WISP-2: A Serum-Inducible Gene Differentially Expressed in Human Normal Breast Epithelial Cells and in MCF-7 Breast Tumor Cells

Zoubine, Mikhail N.; Banerjee, Snigdha; Saxena, Neela K.; Campbell, Donald R.

doi:10.1006/bbrc.2001.4584

Cited by 42 publications

(35 citation statements)

References 17 publications

(15 reference statements)

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“…Semiconfluent (50-60%) MCF-7 cells were transiently transfected with vector alone or WISP-2/CCN5 siRNA (WISP-2/CCN5#1) containing vectors in serum-free Opti-MEM I as indicated in Materials and Methods. WISP-2/CCN5 protein level was examined by immuno-Western blot analysis with WISP-2-specific polyclonal antibody generated in our laboratory (8) or h-actin monoclonal antibody (top ). The ability of WISP-2/CCN5 siRNA to inhibit EGFinduced proliferation at 48 hours after transfection was determined in MCF-7 cells using bromodeoxyuridine (BrdU ) ELISA (bottom ).…”

Section: Resultsmentioning

confidence: 99%

“…The digoxigenin high prime DNA labeling and detection kit was obtained from Roche Diagnostics GmbH (Indianapolis, MD). WISP-2/ CCN5 rabbit polyclonal antibody was generated previously in our laboratory (8).…”

Section: Methodsmentioning

confidence: 99%

“…Total RNAs from different samples were reverse transcribed into cDNAs and PCR amplified with pS2, EGFR, and GAPDH specific primers following the method described earlier (8). The sequences of primers are pS2, 5V-ATGGCCACCATGGAGAA-CAA-3V(forward) and 5V-ATAGAAGCACCAGGGGACCC-3V (reverse); EGFR, 5V-GAGTCGGGCTCTGGAGGAAA-3V(forward) and 5V-GCTCCTTCAGTCCGGTTTTA-3V (reverse); and GAPDH, 5V-ATGAGAAGTATGACAACAGCC-3V (forward) and 5V-TGAGTCCTTCCACGATACC-3V (reverse).…”

Section: Reverse Transcription-pcr Analysismentioning

confidence: 99%

“…Recent studies from our laboratory and others have suggested that WISP-2/CCN5 has a particular relevance to human breast disease (1,7,8). This is because WISP-2/CCN5 mRNA and protein levels were elevated in different human breast tumor-derived cell lines as well as in breast tumor samples while being barely detectable, or undetectable, in normal breast epithelial cells (7)(8)(9). Additionally, abnormal expression of the WISP-2/CCN5 gene was significantly higher in noninvasive carcinoma cells compared with invasive carcinoma cells.…”

Section: Introductionmentioning

confidence: 99%

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Epidermal Growth Factor Induces WISP-2/CCN5 Expression in Estrogen Receptor-α-Positive Breast Tumor Cells through Multiple Molecular Cross-talks

Banerjee

Sengupta²,

Saxena³

et al. 2005

Molecular Cancer Research

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Epidermal growth factor (EGF) is a mitogen for estrogen receptor (ER) -positive breast tumor cells, and it has been proven that EGF occasionally mimicked estrogen action and cross-talks with ER-A to exert its activity. Therefore, the present study was undertaken to explore whether EGF is able to modulate the expression of Wnt-1-induced signaling protein-2/connective tissue growth factor/cysteine-rich 61/nephroblastoma overexpressed 5 (WISP-2/CCN5), an estrogenresponsive gene, in normal and transformed cell lines of the human breast and, if so, whether this induction is critical for EGF mitogenesis and what downstream signaling pathways are associated with this event. Here, we show that EGF-induced WISP-2 expression in ER-and EGF receptor -positive noninvasive MCF-7 breast tumor cells was dose and time dependent and that expression was modulated at transcription level. A synergism was seen in combination with estrogen. Moreover, small interfering RNA -mediated inhibition of WISP-2/CCN5 activity in MCF-7 cells resulted in abrogation of proliferation by EGF. The multiple molecular cross-talks, including the interactions between phosphatidylinositol 3-kinase/Akt and mitogen-activated protein kinase signaling pathways and two diverse receptors (i.e., ER-A and EGFR), were essential in the event of EGF-induced WISP-2/CCN5 up-regulation in MCF-7 cells. Moreover, EGF action on WISP-2/CCN5 is restricted to ER-and EGFR-positive noninvasive breast tumor cells, and this

