Winter disease outbreak in sea-bream (Sparus aurata) associated with Pseudomonas anguilliseptica infection

Doménech, Ana; Fernández‐Garayzábal, J. F.; Lawson, Paul A.; García, José Ángel López; Cutuli, M. Teresa; Blanco, M. Mar; Gibello, Alicia; Moreno, Miguel A.; Collins, Matthew D.; Domı́nguez, Lucas

doi:10.1016/s0044-8486(97)00069-0

Cited by 65 publications

(51 citation statements)

References 17 publications

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“…Pseudoalteromonas haloplanktis was mainly isolated in one spring sampling associated to a disease outbreak. None of our strains corresponded to Pseudomonas anguilliseptica, isolated in Spain and Portugal in recent years, and associated with the 'winter disease' (Berthe et al 1995, Domenech et al 1997. Gram-positive bacteria recorded as pathogenic for fish, mainly members of the lactic acid bacteria, were absent among the isolates recovered from the fish analysed.…”

Section: Discussionmentioning

confidence: 73%

“…Such bacteria can cause problems when conditions favour their proliferation (Rodgers & Furones 1998), thus making the differentiation between primary pathogens and opportunistic pathogens difficult. Disease outbreaks in cultured gilthead sea bream include Vibrio spp., Photobacterium damselae, Pseudomonas spp., and Flexibacter maritimus (Breuil & Haffner 1990, Vera et al 1991, Balebona et al 1995, 1998b, Berthe et al 1995, Bovo et al 1995, Sedano et al 1996, Domenech et al 1997, Baptista et al 1999). These species have been recovered from moribund or recently dead fish showing clinical signs.…”

Section: Introductionmentioning

confidence: 99%

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Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms

Pujalte¹,

Sitjà‐Bobadilla²,

Álvarez-Pellitero³

et al. 2003

Dis. Aquat. Org.

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A bacteriological survey of gilthead sea bream Sparus aurata from different fish farms and culture systems on the Spanish Mediterranean coast was conducted. Three different studies were performed. Study A included hatchery-reared larvae; Study B, periodic examination of randomly sampled growing fish; and Study C, growing fish sampled only during mortality/morbidity events. In Studies B and C, sea cages, earth ponds and indoor tanks were surveyed, and in both cases diseased (showing clinical signs) and non-diseased fish were included. In Study A, a shift from Vibrio spp. (30 d after hatching) to oxidative species (60 d after hatching) was detected, and no mortality events were registered. The percentage of fish yielding bacterial growth were similar in Studies B and C, reaching 57.4 and 61.3%, respectively. A statistically significant relationship between the bacterial carriage and the type of facility was only found in Study B, showing that fish from sea cages had a higher bacterial occurrence than fish from other facilities. A statistically significant relationship between bacterial carriage and signs of disease was found, although the pattern differed in each study. Thus, in Study B only 36.2% of fish yielding abundant bacterial growth were diseased, versus 68.0% in Study C. In total, 25.0% of the fish examined were diseased. Bacterial species composition was similar in asymptomatic and diseased fish, except for a group of V. ichthyoenteri-like isolates that occurred almost exclusively in asymptomatic fish. Dominant bacterial species were V. harveyi and V. splendidus, followed by V. ichthyoenteri-like isolates, Photobacterium damselae ssp. damselae and V. fisheri. Non-fermenters were less frequent but, among them, unidentified halophilic Cytophaga-Flavobacterium isolates and Pseudoalteromonas haloplanktis were the most abundant. An association of individual species with disease was not clear, which suggests the involvement of mixed infections. KEY WORDS: Sparus aurata · Vibrio harveyi · Vibrio splendidus · Photobacterium damsela · Pseudoalteromonas haloplanktis · Vibrio ichthyoenteri · Mediterranean aquaculture Resale or republication not permitted without written consent of the publisherDis Aquat Org 54: [119][120][121][122][123][124][125][126] 2003 Baptista et al. 1999). These species have been recovered from moribund or recently dead fish showing clinical signs. However, studies on the bacterial flora present in asymptomatic fish are scarce.Several studies have been conducted along the Spanish Mediterranean coast to identify the main bacterial groups present in water and bivalves (Ortigosa et al. 1994a,b, Arias et al. 1999. Some of these bacteria exhibit clear seasonal variation, as Vibrio harveyi dominated with temperatures above 20°C and V. splendidus dominated at temperatures below 20°C (Arias et al. 1999). These and other bacterial species showing seasonal occurrence have been reported as opportunistic pathogens for sea bass and sea bream (Balebona 1998b).In the present study, bacterial car...

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Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms

Pujalte¹,

Sitjà‐Bobadilla²,

Álvarez-Pellitero³

et al. 2003

Dis. Aquat. Org.

