WITHDRAWN: Dysregulation of miR-129-5p in Patients with Non-Alcoholic Fatty Liver Disease and Its Regulatory Effect on Insulin Resistance

Abstract

Background: Non-alcoholic fatty liver (NAFLD) is the hepatic manifestation of metabolic syndromes, insulin resistance (IR) is related to the occurrence of NAFLD. The purpose of this study was to evaluate the expression of miR-129-5p in NAFLD patients and its clinical value, and explore its regulatory effect on IR.Methods: 117 NAFLD patients and 110 healthy controls were included. The levels of miR-129-5p were detected by qRT-PCR. To assess the diagnostic value of miR-129-5p for NAFLD, the receiver operating characteristic curve (ROC) was established. C57Bl/6 mice were supplied with high-fat diet to establish NAFLD model. Intraperitoneal insulin tolerance test (IPITT) was carried out to evaluate the effect of miR-129-5p on IR in NAFLD animal model.Results: miR-129-5p was highly expressed in the serum of NAFLD patients, and patients with HOMA-IR ≥ 2.5 had higher level of miR-129-5p than those with HOMA-IR < 2.5. miR-129-5p had the ability to differentiate NAFLD patients from healthy individuals, and might be associated with the development of IR. Serum miR-129-5p was positively correlated with the levels of HOMA-IR, BMI, total cholesterol (TC) and triglyceride (TG) in NAFLD patients. Downregulation of miR-129-5p regulates lipid metabolism and insulin sensitivity in NAFLD mice model.Conclusions: MiR-129-5p was up-regulated in NAFLD patients, and might be a potential diagnostic biomarker. The regulatory effect of miR-129-5p on NAFLD may function by regulating lipid accumulation and insulin sensitivity.