Within-day Physiologic Variation of Leukocyte Types in Healthy Subjects as Assayed by Two Automated Leukocyte Differential Analyzers

Abstract

The physiologic within-day (700-2200 h) variation of leukocyte-type concentrations in blood as determined for 21 healthy young adults is reported. All blood specimens were obtained in duplicate such that the within-batch analytic variation, as well as the pertinent biologic sources of variation, was able to be determined. All specimens were analyzed on each of two automated leukocyte differential systems: Hemalog-D Differential System and the Hematrak-240 Analyzer. On the basis of a comparison of the performances of the two analyzers, it was decided to report the neutrophil and the lymphocyte values as measured on both systems, the monocyte values as measured on teh Hematrak only, and the eosinophil and basophil values as measured on the Hemalog-D only. The intrasubject within-day physiologic variations for the cell on the Hemalog-D and Hematrak, respectively, in terms of coefficient of variation wee as follows: neutrophils 19.4% and 19.6%; lymphocytes, 13.8% and 17.5%. For monocytes, as measured on the Hematrak, it was 13.4%. For eosinophils and basophils, as measured on the Hemalog-D, it was 27.2% and 8.3%, respectively. There was a consistent group-specific diurnal variance that amounted to more than 40% of the total within-day variance, both for lymphocytes and for eosinophils. The within-day physiologic variation of the cell type concentrations for eight of the volunteers was compared with that of the plasma cortisol concentrations, as determined on specimens derived from the same veni-puncture sessions. The decrease in plasma cortisol values was in most instances associated with decreases in eosinophils and increases in neutrophils. For total leukocytes and neutrophils, the mean concentration for smokers was significantly higher than that for nonsmokers.

Social regulation of leukocyte homeostasis: The role of glucocorticoid sensitivity

“…The utility of hematologic biomarkers of glucocorticoid sensitivity might be substantially improved by intra-individual assessments over time (e.g., repeated measurements of cortisol and leukocyte subset distributions over their diurnal cycle (Winkel, Statland et al 1981)). That would hold constant other hematologic influences that likely added substantial noise to the present cross-sectional analysis (and likely explain why relationships between cortisol levels and hematologic parameters rarely exceeded r = .20 in magnitude).…”

Haematological and Biochemical Parameters in an Industrial Workforce

“…It is known to fall markedly between birth and age 21 but further information is sparse. In our survey, the values for men rose steadily.…”

Biological Variations of Hematologic Parameters Determined by UniCel DxH 800 Hematology Analyzer

“…1 Several previous studies have investigated the biological variations of hematologic parameters. [2][3][4][5][6][7][8][9] Some parameters, such as hemoglobin or reticulocytes, have been demonstrated to exhibit hour-to-hour, day-to-day, or seasonal intraindividual fluctuation. 3,6 We revisit these areas for several reasons.…”

“…Second, almost all the reported studies in the past have been based entirely on Caucasian/white populations. [2][3][4][5][6][7][8][9] There is little knowledge about biological variation of hematologic parameters among Asian populations. Third, many new hematologic parameters have become available from modern automated hematology analyzers in recent years.…”