WITHDRAWN: Evaluation of antidiabetic effect of embelin from Embelia ribes in alloxan induced diabetes in rats

Sekar, Mahendran; Badami, Shrishailappa; Maithili, V.

doi:10.1016/j.biopha.2010.08.003

Cited by 10 publications

(5 citation statements)

References 25 publications

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“…Oral administration of extract significantly reversed all these changes. Thus, the normalization of lipids in diabetic rats treated with extract may be due to its stimulatory effect on insulin secretion from pancreatic â cells (23) .…”

Section: Resultsmentioning

confidence: 99%

Antihyperglycemic and Antihyperlipidemic Effects of Fermented Rhynchosia nulubilis in Alloxan-induced Diabetic Rats

Kim¹,

Ha²

2013

Toxicological Research

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Alloxan administration in rats is used as a model for non-insulin dependent diabetes mellitus (NIDDM). NIDDM is a multifactorial disease, characterized by hyperglycemia and lipoprotein abnormalities. In this study, we evaluated the antihyperglycemic and antihyperlipidemic effects of fermented Rhynchosia nulubilis (FRN) through the regulation of glucose uptake in alloxan-induced rats. Fermented R. nulubilis was administered orally for 28 d at 500 mg/kg of body weight. Body weight and food intake were monitored every day. Biochemical parameters were quantified after 4 week. In the diabetic + FRN group, body weight increased significantly and blood glucose concentrations decreased when compared to those of the diabetic group. After 2 hr of administration, the oral glucose tolerance test (OGTT) indicated a significant reduction in the diabetic + FRN group compared to diabetic group. The diabetic + FRN group experienced a significant reduction in total cholesterol, triglycerides, low density lipoprotein, coronary risk factors, and malondialdehyde concentrations, with significantly increased high density lipoprotein compared to those of diabetic group. These results demonstrate that fermented R. nulubilis possesses potent antihyperglycemic and antihyperlipidemic activity in alloxan-induced diabetic rats.

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Section: Resultsmentioning

confidence: 99%

Antihyperglycemic and Antihyperlipidemic Effects of Fermented Rhynchosia nulubilis in Alloxan-induced Diabetic Rats

Kim¹,

Ha²

2013

Toxicological Research

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“…Once they had adapted to the environment, 10 rats were randomly selected as the normal control group (NC); these were fed with an NPD, while the others were fed an HFD [from National Institute of Nutrition (NIN); composition of HFD is shown in l " Table 1] for 1-28 days of the experimental period, and randomly divided into 3 groups (each containing 10 rats) as follows: model control group (HFD-fed), positive control group (HFD + ORL; orlistat at 10 mg/kg from Ranbaxy Laboratories Ltd., dissolved in 0.9% NaCl), and embelin treatment group (HFD + EMB; 50 mg/kg suspended in 1 % gum acacia in 0.9 % NaCl). This dose was selected on the basis of previous reports on embelin where a 50 mg/kg dose of embelin produced significant results compared to a 25 mg/kg dose in rats [8,14,15]. The drugs were administered orally via a standard orogastric cannula everyday for the period of 8-28 days.…”

Section: High-fat Diet-induced Obesitymentioning

confidence: 99%

“…In the Indian system of medicine, "Ayurveda", the plant is popularly known as vidanga and it is an ingredient in many Ayurvedic preparations. Embelin is reported to possess antitumor, antibacterial, antioxidant, analgesic, antifertility, wound healing, anticonvulsant, and antidiabetic activities [8]. Bhandari et al [9] reported the potential of E. ribes ethanol extract in diabetic dyslipidemia and protection from lipid peroxidation in tissues in streptozotocin-induced diabetes in rats.…”

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confidence: 99%

“…Embelin at a 50 mg/kg dose showed better antihyperglycemic, lipid lowering, and antioxidant activities than a 25 mg/kg dose for alloxan-induced diabetes in rats [8]. Embelin is reported to have a high therapeutic index (LD 50 as high as 2000 mg/kg body weight in rats and mice), conferring a wide margin of safety for it [8]. Several animal models have been used to screen the pathogenesis of obesity and associated complications.…”

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confidence: 99%

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Preventive Effect of Embelin fromEmbelia ribeson Lipid Metabolism and Oxidative Stress in High-Fat Diet-Induced Obesity in Rats

