WITHDRAWN: Mechanism of action of levocarnitine for the treatment of spermatogenic dysfunction in rats and spermatogonia

Wang, Jisheng; Bao, Bocheng; Meng, Fanchao; Deng, Sheng; Dai, Hengheng; Feng, Junlong; Li, Haisong; Wang, Bin

doi:10.21203/rs.3.rs-201302/v1

Cited by 1 publication

(1 citation statement)

References 23 publications

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“…Frontiers in Molecular Biosciences frontiersin.org and psychological factors (La et al, 2021). Cuscuta in the form of herbal mixtures has been known as a cure to many diseases, such as oligospermia (Wang et al, 2022), osteoporosis (Huiwen et al, 2021), premature ovarian failure (Jinying et al, 2020), sleep induction (Forouzanfar et al, 2020), habitual abortion (Wang et al, 2021b), and neurasthenia (Lu, 2016). In this study, 10 active ingredients (including flavonoids, glycosides, alkaloids, and steroids) from SC play an important role in the treatment of RSA.…”

Section: Discussionmentioning

confidence: 99%

Mechanisms underlying the therapeutic effects of Semen cuscutae in treating recurrent spontaneous abortion based on network pharmacology and molecular docking

Zheng,

Lei,

Yao

et al. 2024

Front. Mol. Biosci.

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Background: This paper aims to analyse the active components of Semen cuscutae (SC) by network pharmacology and screen the most stable compounds with tumour necrosis factor-alpha (TNF-α) by molecular docking to explore the mechanisms of SC treatment of recurrent spontaneous abortion (RSA) and provide a theoretical basis for drug development.Methods: The active compounds of SC and the potential inflammatory targets of RSA were obtained from the Traditional Chinese Medicine Systems Pharmacology database and GeneCards, respectively. The RSA-SC target gene interaction network was obtained and visualized using the STRING database and Cytoscape software. GO and KEGG pathway enrichment analyses were obtained from DAVID to further explore the RSA mechanism and therapeutic effects of SC. Interactions between TNF-α and drugs were analysed by molecular docking. Treatment of human trophoblast cells with sesamin and TNF-α was carried out to detect their proliferative and apoptotic abilities, and WB assay was carried out to detect EGFR, PTGS2, and CASP3 protein expression.Results: Ten compounds and 128 target genes were screened from SC, of which 79 overlapped with RSA target inflammatory genes, which were considered potential therapeutic targets. Network pharmacological analysis showed that sesamin, matrine, matrol, and other SC compounds had a good correlation with the inflammatory target genes of RSA. Related genes included PGR, PTGS1, PTGS2, TGFB1, and CHRNA7. Several signalling pathways are involved in the pathogenesis of RSA, such as the TNF-α signalling pathway, HIF-1 signalling pathway, oestrogen signalling pathway, proteoglycans in cancer cells, and FoxO signalling pathway. Molecular docking results suggested that sesamin was the most suitable natural tumour necrosis factor inhibitor (TNFi). Sesamin can promote proliferation and inhibit apoptosis in human trophoblasts by downregulating EGFR and CASP3 expression and upregulating PTGS2 expression.Conclusion: Our findings play an important role and basis for further research into the molecular mechanism of SC treatment of RSA and drug development of TNFi.

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