Winogradskyella pulchriflava sp. nov., isolated from marine sediment

Abstract

A taxonomic study was conducted on strain EM106 T , isolated from a sediment sample of the East Sea, Republic of Korea. Comparative 16S rRNA gene sequence analyses showed that strain EM106 T belongs to the family Flavobacteriaceae and is most closely related to Winogradskyella echinorum KMM 6211 T and Winogradskyella ulvae KMM 6390 T (97.8 and 97.3 % 16S rRNA gene sequence similarities, respectively). The G+C content of the genomic DNA of strain EM106 T was 33.3 mol%, and the major respiratory quinone was menaquinone-6. The polar lipids of EM106 T were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. DNA-DNA relatedness data indicated that strain EM106 T represented a distinct species, separate from W. echinorum KMM 6211 T and W. ulvae KMM 6390 T . Strain EM106 T possessed iso-C 15 : 0 , iso-C 15 : 1 G and iso-C 16 : 0 3-OH as the major cellular fatty acids. The isolate was Gram-staining-negative, strictly aerobic, short rod-shaped and motile by gliding. The strain grew at 10-35 6C (optimum, 25 6C), pH 6.5-9.0 (optimum, 7.5), and with 0.5-5 % (w/v) NaCl (optimum, 0.5-1 % NaCl). The overall physiological features of strain EM106 T were very similar to those of W. echinorum KMM 6211 T but only strain EM106 T had nitrate reductase activity. On the basis of phenotypic and phylogenetic analyses, strain EM106 T is proposed to represent a novel species, Winogradskyella pulchriflava. The type strain is EM106 T (5KCTC 23858 T 5NCAIM B 02481 T ).The genus Winogradskyella, within the family Flavobacteriaceae, was first described by Nedashkovskaya et al. (2005) to accommodate Gram-staining-negative, heterotrophic, strictly aerobic, rod-shaped bacteria (without production of flexirubin-type pigments) isolated from the sea urchin Strongylocentrotus intermedius. At the time of writing, the genus comprises 15 recognized

Winogradskyella sediminis sp. nov., isolated from marine sediment

“…The genomic DNA G +C content of strain S5-23-3 T was 36.1 mol%, which is within the range reported for the type strains of other Winogradskyella species (Nedashkovskaya et al, 2012;Kang et al, 2013;Kim et al, 2013Kim et al, , 2015Park & Yoon 2013 (Nedashkovskaya et al, 2005(Nedashkovskaya et al, , 2012Pinhassi et al, 2009;Kang et al, 2013;Park & Yoon 2013;Kim et al, 2015).…”

Winogradskyella eckloniae sp. nov., a marine bacterium isolated from the brown alga Ecklonia cava

“… * Different results were reported by Kim et al (2013) and Nedashkovskaya et al (2009). † DNA G+C content data from: a , Kim et al (2013); b , Nedashkovskaya et al (2009); c , Nedashkovskaya et al (2012). …”

“…At the time of writing, the genus comprised 20 species with validly published names (http://www.bacterio.net/). Members of the genus Winogradskyella have been isolated from various marine environments, such as marine animals (Lau et al , 2005; Nedashkovskaya et al , 2009; Ivanova et al , 2010), marine sediments (Romanenko et al , 2009; Begum et al , 2013; Kang et al , 2013; Kim et al , 2013; Park et al , 2014), seawater (Pinhassi et al , 2009; Kim & Nedashkovskaya, 2010; Yoon et al , 2011; Yoon & Lee, 2012; Lee et al , 2012, 2013) and seaweeds (Nedashkovskaya et al , 2005, 2012; Park & Yoon, 2013).…”

“… † DNA G+C content data from: a , Kim et al (2013); b , Nedashkovskaya et al (2009); c , Nedashkovskaya et al (2012). …”

Winogradskyella crassostreae sp. nov., isolated from an oyster (Crassostrea gigas)

“…The nucleotides were eluted by a mixture of 0.55 M NH 4 H 2 PO 4 (pH 4.0) and acetonitrile (40[hairsp] :[hairsp] 1, v/v), using a flow rate of 1 ml min -1 at room temperature and detected by UV absorbance at 270 nm. The DNA G+C content of strain TYO-19 T was 39.0 mol%, a value higher than those of the type strains of W. epiphytica and other species of the genus Winogradskyella (; Kim & Oh, 2012; Yoon & Lee, 2012; Begum et al , 2013; Kang et al , 2013; Kim et al , 2013; Park & Yoon, 2013; Park et al , 2014).…”