Wipe test for the detection of indoor allergens

Polzius, R.; Wuske, Thomas; Mahn, J.A.

doi:10.1046/j.0105-4538.2001.documendoc.doc.x

Cited by 4 publications

(5 citation statements)

References 9 publications

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“…Several other alternatives to household allergen collection using a vacuum cleaner have been assessed (Polzius et al, 2002;Sercombe et al, 2005). A quasireal-time commercial instrument with visual display of allergen level has been developed (Drager Bio-Check Allergen Control; Polzius et al, 2002) and compared to vacuum-cleaner based sampling with mixed results (Polzius et al, 2002;Sercombe et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…A quasireal-time commercial instrument with visual display of allergen level has been developed (Drager Bio-Check Allergen Control; Polzius et al, 2002) and compared to vacuum-cleaner based sampling with mixed results (Polzius et al, 2002;Sercombe et al, 2005). One shortcoming is that a separate device is necessary for each allergen of interest.…”

Section: Discussionmentioning

confidence: 99%

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Use of an Electrostatic Dust Cloth for Self-Administered Home Allergen Collection

et al. 2008

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Most epidemiologic studies employ a vacuum cleaner used by a trained technician to collect household allergens. This approach is labor intensive, equipment dependent, and impractical if study subjects reside over a wide geographic area. We examined the feasibility of a self-administered dust collection method, using an electrostatic cloth sent by conventional mail, to obtain allergen measurements. Thirty-two nonasthmatic twins from the California Twin Program wiped areas in the family room, kitchen, and bedroom, according to standardized instructions, and returned the cloths by mail. Allergen concentrations for Der-p-1, Der-f-1, Fel-d-1, and Bla-g-2 were determined using ELISA, and intrahouse and room-to-room concentrations were compared. Der-p-1 and Fel-d-1 were found in most homes, with highest concentrations in bedrooms and kitchens, respectively. Der-f-1 and Bla-g-2 were rarely found. Intrahouse Der-p-1 and Fel-d-1 concentrations were highly correlated and statistically significant (for Der-p-1, bedroom vs. kitchen,p= .0003, bedroom vs. family room,p= .0001, and family room vs. kitchen,p= .002; for Fel-d-1, bedroom vs. kitchen,p= .0004, bedroom vs. family room,p< .0001, and family room vs. kitchen,p= .0001). Reported cat ownership was strongly correlated with household Fel-d-1 concentrations (p< .005). In another comparison from different homes of children enrolled in the La Casa atopy prevention study, allergen concentrations measured from dust collected by a single operator from the left and right half of the same room in 21 homes were compared. Levels of Bla-g-2, Der-p-1, and Fel-d-1 concentrations collected from right and left halves of the same room were highly correlated, withr2ranging from .7 to .9, and were highly statistically significant (allpvalues < .01). We conclude that nonintrusive and self-administered dust collection, using commercially available electrostatic dust cloths, sent by conventional mail services, is a promising alternative to technician-collected vacuumed dust for measuring indoor allergens in population-based studies, although further validation of the method is necessary.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Use of an Electrostatic Dust Cloth for Self-Administered Home Allergen Collection

et al. 2008

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“…Mite group 1 [125] and mite group 2 [126] allergen measurements have been considered as a gold standard for monitoring mite allergen extracts as well as dust mite allergen levels. Twosite ELISA have been developed using lateral flow technology, and can be used as rapid tests by patients themselves or by investigators, to make decisions about exposure and about allergen avoidance something like lateral flow test [127,128] …”

Section: (5) Two-site Elisamentioning

confidence: 99%

Recombinant Allergens for Diagnosis and Immunotherapy of Allergic Disorders, with Emphasis on Cockroach Allergy

Jeong¹,

Hong²,

Yong³

2006

Current Protein and Peptide Science

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The prevalence of allergic disorders has increased over the past few decades and the quality of life has been significantly influenced at least for the allergic subjects. Allergen avoidance is thought to be the best way of preventing clinical manifestation of the disease, however, it is not possible for some allergens, and other pharmacological and/or immunological treatment has to be made. Repetitive injection of sensitized allergens to the patients (immunotherapy) is the only known curative approach to the disease even though the exact mechanism is not clear to date. Crude extract of allergens has lots of shortcomings which might arouse unexpected results. Genetic engineering and recombinant allergens are thought to be one of the alternative ways to overcome these limitations. Genetic engineering could facilitate the investigation of immune responses of the subjects especially on B cell and T cell epitopes, and produce the therapeutic allergens which might minimize the possible side effects. Furthermore, conjugation of immuno-modulatory molecules such as CpG-ODN, cytokines, or toxins which could act specifically to the given allergens, and maleylation of the allergens could maximize the prophylactic or therapeutic effect. Immunotherapies for the pollen allergy and insect sting allergy have been thought to be successful. House dust mite allergy and cockroach allergy have been reported less beneficial by immunotherapeutic approaches. Cockroaches are one of the most important causes of asthma, and severe complications are often reported in the children in city dwellers with low-incomes. The studies of the biological functions of cockroach allergens and the use of recombinant allergens should allow understanding of mechanisms of cockroach-elicited allergic disorders and development of allergen-specific and sensitive diagnostics and tailored therapeutic approaches in the future.

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“…Conventionally, immunoassays such as enzymelinked immunosorbent assay (ELISA) or lateral flow immunochromatographic assay are used for one-time measurement. (10)(11)(12)(13)(14) Other state-of-the-art methods for measuring the allergens have also been reported. (17) Despite such remarkable progress in the development of allergen biosensors, the difficulties of repeatedly using the same sensor surface by regenerating it after each measurement have been drawbacks in the development of an immunosensor for monitoring.…”

Section: Introductionmentioning

confidence: 99%

Semicontinuous Measurement of Mite Allergen (Der f 2) Using a Surface Acoustic Wave Immunosensor under Moderate pH for Long Sensor Lifetime

2017

Sensors and Materials

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In this work, we developed a surface-acoustic-wave (SAW)-based immunosensor for the monitoring of house dust mite (HDM) allergen, Dermatophagoides farinae group 2 (Der f 2). The SAW immunosensor was fabricated by modifying the sensor surface with a self-assembled monolayer of ORLA85 protein and immobilized capture antibodies (cAb) for Der f 2. Because of the pH-insensitive property of ORLA85, the sensor surface could be used for successive measurement and regeneration of the sensor surface. In experiments, first, the conditions for cAb immobilization on the sensor surface were optimized, and then the sensor properties were investigated. These revealed the limit of detection of the SAW immunosensor for Der f 2 to be 6.3 ng/mL and hence, the sensor's high selectivity to Der f 2. Finally, the optimum pH for regeneration, at which the regeneration efficiency and sensor output were balanced, was investigated. It showed that the use of pH 4.0 HCl for regeneration allowed reproducible sensor outputs, and less damage to immobilized cAb; thus, longer sensor lifetime could be expected. The results allowed us to anticipate that the SAW immunosensor would be usable for sensitive, selective, and repeated measurement of Der f 2 with a longer lifetime, and it would be a promising sensor for HDM allergen monitoring.

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Wipe test for the detection of indoor allergens

Cited by 4 publications

References 9 publications

Use of an Electrostatic Dust Cloth for Self-Administered Home Allergen Collection

Use of an Electrostatic Dust Cloth for Self-Administered Home Allergen Collection

Recombinant Allergens for Diagnosis and Immunotherapy of Allergic Disorders, with Emphasis on Cockroach Allergy

Semicontinuous Measurement of Mite Allergen (Der f 2) Using a Surface Acoustic Wave Immunosensor under Moderate pH for Long Sensor Lifetime

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