Wireless, Remote-Query, and High Sensitivity Escherichia coli O157:H7 Biosensor Based on the Recognition Action of Concanavalin A

Abstract

Escherichia coli O157: H7 is detected using a remote-query (wireless, passive) magnetoelastic sensor platform to which a 1 µm-thick layer of Bayhydrol 110 and then a layer of functionalized mannose is applied. The multivalent binding of lectin concanavalin A (Con A) to the E. coli surface O-antigen and mannose favors the strong adhesion of E. coli to the mannose modified magnetoelastic sensor; E. coli is rigidly and strongly attached on the mannose-modified sensor through Con A, which works as a bridge to bind to the E. coli surface O-antigen and mannose modified sensor surface. As E. coli is bound to the sensor its resonance frequency shifts, enabling quantification of E. coli concentration with a limit of detection of 60 cells/mL and a linear logarithmic response range of 6.0×101 to 6.1×109 cells/mL. The analysis can be directly conducted without incubation, and completed in 3 h or less.

A ternary hybrid CdS/Pt–TiO2 nanotube structure for photoelectrocatalytic bactericidal effects on Escherichia Coli

“…Rapid, accurate methods for bacterial detection and effective bactericidal materials are essential with respect to food poisoning as well as water contamination, and therefore, treatment options. Quantitative, sensitive, and specific detection methods developed over the past several decades include DNA and RNA probes [2,3], immunomagnetic separation (ISM) analysis [4,5], and wireless magnetoelastic biosensors [6,7]. As one of the most effective bactericidal materials, TiO 2 photocatalyst has demonstrated the successful inactivation of bacteria, viruses, and cancer cell [8][9][10][11][12].…”

Dual channel detection of ultra low concentration of bacteria in real time by scanning fluorescence correlation spectroscopy

“…In the last years different technique have been developed such as DNA microarray (Ahn and Walt, 2005; Kim et al , 2005; Cho and Tiedje, 2001), PT-PCR (Malorny et al , 2008; Kramer et al , 2009; Mafu et al , 2009), enzyme-linked immunosorbent assay (ELISA) (Ferguson et al , 2000; Tamminen et al , 2004), surface plasmon resonance (SPR) (Maalouf et al , 2007; Oh et al , 2005; Dudak and Boyaci, 2009), magnetic nanoparticle based immunoassay (Malorny et al , 2008; Gu et al , 2003), various formats of biosensors (Gehring et al , 1998; Liao and Ho, 2009; Lu et al , 2009; Wong et al , 2002) and flow cytometry (Busam et al , 2007; Hahn et al , 2008; Hibi et al , 2007; Qin et al , 2008; Moragues et al , 2004; Ferrari et al , 2004). …”

Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer

“…Moreover, aptamer-based interface has also been used for enrichment and detection of multiple cancer cells [14]. Despite the extensive effort launched, a growing interest has been currently focused on the use of lectin-carbohydrate recognition specificities as an alternative way for designing biominic interface to anchor cells because lectin-carbohydrate interactions play a crucial role in many cellular processes including cell adhesion, trafficking, metastasis, and immune response [15][16][17]. Therefore, the development of simple, inexpensive, reliable and rapid strategies for fabricating lectin interface is highly desirable.…”