for horn specification and positioning in O. taurus [11,22,33].…”

Section: (I) Embryonic Head Patterning Genes Are Predominantly Expresmentioning

confidence: 61%

Integrating evolutionarily novel horns within the deeply conserved insect head

Linz

Moczek

2020

BMC Biol

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Background: How novel traits integrate within ancient trait complexes without compromising ancestral functions is a foundational challenge in evo-devo. The insect head represents an ancient body region patterned by a deeply conserved developmental genetic network, yet at the same time constitutes a hot spot for morphological innovation. However, the mechanisms that facilitate the repeated emergence, integration, and diversification of morphological novelties within this body region are virtually unknown. Using horned Onthophagus beetles, we investigated the mechanisms that instruct the development of the dorsal adult head and the formation and integration of head horns, one of the most elaborate classes of secondary sexual weapons in the animal kingdom. Results: Using region-specific RNAseq and gene knockdowns, we (i) show that the head is compartmentalized along multiple axes, (ii) identify striking parallels between morphological and transcriptional complexity across regions, yet (iii) fail to identify a horn-forming gene module. Instead, (iv) our results support that sex-biased regulation of a shared transcriptional repertoire underpins the formation of horned and hornless heads. Furthermore, (v) we show that embryonic head patterning genes frequently maintain expression within the dorsal head well into late post-embryonic development, thereby possibly facilitating the repurposing of such genes within novel developmental contexts. Lastly, (vi) we identify novel functions for several genes including three embryonic head patterning genes in the integration of both posterior and anterior head horns. Conclusions: Our results illuminate how the adult insect head is patterned and suggest mechanisms capable of integrating novel traits within ancient trait complexes in a sex-and species-specific manner. More generally, our work underscores how significant morphological innovation in developmental evolution need not require the recruitment of new genes, pathways, or gene networks but instead may be scaffolded by pre-existing developmental machinery.

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Wnt controls the medial–lateral subdivision of theDrosophilahead

Cited by 10 publications

References 24 publications

Dynamic Hh signaling can generate temporal information during tissue patterning

Dynamic Hh signaling can generate temporal information during tissue patterning

Dynamic Hh signaling can generate temporal information during tissue patterning

Integrating evolutionarily novel horns within the deeply conserved insect head

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