WITHDRAWN: Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using integration free Sendai technology

Abstract

The Publisher regrets that this article is an accidental duplication of an article that has already been published in Stem Cell Res., 16 (2016) 133–136, http://dx.doi.org/10.1016/j.scr.2015.12.021. The duplicate article has therefore been withdrawn. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy.

Human neural progenitors derived from integration-free iPSCs for SCI therapy

“…To avoid the side effects, non-integrating protocols using episomal vectors, Cre-lox system, piggybac vectors, minicircles, recombinant proteins, messenger RNAs, microRNAs, and small molecules, have recently been reported ( Chang et al, 2009 ; Kaji et al, 2009 ; Kim et al, 2009 ; Sommer et al, 2009 ; Woltjen et al, 2009 ; Yu et al, 2009 ; Zhou et al, 2009 ; Jia et al, 2010 ; Warren et al, 2010 ; Anokye-Danso et al, 2011 ; Rao and Malik, 2012 ; Hou et al, 2013 ), which have shown variable yields and reproducibility. Recently, Sendai viruses have been established and shown to be able to reprogram dermal fibroblasts, CD34+ hematopoietic cells and urine derived cells ( Fusaki et al, 2009 ; Ye et al, 2013 ; Afzal and Strande, 2015 ; Rossbach et al, 2016 ). As negative sense RNA viruses, Sendai viruses do not integrate into the genome of human cells and are non-pathogenic to humans ( Fusaki et al, 2009 ; Ban et al, 2011 ; Macarthur et al, 2012a ).…”

“…Several somatic cell types have been commonly used for iPSC reprogramming such as fibroblasts or keratinocytes from skin biopsies, lymphocytes and CD34+ hematopoietic stem cells harvested from blood ( Ye et al, 2009 ; Mack et al, 2011 ; Ye et al, 2013 ). Recently, cells derived from urine were reported to be able to be reprogrammed into iPSCs ( Zhou et al, 2012 ; Wang et al, 2013a ; Guan et al, 2014 ; Afzal and Strande, 2015 ; Rossbach et al, 2016 ). Urine can be easily obtained from patients via non-invasive procedures and only 100 mL of urine is sufficient for isolation, culture, and subsequent reprogramming.…”