View full text Add to dashboard Cite

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“…From 1990, outbreaks of winter disease, an hemor rhagic septicemia also caused by P. anguilliseptica, have been reported in farmed gilthead seabream (Sparus aurata) in the Mediterranean area (Berthe et al, 1995;Domenech et al, 1997;Lopez-Romalde et al, 2003;Romalde et al, 2003). More recently, this patho gen has also been isolated from turbot (Scophthalmus maximus), another valuable marine-fish species cultured in Europe (Lopez- Romalde et al, 2003).…”

mentioning

confidence: 99%

“…anguilliseptica has been described as a fastidious microorganism showing slow growth and weak reactivity on the bacteriological media usually employed for diag nostic purposes in the fish disease laboratories (Domenech et al, 1997;Daly, 1999;). In addition, there are some discrepancies among the phenotypical descriptions reported by differ ent authors (Wakabayashi and Egusa, 1972;Wiklund and Bylund, 1990;Michel et al, 1992;Berthe et al, 1995;Domenech et al, 1997;Daly, 1999). On the other hand, in most cases affected fish do not show typi cal clinical signs which can be associated with P. anguilliseptica.…”

mentioning

confidence: 99%

Development and Validation of a PCR-based Protocol for the Detection of Pseudomonas anguilliseptica

et al. 2004

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ABSTRACT--A PCR-based protocol for the detection of the emerging fish pathogen Pseudomonas anguilliseptica was developed, using the primer set Psan-F/Psan-R designed on the basis of the 16S rRNA sequence.This PCR protocol was optimized and validated by testing 50 target and 38 non-target pure cultures. The method showed a 100% specificity and the detection limit obtained was of 0.7 pgDNA/PCR tube, which equates to 20 or lower bacterial cells. A similar level of sensi tivity was observed when the PCR protocol was applied to fish tissues seeded with bacteria. In addition, the developed PCR assay was also capable of direct detection of P. anguilliseptica from tissues obtained from experimentally infected fish and fish obtained from natural outbreaks in gilthead seabream (Sparus aurata) and turbot (Scophthalmus maximus) farms. This PCR protocol can be a useful tool for a rapid, specific and sensitive diagnosis of fish pseudomonadiasis caused by P. anguilliseptica.

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“…However, in previous studies, some bacterial agents such as Aeromonas hydrophila, , and Pseudomonas anguilliseptica [11][12][13] , have been isolated and some parasites such as Sanguinicolid trematodes 14 have been reported during the outbreaks of WD. In the present study, according to bacteriological examinations, bacterial agents such as Vibrio spp.…”

Section: Bi̇ri̇nci̇oğlu Aydoğan Avcimentioning

confidence: 99%

Pathological Investigations of Winter-like Disease in Cultured Sea Breams (Sparus aurata)

Birincioğlu¹,

Aydoğan²,

Avcı³

2013

Kafkas Univ Vet Fak Derg

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SummaryDuring the January 2008, an outbreak occurred in 15 sea bream farms located in Güllük gulf of Bodrum, Turkey and approximately two million sea breams (Sparus aurata) were died in three weeks. When the outbreak was took place, surface temperature and dissolved oxygen of sea water was measured as 10ºC and 7.9 mg/l on January 10, 2008. For pathological examinations, a total of 65 fish (8-9-month-old) were collected from 9 of 15 farms. At necropsy, extreme abdominal distention, milk-like discharge around the anus and depigmentation of skin were observed in all fish. The liver was swollen and yellowish-white in color. Microscopically, degeneration, severe fatty changes and multifocal necrotic areas were observed in the liver. The similar necrotic areas were also noted in the kidney and spleen. Severe verminous enteritis due to Myxidium spp. was seen in two fish farms. The gills were generally edematous and hyperemic. According to result of bacteriological examinations, Vibrio spp. (farm no: 1) and Staphylococcus spp. (farms no: 1, 8) were isolated and identified in some farms. In conclusion, it is thought that the outbreak occurred in sea breams might be winter disease associated with sudden decreases in water temperature together with induced fatty liver. Keywords: Winter disease, Sea bream, Histopathology, Liver Kültür Çipuralarında (Sparus aurata) Winter-benzeri Hastalık Üzerinde Patolojik İncelemeler ÖzetBodrum Güllük körfezinde bulunan 15 farklı balık çiftliğinde, 10 Ocak 2008 döneminde, kültür çipuralarında (Sparus aurata) görülen bir salgın sonucu üç hafta içinde yaklaşık 2 milyon balık ölümü meydana geldi. Ölümlerin meydana geldiği dönemde, deniz suyunun yüzey sıcaklığı 10ºC ve çözünmüş oksijen miktarı 7.9 mg/l olarak ölçüldü. Çalışmada, salgının görüldüğü 15 işletmenin dokuzundan alınan toplam 65 adet çipura (8-9 aylık) incelendi. Dış bakıda, tüm balıklarda karında aşırı şişkinlik, anus etrafında süt benzeri akıntı ve deride depigmentasyon görüldü. Karaciğerler şişkin ve sarımsı-beyaz renkteydi. Mikroskobik incelemede, karaciğerde dejenerasyon, şiddetli yağlanma ve multifokal nekroz alanları dikkati çekti. Benzer nekroz alanlarına böbrek ve dalaklarda da rastlandı. İki işletmede barsaklarda Myxidium spp.ye bağlı şiddetli paraziter enteritis saptandı. Solungaçların sekunder lamellerinde ödem ve hiperemi oldukça belirgindi. Bakteriyolojik inceleme sonucunda, bir işletmede Vibrio spp. (işletme no: 1), iki işletmede ise Staphylococcus spp. (işletme no: 1, 8) izole ve identifiye edildi. Sonuç olarak, çipuralarda görülen bu ölümlerin, deniz suyu sıcaklığında meydana gelen ani düşüşün neden olduğu karaciğer yağlanmasıyla karakterize Winter hastalığına bağlı olarak oluşabileceği düşünüldü.

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Winter disease outbreak in sea-bream (Sparus aurata) associated with Pseudomonas anguilliseptica infection

Cited by 65 publications

References 17 publications

Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms

Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms

Development and Validation of a PCR-based Protocol for the Detection of Pseudomonas anguilliseptica

Pathological Investigations of Winter-like Disease in Cultured Sea Breams (Sparus aurata)

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