Withdrawn: The LncRNA H19/microRNA‐29b‐3p/HMGB1 signaling axis contributes to the regulation of lung cancer cell growth

Abstract

The long non-coding RNA (lncRNA) H19 acts as a competitive endogenous RNA (ceRNA) of miR-29b-3p and has been reported to exert pro-tumorigenic roles in several cancer types.However, the role of lncRNA H19 in lung cancer is not fully understood. Here, we investigated the role of the lncRNA H19/microRNA-29b-3p (miR-29b-3p)/high mobility group box 1 (HMGB1) signaling pathway in lung cancer cell growth using 293T, NCI-H1975, Calu-3 and 2BS cell lines. Cell viability was determined using a cell counting kit-8 (CCK-8) assay, while apoptosis and cell cycle distribution were assessed by flow cytometry. Cell migration was detected using a wound healing assay. Cell invasion was evaluated by transwell assay. The expression of lncRNA H19, miR-29b-3p, HMGB1, toll-like receptor 4 (TLR4), and matrix metallopeptidase 9 (MMP-9) was measured by fluorescence quantitative PCR or western blotting. We demonstrated that miR-29b-3p could directly bind to both lncRNA H19 and HMGB1 by dual-luciferase reporter assay. Three shRNAs targeting lncRNA H19 (shlncRNA H19) were designed, and shlncRNA H19-2 was selected to investigate the function of lncRNA H19 in tumor cell biology. Compared with controls, lung cancer cells expressing shlncRNA H19 exhibited decreased proliferation, cell-cycle arrest at the G1 phase, increased levels of cell apoptosis, and reduced migration and invasion. Moreover, shlncRNA H19 upregulated the expression of miR-29b-3p and reduced the protein expression of HMGB1, TLR4, and MMP-9 in lung cancer cells. Together, our data indicate that the lncRNA H19/miRNA-29b-3p/HMGB1 signaling axis is involved in the regulation of lung cancer cell growth.

Aloin Induces Gastric Cancer Cell Apoptosis via the miR-5683/HMGB1 Signal Axis

“…6,[12][13][14] In addition, several miRNAs play an important role in cancer pathogenesis by regulating HMGB1 expression. [15][16][17] Bioinformatics analysis has revealed that miR-5683 and HMGB1 are abnormally expressed in GC tissue. Moreover, TargetScan database has predicted that HMGB1 is the target gene for miR-5683.…”

Long Noncoding RNA H19: A Novel Therapeutic Target Emerging in Oncology Via Regulating Oncogenic Signaling Pathways

“…MiR-675, one of the targeted miRNAs of H19, is embedded in H19’s first exon, and H19 has been shown to inhibit the growth of the placenta before birth by regulating the processing of miR-675. H19 can also interact with other miRNAs, such as miR-326 ( Wei et al, 2019 ), miR-29a ( Cheng et al, 2019 ), miR-124-3p ( Liu et al, 2019 ), miR-152-3p ( Zheng et al, 2020 ), miR-22-3p ( Gan et al, 2019 ), miR-29b-3p ( Zhong et al, 2021 ), miR-193a-3p ( Ma et al, 2018 ), miR-612 ( Yu et al, 2020 ), to regulate biological processes in various types of cells by modulating the expression of downstream target factors, including twist family bHLH transcription factor 1 (TWIST1), thymine DNA glycosylase, integrin β3 (ITGB3), bromodomain containing protein 4 (BRD4), Snail1, high mobility group box 1 (HMGB1), presenilin-1 (PSEN1), and Bcl-2. Secondly, H19 is involved in regulating different processes in various types of cells by interacting with different proteins, such as methyl-CpG-binding domain protein 1 (MBD1) ( Monnier et al, 2013 ; Zhang et al, 2017 ), polypyrimidine tract-binding protein 1 (PTBP1) ( Liu et al, 2018 ), enhancer of zeste homolog 2 (EZH2) ( Luo et al, 2013 ), IGF2 mRNA-binding protein 1 (IGF2BP1) ( Runge et al, 2000 ), p53 ( Yang et al, 2012 ), K homology-type splicing regulatory protein (KSRP) ( Giovarelli et al, 2014 ), zinc finger E-box-binding homeobox 1 (ZEB1) ( Song et al, 2017 ).…”