Wnt-1 signaling inhibits human umbilical vein endothelial cell proliferation and alters cell morphology

Cheng, Ching-wen; Smith, Stephen K.; Charnock-Jones, D. Stephen

doi:10.1016/j.yexcr.2003.07.006

Cited by 54 publications

(35 citation statements)

References 82 publications

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“…Expression of Wnt ligands, such as Wnt-5a, Wnt-7a, Wnt10b, and Wnt-13, has been observed in human and mouse microvascular endothelial cells in vitro (33)(34)(35). Fz-4, Fz-5, and Fz-6 are expressed in human umbilical vein endothelial cells (HUVECs) (34).…”

Section: Canonical Wnt Signaling In Vascular Endothelial Cellsmentioning

confidence: 99%

“…Fz-4, Fz-5, and Fz-6 are expressed in human umbilical vein endothelial cells (HUVECs) (34). In addition to the Wnt ligands and Fz receptors, endothelial cells express intracellular Wnt signaling molecules, such as b-catenin-associated transcription factors Tcf-1, Tcf-3, Tcf-4, and Lef-1, and respond to Wnt activation (35).…”

Section: Canonical Wnt Signaling In Vascular Endothelial Cellsmentioning

confidence: 99%

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The Wnt pathway and the roles for its antagonists, DKKS, in angiogenesis

et al. 2012

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SummaryThe Wnt signaling pathway is involved in a wide range of developmental and physiological processes, such as cell fate specification, tissue morphogenesis, and homeostasis. Thus, its dysregulation has been found in multiple diseases, including some cardiovascular disorders. The loss or gain of function of Wnt pathway components results in abnormal vascular development and angiogenesis. Further study has revealed that Wnt signaling in endothelial cells appears to contribute to vascular morphogenesis and endothelial cell specification. Owing to the significance of Wnt signaling in angiogenesis, Wnt antagonists have been considered potential treatments for neovascular disorders. In line with this, members of the Dkk protein family (Dkks), well-known Wnt antagonists, have been recently found to regulate angiogenesis. This review summarizes our present knowledge of the roles of Wnt signaling and Wnt antagonists, particularly Dkks, in angiogenic regulation and explores the therapeutic potential of Wnt antagonists.

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Section: Canonical Wnt Signaling In Vascular Endothelial Cellsmentioning

confidence: 99%

Section: Canonical Wnt Signaling In Vascular Endothelial Cellsmentioning

confidence: 99%

The Wnt pathway and the roles for its antagonists, DKKS, in angiogenesis

et al. 2012

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“…Several Fz receptors are found on the surface of primary ECs, and these cells respond to Wnt ligands stimulation (Cheng et al, 2003;Masckauchán et al, 2005;Zerlin et al, 2008). Both of Wnt1 and Wnt5a which target the canonical and noncanonical Wnt pathways can induce EC proliferation (Masckauchán et al, 2005.…”

Section: Wnt Signaling Pathway and Angiogenesismentioning

confidence: 99%

Wnt pathway antagonists and angiogenesis

Zhang

Xing

2010

Protein Cell

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Dysregulation of the Wnt pathway has been extensively studied in multiple diseases, including some angiogenic disorders. Wnt signaling activation is a major stimulator in pathological angiogenesis and thus, Wnt antagonists are believed to have therapeutic potential for neovascular disorders. Actually, some Wnt antagonists have been identified directly from the anti-angiogenic factor family. This review summarizes the recent progress toward understanding of the roles of Wnt pathway antagonists in angiogenic regulation and their mechanism of action, and exploring their therapeutic potential.

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“…Endogenous expression of Wnt ligands in human and mouse endothelial cells has been previously analyzed (Wright et al, 1999;Cheng et al, 2003), yet their role in angiogenesis is still unclear. Wnt5a transcripts were detected in human primary endothelial cells from both large vessels (HUVEC) and microvasculature (HMVEC), as evaluated by RT-PCR ( Figure 1A).…”

Section: Wnt5a Is Expressed In Isolated Human Endothelial Cells and Mmentioning

confidence: 99%

Wnt5a Signaling Induces Proliferation and Survival of Endothelial Cells In Vitro and Expression of MMP-1 and Tie-2

Masckauchán

Agalliu

Vorontchikhina

et al. 2006

MBoC

194

162

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Wnts are lipid-modified secreted glycoproteins that regulate diverse biological processes. We report that Wnt5a, which functions in noncanonical Wnt signaling, has activity on endothelial cells. Wnt5a is endogenously expressed in human primary endothelial cells and is expressed in murine vasculature at several sites in mouse embryos and tissues. Expression of exogenous Wnt5a in human endothelial cells promoted angiogenesis. Wnt5a induced noncanonical Wnt signaling in endothelial cells, as measured by Dishevelled and ERK1/2 phosphorylation, and inhibition of canonical Wnt signaling, a known property of Wnt5a. Wnt5a induced endothelial cell proliferation and enhanced cell survival under serum-deprived conditions. The Wnt5a-mediated proliferation was blocked by Frizzled-4 extracellular domain. Wnt5a expression enhanced capillary-like network formation, whereas reduction of Wnt5a expression decreased network formation. Reduced Wnt5a expression inhibited endothelial cell migration. Screening for Wnt5a-regulated genes in cultured endothelial cells identified several encoding angiogenic regulators, including matrix metalloproteinase-1, an interstitial collagenase, and Tie-2, a receptor for angiopoietins. Thus, Wnt5a acts through noncanonical Wnt signaling to promote angiogenesis.

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Wnt-1 signaling inhibits human umbilical vein endothelial cell proliferation and alters cell morphology

Cited by 54 publications

References 82 publications

The Wnt pathway and the roles for its antagonists, DKKS, in angiogenesis

The Wnt pathway and the roles for its antagonists, DKKS, in angiogenesis

Wnt pathway antagonists and angiogenesis

Wnt5a Signaling Induces Proliferation and Survival of Endothelial Cells In Vitro and Expression of MMP-1 and Tie-2

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