With regard to glycohemoglobin measurement: are we sure that high-performance liquid chromatography currently works in the clinical routine?

Matteucci, Elena; Milioni, C; Biasci, E; Bertoni, C; Boldrini, E; Giampietro, Ottavio

doi:10.1007/s005920050099

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…The performance of these devices in this study was consistent with that found by other researchers (DCA 2000 [27][28][29][30][31][32][33][34][35][36][37] ; DCA Vantage [38][39][40]. During this study, the analytical quality of the established POCT devices and a number of new devices was assessed along with stakeholder satisfaction relating to the POCT service.…”

Section: Discussionsupporting

confidence: 87%

Optimizing Point-of-Care Testing for Diabetes Management in a Rural Australian General Practice

Motta¹,

Shephard²,

Halls³

et al. 2015

Point of Care: The Journal of Near-Patient Testing &Amp; Technology

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Since 2004, the Flinders University International Centre for Point-of-Care Testing has assisted the Mallee Track Health & Community Service with a point-of-care testing (POCT) service for diabetes management in rural Australia. The POCT devices originally implemented were the DCA 2000 (hemoglobin A1c) and the Cholestech (glucose, lipids). In recent years, new options for POCT have become available, including the DCA Vantage (hemoglobin A1c), the StatStrip (glucose), and the Piccolo (glucose, lipids). The analytical performance of the POCT devices was evaluated using a patient comparison study (n = 103) to measure accuracy and using on-site quality control testing to measure between-day imprecision. Stakeholder satisfaction was assessed using questionnaires for patients with diabetes (n = 60) and focus interviews with the POCT operator and general practitioner. The DCAVantage and Piccolo exhibited sound analytical performance. A statistically significant improvement in satisfaction with the new devices was reported by the patients (Fisher exact probability, P < 0.05). All stakeholders showed high levels of satisfaction with POCT in terms of convenience, acceptability, and confidence in test results. This study supports the effective use of POCT in rural general practice settings for diabetes management.D iabetes mellitus is a major health problem reaching epidemic proportions in many parts of the world. 1 There were approximately 371 million people living with diabetes worldwide in 2012, constituting 8.3% of the world's adult population. 2 In Australia, there are more than 1.7 million people living with diabetes, with prevalence set to double by 2025. 3,4 Diabetes causes significant morbidity and mortality, primarily from cardiovascular complications, renal and eye disease, and limb amputations. 5 In addition to the impact on the health of individuals with the disease, diabetes causes significant economic impact on individuals, families, health systems, and countries across the world. 6 Pathology testing plays a vital role in managing patients with diabetes and monitoring of complications of the disease. Pathology tests performed as part of a patient's diabetes review include glucose, hemoglobin A1c (HbA1c), urine albumin-creatinine ratio (ACR), and lipids (total cholesterol, high-and low-density lipoprotein [HDL and LDL] cholesterol, and triglyceride). 7,8 These tests can now be performed safely and effectively through pointof-care testing (POCT). Point-of-care testing is pathology testing performed in a clinical setting at the time of patient consultation.convenience, acceptability, and confidence in test results. Overall, the GP and the POCT operator were very satisfied with all aspects of the new POCT service. Limitations of This StudyOnly 1 POCT operator and 1 GP took part in this project, which meant that their satisfaction levels could not be statistically analyzed. Nonetheless, this staffing structure reflected the "realtime" nature of workflow within the MTH&CS clinic.Owing to the nature of this project f...

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Section: Discussionsupporting

confidence: 87%

Optimizing Point-of-Care Testing for Diabetes Management in a Rural Australian General Practice

Motta¹,

Shephard²,

Halls³

et al. 2015

Point of Care: The Journal of Near-Patient Testing &Amp; Technology

View full text Add to dashboard Cite

show abstract

Evaluation of diagnostic reliability of DCA 2000 for rapid and simple monitoring of HbA1c

Arsie¹,

Marchioro²,

Lapolla

et al. 2000

Acta Diabetologica

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The monitoring of diabetic patients by evaluating glycated protein levels is now widely accepted and performed. The microchromatographic version of the high performance liquid chromatography method is the technique most frequently used in clinical practice. The DCA 2000 instrument (Bayer Diagnostics, Milan, Italy), based on an immunochemical technique, has been proposed for the rapid and simple evaluation of HbAlc, using even capillary blood. We evaluated 171 subjects including 22 healthy volunteers, 78 type 2 diabetic patients with different degrees of metabolic control, 11 women affected by gestational diabetes mellitus (GDM), 6 patients with hyperlipemia, 38 patients with chronic renal failure, 13 diabetic patients with chronic renal failure, and 3 patients with hemoglobinopathies. The DCA 2000 model was compared with the Diamat HPLC system. Data from within-run imprecision studies showed excellent precision, for both DCA 2000 and the HPLC system. The correlation between the two different systems, as shown by other statistical evaluations, was good (y = 0.911x + 0.462, r = 0.923). Results from the control group and diabetic patients were used to compare the two methods. Values obtained using the DCA 2000 were significantly lower (p < 0.0001) than those obtained with the HPLC system, in both healthy subjects and diabetic patients. To detect possible interferences, selected samples were analyzed from patients with hyperlipemia, diabetes and chronic renal failure, and hemoglobinopathies. While in the case of hyperlipemia, an acceptable correlation coefficient between the two systems was confirmed (y = 1.047x - 1.236, r = 0.876), in the case of chronic renal failure the correlation turned out to be very low (y = 0.254x + 3.456, r = 0.203). Our results indicate that the DCA 2000 gives accurate and reliable results in the clinical field of interest.

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Agreement between HbA1c measured by DCA 2000 and by HPLC: Effects of fetal hemoglobin concentrations

Diem¹,

Wälchli²,

Mullis³

et al. 2004

Archives of Medical Research

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With regard to glycohemoglobin measurement: are we sure that high-performance liquid chromatography currently works in the clinical routine?

Cited by 3 publications

References 10 publications

Optimizing Point-of-Care Testing for Diabetes Management in a Rural Australian General Practice

Optimizing Point-of-Care Testing for Diabetes Management in a Rural Australian General Practice

Evaluation of diagnostic reliability of DCA 2000 for rapid and simple monitoring of HbA1c

Agreement between HbA1c measured by DCA 2000 and by HPLC: Effects of fetal hemoglobin concentrations

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