Withania somnifera- A Boom for Cancer Patients

Singh, Ravideep; Sharma, Pooja; Kumar, Dinesh

doi:10.35652/igjps.2019.92s24

Cited by 3 publications

(3 citation statements)

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“…Studies have shown that Plant-derived compounds have been an important source of several clinically useful anti-cancer agents (Cragg and Newman, 2005;Yesil-Celiktas et al, 2010;Singh et al, 2011;Bronikowska et al, 2012). The findings also indicated that cytotoxic effect of…”

Section: Introductionmentioning

confidence: 93%

Anti-proliferative effects of Rhamnus frangula miller leaf and bark extracts on HEK293 and MCF-7 cell lines and evaluation of Bax and Bcl-2 genes expression level in MCF-7 cells

Moulavi1Pooria Moulavi,

Saberi,

Najafi

et al. 2022

JBS

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Rhamnus frangula extracts may have anti-proliferative effects on cancer development. This study was exerted to determine the effects of Rhamnus frangula Miller leaf and bark extracts on the viability of MCF-7 cells compared with HEK293 cells and the evaluation of Bax and Bcl-2 gene expression levels in MCF-7 cells. The cell lines were randomly divided into the control group (not exposed to extract) and groups exposed to 0.001, 0.01, 0.1, and 1 mg/ml of Rhamnus frangula leaf and bark extracts. Cell viability was quantified by MTT assay. The expression of Bax and Bcl-2 genes was evaluated by quantitative Real-time PCR analysis. Statistical analysis was performed using ANOVA. HEK293 cells viability significantly increased in groups exposed to 0.001 and 0.01 mg/ml and decreased in group exposed to 1 mg/ml of Rhamnus frangula leaf extract compared to control group (P < 0.01, P < 0.05 and P < 0.01, respectively). Exposure of MCF-7 cells to 0.01, 0.1 and 1 mg/ml of leaf extract led to significant decrease in cell viability (P < 0.05). Exposure of HEK293 cells to 1 mg/ml and of MCF-7 cells to 0.1 and 1 mg/ml of Rhamnus frangula bark extract resulted in significant decrease in cell viability compared to control group (P < 0.01, P < 0.05 and P < 0.01, respectively). The cytotoxic effect of leaf was higher than bark extract on MCF7 cells. 1 mg/ml of leaf and bark extracts significantly increased the expression level of pro-apoptotic Bax and decreased anti-apoptotic Bcl-2 genes in MCF-7 cells (P < 0.01). However, the expression level of the Bax gene was significantly higher in a group exposed to 1 mg/ ml of leaf compared to bark extract (P < 0.01). Despite bark, lower leaf extract concentrations inhibit breast cancer (MCF-7) cells proliferation while having no cytotoxic effects on non-cancerous HEK293 cells. Therefore, using leaf is more appropriate than bark extract to fight against breast cancer cells. We have also shown that Rhamnus frangula leaf and bark extracts mechanism of action has occurred through Bax-dependent apoptotic pathway on breast cancer cell line.

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Section: Introductionmentioning

confidence: 93%

Anti-proliferative effects of Rhamnus frangula miller leaf and bark extracts on HEK293 and MCF-7 cell lines and evaluation of Bax and Bcl-2 genes expression level in MCF-7 cells

Moulavi1Pooria Moulavi,

Saberi,

Najafi

et al. 2022

JBS

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“…In Sanskrit, ashwagandha, the Indian name for Withania somnifera, means "odor of the horse", probably originating from the odor of its root which resembles that of a sweaty horse. The herb is termed as "Rasayana" in Ayurvedic practice, which means it acts as a tonic for vitality and longevity [6]. "Withania somnifera has been known for its potent antioxidant and free radical quenching properties in various conditions.…”

Section: World Health Organizationmentioning

confidence: 99%

Assessment of Antioxidant Activity of Withania somnifera in Equine

Yadav¹,

Meena²,

Tanwar

et al. 2023

JSRR

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Aims: The present research was done to evaluate the antioxidant activity of aqueous extract of Withania somnifera (Ashwagandha) in equine. Herbal antioxidants have gained popularity due to their potency and safety, and oxidative stress is a condition that is more prevalent in horses performing physical activities. Methods: Twenty clinically healthy horses were selected and equally divided into treatment and control group consisting of 10 animals in each. An aqueous extract was prepared from dried root powder of Withania somnifera (Ashwagandha) and was continuously administered orally for 15 days to treatment group animals @ 100 mg/kg body weight. Blood samples were collected on 0 th, 8th, and 16th day from both the treatment and control group. The plasma was used for the determination of enzymatic oxidants (Catalase (CAT) and superoxide dismutase (SOD) and non-enzymatic antioxidants (reduced glutathione (GSH) and Vitamin C). Results: A non-significant increase in the values of Catalase, Superoxide Dismutase (SOD), Reduced Glutathione (GSH), and Vitamin C were observed after administration of aqueous extract of Withania somnifera. Conclusion: Administration of aqueous extract of Withania somnifera not significantly modulated the activity of enzymatic and non-enzymatic antioxidants.

