With or Without You: Altered Plant Response to Boron-Deficiency in Hydroponically Grown Grapevines Infected by Grapevine Pinot Gris Virus Suggests a Relation Between Grapevine Leaf Mottling and Deformation Symptom Occurrence and Boron Plant Availability

Buoso, Sara; Pagliari, Laura; Musetti, Rita; Fornasier, Flavio; Martini, Marta; Loschi, Alberto; Fontanella, Maria Chiara; Ermacora, P.

doi:10.3389/fpls.2020.00226

Cited by 20 publications

(31 citation statements)

References 107 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Outcome of new viral entities are often detected in vineyards with several unpredictable features, such as limited understanding of the interaction with their host, their etiology, poor knowledge of route(s) of infection and dispersal, and lack of biological and molecular tools to study the virus ecology. In spite of all these obstacles, it is important to deepen the knowledges of new reported viruses, since, as already demonstrated in many cases, they can play important roles in defining the interaction among the plant, the associated microbes and the environment [10,11,24,31]. In specific, here we have identified two new viruses and now we are going to discuss their potential impact on plant physiology that can go further than a simple pathogen-host relationship.…”

Section: Discussionmentioning

confidence: 75%

Two New Putative Plant Viruses from Wood Metagenomics Analysis of an Esca Diseased Vineyard

Bertazzon

Chitarra

Angelini

et al. 2020

Plants

View full text Add to dashboard Cite

The concept of plant as a holobiont is now spreading among the scientific community and the importance to study plant-associated microorganisms is becoming more and more necessary. Along with bacteria and fungi, also viruses can play important roles during the holobiont-environment interactions. In grapevine, viruses are studied mainly as pathological agents, and many species (more than 80) are known to be able to replicate inside its tissues. In this study two new viral species associated with grape wood tissues are presented, one belongs to the Potyviridae family and one to the Bunyavirales order. Due to the ability of potyviruses to enhance heterologous virus replication, it will be important to assess the presence of such a virus in the grapevine population to understand its ecological role. Furthermore, the association of the cogu-like virus with esca symptomatic samples opens new questions and the necessity of a more detailed characterization of this virus.

show abstract

Section: Discussionmentioning

confidence: 75%

Two New Putative Plant Viruses from Wood Metagenomics Analysis of an Esca Diseased Vineyard

Bertazzon

Chitarra

Angelini

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

“…The affection of boron supply on viral symptom occurrence is well known and it has also been described in other pathosystems [18,19]. In controlled conditions, the GLMD symptoms in GPGV positive grapevines only occurred at boron deficiency conditions, while, at sufficient boron availability, the symptoms were not visible, regardless of GPGV infection [20]. In the same study, the gene expression analyses of two boron exporters BOR1 and BOR2 and two nodulin-26-like intrinsic proteins, NIP5;1 and NIP6;1, revealed that the GPGV presence during boron deficiency conditions significantly alters the expression of these genes.…”

Section: Introductionmentioning

confidence: 82%

“…Boron exporter BOR1 plays a key role in xylem loading and boron distribution within shoots [21], and it is mainly expressed in the root, but also in other tissues [22]. At boron deficiency, the expression of this gene was elevated in grapevine leaves and roots, while, at the GPGV infection during the boron deficiency, its expression was significantly higher than in healthy plants [20]. BOR2 is an efflux-type boron transporter that has been proposed to facilitate the effective cross linking of RG-II by boron in the cell wall [22].…”

Section: Introductionmentioning

confidence: 99%

“…This protein is strongly expressed in the root tissues, and it is essential for root cell elongation under low boron conditions [23]. Different expressions of this gene were observed in grapevine roots and leaves under boron deficiency, where the expression was downregulated in healthy plant roots and upregulated in leaves, while, in GPGV infected plants, the expression was significantly downregulated in both roots and leaves when compared to healthy plants [20].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Incidence of GLMD-Like Symptoms on Grapevines Naturally Infected by Grapevine Pinot gris virus, Boron Content and Gene Expression Analysis of Boron Metabolism Genes

