Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae

Nedashkovskaya, Olga I.; Kim, Seung Bum; Han, Suk Kyun; Snauwaert, Cindy; Vancanneyt, Marc; Swings, Jean; Kim, Kyung-Ok; Lysenko, Anatoly M.; Rohde, Manfred; Frolova, Galina M.; Mikhailov, Valery V.; Bae, Kyung Sook

doi:10.1099/ijs.0.63307-0

Cited by 99 publications

(114 citation statements)

References 21 publications

(14 reference statements)

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“…DNA G+C content was determined by the thermal denaturation method (Marmur & Doty, 1962). The DNA G+C content of strain SCB36 T was 36.3 mol%, which is similar to or slightly higher than the values reported for the other Winogradskyella species (32.8-36.1 mol%; Nedashkovskaya et al, 2005;Lau et al, 2005). DNA-DNA hybridization experiments were performed spectrophotometrically using the initial renaturation rate method (De Ley et al, 1970).…”

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confidence: 78%

“…Susceptibility to antibiotics was examined by the routine disc-diffusion plate method after 7 days of incubation on MA at 28 u C. Discs were impregnated with the following antibiotics (mg unless otherwise stated): ampicillin (10), benzylpenicillin (10 U), carbenicillin (100), chloramphenicol (30), doxycycline (10), erythromycin (15), gentamicin (10), kanamycin (30), lincomycin (15), neomycin (30), oleandomycin (15), polymyxin B (300 U), streptomycin (30) and tetracycline (30). Phenotypic characteristics of strain SCB36 T were determined in the same laboratory under the same conditions and using the same methodology as described previously for the type strains of three other Winogradskyella species (Nedashkovskaya et al, 2005). Cells of strain SCB36 T were Gram-reaction-negative single rods, approximately 0.3-0.4 mm in diameter and 0.8-1.8 mm in length ( Supplementary Fig.…”

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confidence: 99%

“…For analysis of the fatty acid composition, strain SCB36 T was grown on MA at 28 u C. When dense growth had developed (24 h), cells were harvested and the fatty acid composition was determined according to the standard protocol of the Microbial Identification System. Fatty acid analysis of strain SCB36 T was carried out in the same laboratory and under the same conditions and methodology as described previously for three other Winogradskyella species (Nedashkovskaya et al, 2005). The cellular fatty acid profile of strain SCB36 T differed markedly from those of the other Winogradskyella species in that the four dominant fatty acids, anteiso-C 15 : 0 , iso-C 15 : 0 , iso-C 15 : 0 3-OH and iso-C 15 : 1 , together accounted for 62 % of the total fatty acids (each accounting for 14-18 % of total), while iso-C 16 : 0 3-OH was only a minor component (Table 2).…”

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confidence: 99%

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Winogradskyella rapida sp. nov., isolated from protein-enriched seawater

