WITHDRAWN: LncRNA NEAT1 regulates epithelial-mesenchymal transition of osteosarcoma by miR-186-5p as competing endogenous RNA

Abstract

Increasing evidence showed that lncRNA NEAT1 play important roles in regulating tumor carcinogenesis and progression. However, the function and underlying mechanism of NEAT1 in osteosarcoma (OS) remain unclear. In the present study, we found that NEAT1 expression was significantly upregulated in OS tissues and cell lines. High NEAT1 expression was closely associated with advanced clinicopathologic features and poor overall survival of OS patients. In vitro function assay, we found that NEAT1 could promote OS cells proliferation, invasion, and epithelial-mesenchymal transition (EMT) process. NEAT1 could also promote OS cells growth in vivo. In addition, our studies showed that miR-186-5p was a downstream target of NEAT1 in OS. Functionally, miR-186-5p suppressed OS cells proliferation, invasion, and EMT process. Furthermore, our data revealed that HIF-1α was a downstream target of miR-186-5p and NEAT1 could exert its tumor oncogenic roles on OS cells via miR-186-5p/ HIF-1α axis. Taken together, we elucidated that the NEAT1/miR-186-5p/HIF-1α axis might be a therapeutic approach for the treatment of OS.