et al. 2021

View full text Add to dashboard Cite

Grapevine Pinot gris virus (GPGV) is considered to be a causal agent of Grapevine Leaf Mottling and Deformation (GLMD) disease that has been reported worldwide through the grapevine-growing regions. Seven grapevines that were collected from a vineyard in the Czech Republic were tested for the presence of GPGV in leaf and phloem tissues. Each of the seven grapevines was infected by GPGV, from which sic symptoms were mostly shown without a typical mottling. The phylogeny based on RNA-dependent RNA polymerase and movement/coat protein sequences indicated the same origin of the GPGV isolates. The GPGV titer was the highest in the grapevines with the highest GLMD-like symptoms; however, some of the grapevines with milder GLMD-like symptoms had a lower GPGV titer than the asymptomatic grapevine. Soil analysis showed uneven boron content in the direct vicinity of the grapevines, while the boron content in the grapevines was more, even showing no boron deficiency. The quantitative analysis of selected gene expressions associated with boron efflux and transport only partially explained the boron content in the soil and grapevines and only in the grapevines growing in soils with the highest or lowest boron contents. The VvBor2 and VvNIP5 genes had a higher expression and VvNIP6 had a lower expression in the grapevine growing in the soil with the lowest boron content, while a low expression of VvBor1 and VvBor2 was observed in the grapevine that was grown in the soil with the highest boron content.

show abstract

“…The geographic uniqueness of GPGV isolates was confirmed by Eichmeier et al [ 3 , 5 , 10 , 24 ]. Given the similarities between GLMD and boron deficiency symptoms in grapevines, Buoso et al [ 41 ] suggested that GPGV interferes in boron homeostasis. However, two studies suggested that the association between the symptoms and the presence of the virus is unclear [ 2 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Survey and Diversity of Grapevine Pinot gris virus in Algeria and Comprehensive High-Throughput Small RNA Sequencing Analysis of Two Isolates from Vitis vinifera cv. Sabel Revealing High Viral Diversity

Eichmeier

Peňázová

Čechová

et al. 2020

Genes

View full text Add to dashboard Cite

Grapevine Pinot gris virus (GPGV) is a putative causal agent of grapevine leaf mottling and deformation disease that has been reported worldwide throughout the grapevine-growing regions. Fifty-four grapevines collected from five Algerian grapevine-growing regions were tested for the presence of GPGV in phloem tissues. Eight of the tested grapevines were infected by GPGV. Viromes of two selected Vitis vinifera cv. Sabel grapevines infected by GPGV and showing virus-like symptoms were analyzed by small RNA sequencing. Phylogenetic analyses of the partial coding sequence (cds) of the RNA-dependent RNA polymerase (RdRp) domain showed that all Algerian GPGV isolates were grouped with some already-described asymptomatic isolates. This study provides the first survey of the occurrence of GPGV in Algeria. Moreover, Grapevine fleck virus, Grapevine rupestris stem pitting-associated virus, Grapevine virus B, Grapevine rupestris vein feathering virus, Hop stunt viroid and Grapevine yellow speckle viroid 1 were detected in Algeria for the first time.

show abstract

With or Without You: Altered Plant Response to Boron-Deficiency in Hydroponically Grown Grapevines Infected by Grapevine Pinot Gris Virus Suggests a Relation Between Grapevine Leaf Mottling and Deformation Symptom Occurrence and Boron Plant Availability

Cited by 20 publications

References 107 publications

Two New Putative Plant Viruses from Wood Metagenomics Analysis of an Esca Diseased Vineyard

Two New Putative Plant Viruses from Wood Metagenomics Analysis of an Esca Diseased Vineyard

Incidence of GLMD-Like Symptoms on Grapevines Naturally Infected by Grapevine Pinot gris virus, Boron Content and Gene Expression Analysis of Boron Metabolism Genes

Survey and Diversity of Grapevine Pinot gris virus in Algeria and Comprehensive High-Throughput Small RNA Sequencing Analysis of Two Isolates from Vitis vinifera cv. Sabel Revealing High Viral Diversity

Contact Info

Product

Resources

About