Pinhassi¹,

Nedashkovskaya

Hagström³

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Flavobacteria are emerging as an important group of organisms associated with the degradation of complex organic matter in aquatic environments. A novel Gram-reaction-negative, heterotrophic, rod-shaped, aerobic, yellow-pigmented and gliding bacterium, strain SCB36 T , was isolated from a protein-enriched seawater sample, collected at Scripps Pier, Southern California Bight (Eastern Pacific). Analysis of the 16S rRNA gene sequence showed that the bacterium was related to members of the genus Winogradskyella within the family Flavobacteriaceae, phylum Bacteroidetes. 16S rRNA gene sequence similarity to the other Winogradskyella species was 94.5-97.1 %. DNA-DNA relatedness between strain SCB36 T and Winogradskyella thalassocola KMM 3907 T , its closest relative in terms of 16S rRNA gene sequence similarity, was 20 %. On the basis of the phylogenetic and phenotypic data, strain SCB36 T represents a novel species of the genus Winogradskyella, for which the name Winogradskyella rapida sp. nov. is proposed. The type strain is SCB36 T (5CECT 7392 T 5CCUG 56098 T ).Members of the phylum Bacteroidetes make up a significant portion (up to 30 %) of marine bacterioplankton communities (Glöckner et al., 1999). Notably, members of the family Flavobacteriaceae account for a majority of the phylogenetic diversity found among marine Bacteroidetes (Alonso et al., 2007). Members of the phylum Bacteroidetes in general, and members of the family Flavobacteriaceae in particular, have been implicated in the degradation of complex organic matter in the sea (Abell & Bowman, 2005;Kirchman, 2002;Pinhassi et al., 2004). Cottrell & Kirchman (2000) highlighted that marine members of the Bacteroidetes have a preference for the utilization of protein over that of simple monomers. Understanding the taxonomy and the phenotypic characteristics of bacteria that potentially participate in the degradation of organic matter in the sea is an important step in understanding how biogeochemical processes are regulated.Strain SCB36 T was isolated from a seawater mesocosm experiment designed to investigate how a natural bacterioplankton community would respond to protein enrichment (Pinhassi et al., 1999). Enrichment with protein (BSA) was chosen in an attempt to simulate the release of organic matter during the decay phase of a phytoplankton bloom. Surface seawater was collected on 7 December 1995 from Scripps Pier, Southern California Bight (La Jolla, CA, USA; 32 u 539 N 117 u 159 W). The sample was incubated for 3 days at 15 uC with a 12 h dark/12 h light cycle, after which agar plates were prepared for strain isolation as described previously by Pinhassi et al. (1999). Strain SCB36 T was one of the bacteria that exhibited the most pronounced positive growth response to enrichment with protein and reached net growth rates of nearly 1.5 day 21 (see Supplementary Fig. S1, available in IJSEM Online), compared to net growth rates below 0.4 day 21 for the total bacterial community (Pinhassi et al., 1999). Further, its growth rate closely followed the increas...

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confidence: 99%

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confidence: 99%

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Winogradskyella rapida sp. nov., isolated from protein-enriched seawater

Pinhassi¹,

Nedashkovskaya

Hagström³

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…1). Strain WD-2-2 T exhibited 16S rRNA gene sequence similarity of 97.4 % to W.litorisediminis DPS-8 T and 94.5-96.6 % to the type strains of the other species of the genus Winogradskyella.The predominant isoprenoid quinone detected in strain WD-2-2 T was menaquinone-6 (MK-6), in line with other members of the genus Winogradskyella (Nedashkovskaya et al, 2005(Nedashkovskaya et al, , 2012 Begum et al, 2013) and all other members of the family Flavobacteriaceae (Bernardet, 2011). The cellular fatty acid profiles of strain WD-2-2 T and the type strains of W. litorisediminis and W. thalassocola are compared in Table 2.…”

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Winogradskyella wandonensis sp. nov., isolated from a tidal flat

Park

Won

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, non-flagellated, non-gliding, aerobic, rod-shaped bacterium, designated WD-2-2 T , was isolated from a tidal flat of Wando, an island of South Korea, and subjected to a polyphasic taxonomic analysis. Strain WD-2-2 T grew optimally at 30 6C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain WD-2-2 T belonged to the genus Winogradskyella, clustering coherently with the type strain of Winogradskyella litorisediminis. Strain WD-2-2 T exhibited 16S rRNA gene sequence similarity of 97.4 % to W. litorisediminis DPS-8 T and 94.5-96.6 % to the type strains of the other species of the genus Winogradskyella. Strain WD-2-2 T contained MK-6 as the predominant menaquinone and iso-C 15 : 1 G, iso-C 17 : 0 3-OH and iso-C 15 : 0 as the major fatty acids. The major polar lipids detected in strain WD-2-2 T were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C content was 36.4 mol%, and DNA-DNA relatedness with W. litorisediminis DPS-8 T was 13 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain WD-2-2 T is separate from recognized species of the genus Winogradskyella. On the basis of the data presented, strain WD-2-2 T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella wandonensis sp. nov. is proposed. The type strain is WD-2-2 T (5KCTC 32579 T 5CECT 8445 T ).The genus Winogradskyella, a member of family Flavobacteriaceae (phylum Bacteroidetes), was proposed by Nedashkovskaya et al. (2005) with the descriptions of three novel species, Winogradskyella thalassocola (the type species of the genus), W. epiphytica and W. eximia. At the time of writing, the genus comprised 17 species with validly published names (http://www.bacterio.net/winogradskyella.html; Euzéby, 1997). All known members of the genus Winogradskyella have been isolated from marine environments or marine organisms (Lau et al., 2005;Nedashkovskaya et al., 2005Nedashkovskaya et al., , 2009Nedashkovskaya et al., , 2012Pinhassi et al., 2009;Romanenko et al., 2009;Ivanova et al., 2010;Kim & Nedashkovskaya, 2010;Yoon et al., 2011;Lee et al., 2012; Begum et al., 2013;Lee et al., 2013). In this study, we describe a Winogradskyella-like bacterial strain, designated WD-2-2 T , collected from a tidal flat sediment. The aim of the present work was to determine the exact taxonomic position of strain WD-2-2 T by polyphasic taxonomic characterization which included determination of chemotaxonomic and other phenotypic properties, detailed phylogenetic investigations based on 16S rRNA gene sequences and DNA-DNA hybridization.A sample of tidal flat sediment was collected from Wando, an island of South Korea, on the South Sea, and used as the source for isolation of bacterial strains. Strain WD-2-2 T was isolated by the standard dilution plating technique at 25 u C on marine agar 2216 (MA; Becton Dickinson) and cultivate...