2012

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A high-fat diet (HFD) results in hyperlipidemia and an increase in oxidative stress. The purpose of this study was to investigate the preventive effect of embelin against hyperlipidemia and oxidative stress in HFD-induced obesity in rats. Male Wistar rats aged 12 weeks (150-200 g) were fed with an HFD for a period of 28 days to induce experimental obesity. HFD-induced obese rats were treated with embelin (50 mg/kg) or orlistat (10 mg/kg) for 21 days. A range of parameters were tested including body weight gain, body mass index (BMI), blood pressure, visceral fat pad weights, serum levels of glucose, insulin, leptin, apolipoprotein B (ApoB), total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), hepatic thiobarbituric acid-reactive substances (TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH). Twenty-one days of embelin (50 mg/kg) treatment produced effects similar to orlistat in reducing body weight gain, blood pressure, visceral fat pad weight, serum lipid levels, as well as coronary artery risk and atherogenic indices of HFD-fed rats. Embelin treatment also lowered the serum levels of glucose by 24.77 %, insulin by 35.03 %, and leptin by 43.39 %. Furthermore, embelin treatment significantly (p < 0.01) decreased the hepatic TBARS levels, while increasing the SOD, CAT, and GSH levels in obese rats. The present study indicated the preventive effect of embelin in HFD-induced obesity and its related complications. Embelin could be valuable in the development of new drug therapies to prevent obesity, hyperlipidemia, and oxidative stress.

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“…First, the effect of dietary embelin on growth performance and diarrhea was measured. The median lethal dose of embelin is reported to be 2000 mg/kg body weight in mice and rats without any mortality or adverse effects indicating its safety [27]. So, we chose 200, 400 and 600 mg/kg embelin as the additive dosage.…”

Section: Discussionmentioning

confidence: 99%

Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells

Yao

Huang

Bao

et al. 2022

J Animal Sci Biotechnol

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Background Intestinal barrier plays key roles in maintaining intestinal homeostasis. Inflammation damage can severely destroy the intestinal integrity of mammals. This study was conducted to investigate the protective effects of embelin and its molecular mechanisms on intestinal inflammation in a porcine model. One hundred sixty 21-day-old castrated weaned pigs (Duroc × Landrace × Yorkshire, average initial body weight was 7.05 ± 0.28 kg, equal numbers of castrated males and females) were allotted to four groups and fed with a basal diet or a basal diet containing 200, 400, or 600 mg embelin/kg for 28 d. The growth performance, intestinal inflammatory cytokines, morphology of jejunum and ileum, tight junctions in the intestinal mucosa of piglets were tested. IPEC-1 cells with overexpression of P300/CBP associating factor (PCAF) were treated with embelin, the activity of PCAF and acetylation of nuclear factor-κB (NF-κB) were analyzed to determine the effect of embelin on PCAF/NF-κB pathway in vitro. Results The results showed that embelin decreased (P < 0.05) serum D-lactate and diamine oxidase (DAO) levels, and enhanced the expression of ZO-1, occludin and claudin-1 protein in jejunum and ileum. Moreover, the expression levels of critical inflammation molecules (interleukin-1β, interleukin-6, tumor necrosis factor-α, and NF-κB) were down-regulated (P < 0.05) by embelin in jejunal and ileal mucosa. Meanwhile, the activity of PCAF were down-regulated (P < 0.05) by embelin. Importantly, transfection of PCAF siRNAs to IPEC-1 cell decreased NF-κB activities; embelin treatment downregulated (P < 0.05) the acetylation and activities of NF-κB by 31.7%–74.6% in IPEC-1 cells with overexpression of PCAF. Conclusions These results suggested that embelin ameliorates intestinal inflammation in weaned pigs, which might be mediated by suppressing the PCAF/NF-κB signaling pathway.

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WITHDRAWN: Evaluation of antidiabetic effect of embelin from Embelia ribes in alloxan induced diabetes in rats

Cited by 10 publications

References 25 publications

Antihyperglycemic and Antihyperlipidemic Effects of Fermented Rhynchosia nulubilis in Alloxan-induced Diabetic Rats

Antihyperglycemic and Antihyperlipidemic Effects of Fermented Rhynchosia nulubilis in Alloxan-induced Diabetic Rats

Preventive Effect of Embelin fromEmbelia ribeson Lipid Metabolism and Oxidative Stress in High-Fat Diet-Induced Obesity in Rats

Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells

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