Wirkungsbezogene Umsetzungen auf der DC-Platte (direktes Biomonitoring)

Prevention of False-Positive Results: Development of an HPTLC Autographic Assay for the Detection of Natural Tyrosinase Inhibitors

“…For extracts containing very polar substances, which were retained at the application zone, a third mobile phase with a higher elution strength [ethyl acetate, methanol, water, formic acid (50 : 10 : 7 : 1, v/v/v/v; MPIII)] was employed. As the bioassay is performed directly on the HPTLC plate, it was necessary to remove all solvents of the mobile phase, especially formic acid and toluene because even small residues can interfere with the assay [11]. After development and normal drying of the HPTLC plate for 5 min, the residue of the formic acid was first neutralised by placing the plate for 10 min in the rear trough of a twin trough chamber containing ammonia solution in the front trough.…”