Wnt-1 promotes neuronal differentiation and inhibits gliogenesis in P19 cells

Involvement of the Mouse Prp19 Gene in Neuronal/Astroglial Cell Fate Decisions

“…Wnt-1 signals through a receptor complex composed of members of the Frizzled and low density lipoprotein-related protein families and activates the ␤-catenin/transcriptional T-cell factor signaling pathway (known as the canonical Wnt pathway) (52). The overexpression of Wnt-1 directs P19 cells to differentiate into neurons, but not astrocytes (25), perhaps via the enhancement of Ngn-1 gene expression (52). On the other hand, the Id-1 gene encodes an HLH factor to form a heterodimer with bHLH factors and inhibits the DNA-binding function of bHLH factors (53,54).…”

“…During the neural differentiation of aggregated P19 cells in the presence of RA, EGF receptor and BMP4 mRNA expression is markedly up-regulated (25,69). We observed that both aggregation and RA treatments were required for the astroglial cell differentiation of ␤-S1 cells, in which sense Prp19␤ RNA was overexpressed (Fig.…”

“…The primer sequences used were as follows: mouse Prp19␣ and Prp19␤, 5Ј-CGC CAT GTC CCT GAT CTG CTC G-3Ј (5Ј-primer) and 5Ј-GAT GCC ACC TGC CGG TAC TTG C-3Ј) (3Ј-primer); glyceraldehyde-3-phosphate dehydrogenase, 5Ј-CCA CAG TCC ATG CCA TCA CTG CC-3Ј (5Ј-primer) and 5Ј-GGC AGT GAT GGC ATG GAC TGT GG-3Ј (3Ј-primer) (19); ribosomal phosphoprotein, 5Ј-CAG CTC TGG AGA AAC TGC TG-3Ј (5Ј-primer) and 5Ј-GTG TAC TCA GTC TCC ACA GA-3Ј (3Ј-primer) (20); oct-3/4, 5Ј-CCT GGC TAA GCT TCC AAG GGC-3Ј (5Ј-primer) and 5Ј-GTT CTA GCT CCT TCT GCA GGG C-3Ј (3Ј-primer) (21); nanog, 5Ј-CTA TGA TCT TTC CTT CTA GAC ACT G-3Ј (5Ј-primer) and 5Ј-AGC CCA AAG CTT GCG TAA GTC TCA TAT T-3Ј (3Ј-primer) (22,23); Mash-1, 5Ј-CAC AAG TCA GCG GCC AAG CAG-3Ј (5Ј-primer) and 5Ј-GAT CCC TCG TCG GAG GAG TAG-3Ј (3Ј-primer) (24); Ngn-1, 5Ј-CCT TTG GAG ACC TGC ATC TC-3Ј (5Ј-primer) and 5Ј-GAT GTA GTT GTA GGC GAA GC-3Ј (3Ј-primer) (25); NeuroD, 5Ј-GCA TGC ACG GGC TGA ACG C-3Ј (5Ј-primer) and 5Ј-GGG ATG CAC CGG GAA GGA AG-3Ј (3Ј-primer) (26); GFAP, 5Ј-TTT CTC CTT GTC TCG AAT GA-3Ј (5Ј-primer) and 5Ј-GGT TTC ATC TTG GAG CTT CT-3Ј (3Ј-primer) (25); otx2, 5Ј-CCG ACT TTG CGC CTC CAA ACA A-3Ј (5Ј-primer) and 5Ј-GGT TGA TGG ACC CTT CTA AGG C-3Ј (3Ј-primer) (27); retinoic acid receptor (RAR)␣, 5Ј-CAT CAC AAC TAC CTG CCA GAC TC-3Ј (5Ј-primer) and 5Ј-GTG CGC TTT GCG AAC CTT CTC AA-3Ј (3Ј-primer) (28); RAR␤, 5Ј-CGC CTC CAC ACC TAG AGG ATA AG-3Ј (5Ј-primer) and 5Ј-TGG GGA ATG TCT GCA ACA GCT GG-3Ј (3Ј-primer) (29); and CypA, 5Ј-CGC GAA TTC CAG CCA TGG TCA ACC CCA C-3Ј (5Ј-primer) and 5Ј-CCA CTC GAG TTA GAG CTG TCC ACA GTC G-3Ј (3Ј-primer) (30).…”

Threshold in Stage-specific Embryonic Glycotypes Uncovered by a Full Portrait of Dynamic N-Glycan Expression during Cell Differentiation

“…All experiments were performed in triplicate and repeated twice independently (n ϭ 6). Mouse ESCs were differentiated under conditions reported previously (30,31). Basic Protocol of Glycoblotting-based Quantitative Cellular N-Glycomics-A preliminary trial for the cellular N-glycomics was reported previously in human prostate cancer cells (PC-3) and normal human prostate epithelial cells on the basis of the protocol designed for human serum N-glycomics using the BlotGlyco TM ABC bead, a prototype bead prepared by conjugating N-(2-aminobenzoyl)cysteine hydrazide and thiopropyl-Sepharose 6B (26).…”

Stress in the brain: novel cellular mechanisms of injury linked to Alzheimer's disease

“…As one of the best characterized members of the Wnt family, Wnt-1 was first identified as a proto-oncogene in mammary carcinomas, but has recently been illustrated to play a critical role in neuronal development [216]. Wnt functions by binding to the transmembrane receptor Frizzled and the co-receptor lipoprotein related protein 5 and 6 (LRP-5/6) [244] followed by recruitment of dishevelled, the cytoplasmic bridging molecule, to inhibit glycogen synthase kinase (GSK-3β) [93,170].…”