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“…According to Bhattacharya and Muruganandam [4], it possesses anti-stress qualities. Cytotoxic, antimicrobial, antifungal, immunosuppressive, and immunomodulatory qualities Singh [5]. As a sedative, for rheumatoid arthritis, for joint inflammation, for feminine diseases, for hiccups, against colds and coughs, for ulcers, as leprosy, etc.…”

Section: Introductionmentioning

confidence: 99%

Genetic Divergence of Ashwagandha (Withania somnifera (L.) Dunal) for Growth and Yield Traits

Abdulrazaq,

Gopinath,

Suneetha, C.

et al. 2024

JSRR

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Genetic divergence among 32 Ashwagandha (Withania somnifera (L.) Dunal) accessions of different geographic origins was assessed using Mahalanobis D2 statistics. Observations revealed significant genotypic differences, and accordingly, genotypes were classified into six clusters. Cluster I was the largest, with twenty genotypes, followed by the cluster II, which has eight, and clusters III, IV, V, and VI contained only one most divergent genotype. The maximum divergence was observed between clusters II and III (D2 = 47.90), followed by clusters V and VI (D2 = 43.95), and clusters III and VI (D2 = 42.29). Cluster IV had the least inter-cluster distance (D2 = 22.82) with cluster VI. Clusters III, IV, V, and VI had only one genotype each, and hence, the intra-cluster distance was zero. The genotype of cluster V was unique as it had the highest values for fresh and dry root weight per plant, diameter and length of the main root, number of secondary roots per plant, number of berries per plant, and girth of stem with a high seed yield per plant. Cluster VI was desirable in respect of days to 50 per cent flowering and days to root harvest. Cluster II exhibited the highest value for height of plant and branches per plant. Cluster IV had the highest mean values for the number of seeds per berry. Thus, hybridization among these genotypes can generate desirable transgressive segregants. Aim of Studies: To assess the genetic divergen of Withania somnifera. Place and Duration of Study: Department of Horticulture, College of Agriculture, UAS, GKVK, Bengaluru from 2020-21 to 2021-22. Methodology: The genetic divergence between the genotypes in the population was estimated using formula D2= p (Yit - Yjt) and genetic divergence among 32 Ashwagandha (Withania somnifera (L.) Dunal) accessions of different geographic origins was assessed using Mahalanobis D2 statistics. Observations revealed significant genotypic differences, and accordingly. Results: Observations revealed significant genotypic differences, and accordingly, genotypes were classified into six clusters. Cluster I was the largest, with twenty genotypes, followed by the cluster II, which has eight, and clusters III, IV, V, and VI contained only one most divergent genotype. The maximum divergence was observed between clusters II and III (D2 = 47.90), followed by clusters V and VI (D2 = 43.95), and clusters III and VI (D2 = 42.29). Cluster IV had the least inter-cluster distance (D2 = 22.82) with cluster VI. Clusters III, IV, V, and VI had only one genotype each, and hence, the intra-cluster distance was zero. The genotype of cluster V was unique as it had the highest values for fresh and dry root weight per plant, diameter and length of the main root, number of secondary roots per plant, number of berries per plant, and girth of stem with a high seed yield per plant. Cluster VI was desirable in respect of days to 50 per cent flowering and days to root harvest. Cluster II exhibited the highest value for height of plant and branches per plant. Cluster IV had the highest mean values for the number of seeds per berry Conclusion of finding: Significant genetic divergence and complementarity for most features has been shown by the genotypes of clusters II and III and clusters V and VI. This could lead to significant genetic improvement for particular characters and their selection for new varieties.

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Withania somnifera- A Boom for Cancer Patients

Abstract: This is a special issue as an outcome of 'RAPSCON-2019' sponsored by APTI and organized by Sri

Cited by 3 publications

References 0 publications

Anti-proliferative effects of Rhamnus frangula miller leaf and bark extracts on HEK293 and MCF-7 cell lines and evaluation of Bax and Bcl-2 genes expression level in MCF-7 cells

Anti-proliferative effects of Rhamnus frangula miller leaf and bark extracts on HEK293 and MCF-7 cell lines and evaluation of Bax and Bcl-2 genes expression level in MCF-7 cells

Assessment of Antioxidant Activity of Withania somnifera in Equine

Genetic Divergence of Ashwagandha (Withania somnifera (L.) Dunal) for Growth and Yield Traits

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