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“…Colonies on MA are circular, slightly raised, smooth, yellow to pale-yellow in colour and 2?0-3?0 mm in diameter after incubation for 2 days at 30 u C. Growth occurs at 7-42 u C, with an optimum at 30 u C; growth does not occur at 4 or 45 uC. Growth is observed at pH 6?0-8?0, with an optimum at pH 7?2-7?4; growth does (Vandamme et al, 1999); 3, Gaetbulimicrobium (Yoon et al, 2006a); 4, Cellulophaga (Johansen et al, 1999;Bowman, 2000;Nedashkovskaya et al, 2004b); 5, Stanierella (Reichenbach, 1989;Nedashkovskaya et al, 2005b); 6, Winogradskyella (Nedashkovskaya et al, 2005a;Lau et al, 2005); 7, Psychroflexus (Bowman et al, 1998;Donachie et al, 2004). All are positive for alkaline phosphatase (not determined for Stanierella) and catalase activities.…”

Section: Description Of Zhouia Amylolytica Sp Novmentioning

confidence: 99%

Zhouia amylolytica gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from sediment of the South China Sea

Liu

Wang

Dai

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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Three Gram-negative, non-spore-forming strains were isolated from sediment from the South China Sea, China, and their taxonomic positions were investigated using a polyphasic approach. Strains HN-171T, HN-172 and HN-181 grew optimally at 30 °C, in the presence of 4.5–5.0 NaCl % (w/v) and at pH 7.2–7.4. They contained MK-6 as the predominant respiratory quinone and contained iso-C15 : 1 G, iso-C15 : 0, summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c/t) and C15 : 0 as the major fatty acids. The DNA G+C content of strain HN-171T was 34.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that strain HN-171T, together with strains HN-172 and HN-181, formed a distinct evolutionary lineage within the family Flavobacteriaceae. The 16S rRNA gene sequences of strains HN-171T, HN-172 and HN-181 shared 99.8–100 % similarity with each other, and the sequence of strain HN-171T exhibited similarity values below 90.2 % with those of other members of the family Flavobacteriaceae. The closest relative of HN-171T was Coenonia anatina LMG 14382T (90.2 %). On the basis of their phenotypic and phylogenetic properties, the three isolates represent a novel genus and a novel species, for which the name Zhouia amylolytica gen. nov., sp. nov. is proposed. The type strain is HN-171T (=CGMCC 1.6114T=JCM 14016T).

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Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae

Cited by 99 publications

References 21 publications

Winogradskyella rapida sp. nov., isolated from protein-enriched seawater

Winogradskyella rapida sp. nov., isolated from protein-enriched seawater

Winogradskyella wandonensis sp. nov., isolated from a tidal flat

Zhouia amylolytica gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from sediment of the South China Sea

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