Wirkung von einigen Pflanzenwachstumsregulatoren auf verschiedene Prokaryonten und deren Viren

J of Basic Microbiology

1978

In the agar diffusion test 24 triazines were investigated with regard to their action on the multiplication of DNA phages (λ and LPP‐1) and RNA phages (M 12 and Qβ). In several cases the amount of plaques was diminished or increased depending on the kind of triazine and virus. The investigations demonstrate the triazines to be able to interfere with the formation of plaques by virulent and temperate viruses of procaryotes.

Wirkung von 1,3,5‐Triazinen auf verschiedene Prokaryontenviren und ihre Wirte

Stenz

J of Basic Microbiology

1978

Wirkung des Detergens Metaupon auf die Vermehrung verschiedener Phagen

J of Basic Microbiology

Stenz

1979

Self Cite

As several other surfactants do, the detergent Metaupon acts on the multiplication of bacteriophages. We investigated the influence of Metaupon on the phages 0 105 and 1, the cyanophage LPP-1, and the RNA-phages f 2 , M 12, and QB by means of the agar diffusion test, pour plate test, adsorption test, and one-step growth test. The action of Metaupon on the free phages was also tested. Metaupon inhibits the formation of plaques by the phages with exception of 3,. With the phages f 2 and M 12 the substance increases the amount of plaques depending on concentration. The main mode of action of Metaupon was found to be the inhibition of the adsorption of the phages to the host cells. Only in the case of CD 105 free phages were inactivated.Es ist bekannt, daB Detergentien auf Bakteriophagen wirken konnen. Als Wirkorte solcher Verbindungen wurden die freien Phagen, die Adsorption der Phagen an die

Geförderte Plaquebildung in RNA‐Phagen/Bakterien‐Systemen unter dem Einfluß oberflächenaktiver Präparate

1985

J. Basic Microbiol.

The effect of 21 surfactants on the plaque formation was tested in three RNA-phages/Escherichia coli systems and in one RNA-phage/Pseudomonas aeruginosa system. Especially anionic detergents proved to be able to influence the plaque formation substantially. In high concentrations of Metaupon, Fekunil 602, Fekunil S-BA, Emulgator W 270, and Emulgator O-BA the plaque formation by the phages M 12 and f2 was inhibited and in low concentrations it was promoted. In the system Q beta/E. coli AB 301 the effect of the detergents mentioned was restricted to the prevention of the appearance of plaques. The detergent E 30 brought about only plaque inhibitions, too, in all used RNA-phages/E. coli systems. The treatment with effective detergents in the system PP7/P. aeruginosa, however, increased only the number of plaques. This phenomenon was evident in the formation of radiate plaque patterns in the lawn of bacteria. In the case of RNA-phages of E. coli the formation of such trains of lysis depends on the choice of the nutritive medium. The addition of the detergent Fekunil 602 at different times after the contact between phages and host bacteria affects the length of the beams of lysis. The ionogenity of surfactants seems to be of importance for the formation of radiate plaque patterns, since the tested cationic compound and the nonionic surfactants, contrary to anionic surfactants, did not cause any beams of lysis.