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Reverse Transcription-pcr Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Epidermal Growth Factor Induces WISP-2/CCN5 Expression in Estrogen Receptor-α-Positive Breast Tumor Cells through Multiple Molecular Cross-talks

Banerjee

Sengupta²,

Saxena³

et al. 2005

Molecular Cancer Research

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“…24 In situ hybridization was carried out essentially as described by the manufacturer (Innogenex, San Ramon, CA) with minor modifications. Five-micrometer paraffin sections were deparaffinized with xylene, hydrated in ethanol/ water solutions and digested with proteinase K (ready to use vial) for 10 min followed by post-fixation in 1% formaldehyde/1ϫ PBS for 10 min at room temperature.…”

Section: Probe Preparation and In Situ Hybridizationmentioning

confidence: 99%

Neuropilin‐1 is differentially expressed in myoepithelial cells and vascular smooth muscle cells in preneoplastic and neoplastic human breast: A possible marker for the progression of breast cancer

Stephenson

Banerjee

Saxena

et al. 2002

Intl Journal of Cancer

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109

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The expression and distribution of neuropilin-1 (NRP-1) was examined in the samples of normal human breast tissues and in non-neoplastic and neoplastic areas of breast tissue removed for carcinoma using RT-PCR as well as conventional and tissue microarrays immunohistochemical analyses. The NRP-1 mRNA expression was significantly higher in neoplastic tissues as compared to normal breast samples. Immunohistochemically, the myoepithelial cells of the mammary ducts and lobules display positive reactions for NRP-1, whereas the inner ductal and lobular epithelial cell layers failed to react. The myoepithelial cells of ducts and lobules in both neoplastic and non-neoplastic tissue specimens displayed a stronger positive reaction for NRP-1 than those in the normal breast. A positive reaction for NRP-1, but with a gradual reduction in intensity, was observed in the myoepithelial cells of ducts with atypical epithelial hyperplasia and ductal carcinoma in situ (DCIS). The reaction was undetected or minimally detected in the areas of invasive carcinoma. NRP-1 positive immunolabeling was also localized in the vascular smooth muscle cells and in some endothelial cells of the blood vessels in normal, non-neoplastic and neoplastic breast tissue samples. In areas of breast carcinoma, NRP-1 immunolabeling was more prominent in both vascular smooth muscle cells and in some endothelial cells than in similar cells in normal breast. The specificity of the newly developed antibody for NRP-1 was confirmed by in situ hybridization with DIG-labeled PCR generated probe. These results suggest that NRP-1 may be a multiple function protein in human breast and may be involved in the induction of local invasiveness of neoplasia and angiogenesis and have direct relevance to the progression of breast cancer.

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A Monoclonal Antibody Approach to CCN5 Domain Analysis

Lan

McKeon

Russo

et al. 2010

CCN Proteins in Health and Disease

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WISP-2: A Serum-Inducible Gene Differentially Expressed in Human Normal Breast Epithelial Cells and in MCF-7 Breast Tumor Cells

Cited by 42 publications

References 17 publications

Epidermal Growth Factor Induces WISP-2/CCN5 Expression in Estrogen Receptor-α-Positive Breast Tumor Cells through Multiple Molecular Cross-talks

Epidermal Growth Factor Induces WISP-2/CCN5 Expression in Estrogen Receptor-α-Positive Breast Tumor Cells through Multiple Molecular Cross-talks

Neuropilin‐1 is differentially expressed in myoepithelial cells and vascular smooth muscle cells in preneoplastic and neoplastic human breast: A possible marker for the progression of breast cancer

A Monoclonal Antibody Approach to CCN5 Domain Analysis

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