1983
DOI: 10.1021/jf00118a063
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Winged bean (Psophocarpus tetragonolobus) tannin level, phytate content and hemagglutinating activity

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Cited by 60 publications
(26 citation statements)
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“…In contrast, there are several contaminants still present in winged bean namely; behenic acid, parinaric acid, trypsin inhibitors, phytic acid, tannin and lectin (de Lumen and Chan, 1986;Fernando and Bean, 1986;Kotaru et al, 1987). Yet, the trypsin inhibitors present in the winged bean are heat tolerant and require alternative methods for treatment like fermentation (Tan et al, 1983(Tan et al, , 1984a(Tan et al, , 1984b. Moreover, these anti-nutritional factors differ depending on different cultivars making it feasible to select beans with a lower content of these antinutritional factors (Fernando and Bean, 1985).…”
Section: Winged Beanmentioning
confidence: 99%
“…In contrast, there are several contaminants still present in winged bean namely; behenic acid, parinaric acid, trypsin inhibitors, phytic acid, tannin and lectin (de Lumen and Chan, 1986;Fernando and Bean, 1986;Kotaru et al, 1987). Yet, the trypsin inhibitors present in the winged bean are heat tolerant and require alternative methods for treatment like fermentation (Tan et al, 1983(Tan et al, , 1984a(Tan et al, , 1984b. Moreover, these anti-nutritional factors differ depending on different cultivars making it feasible to select beans with a lower content of these antinutritional factors (Fernando and Bean, 1985).…”
Section: Winged Beanmentioning
confidence: 99%
“…The analysis for lectin content was conducted by the haemagglutination assay method described by Gordon and Marquardt [32] in the presence of 10 mM Mn 2+ in roundbottomed wells of microtitre plates using 2% (v/v) trypsinized cattle blood erythrocytes suspension in saline phosphate buffer, pH 7.0 [28]. The lectin activity against 3% trypsinized type O human erythrocytes was determined as described by Tan et al [33]. The haemagglutination activity was defined as the minimum amount of the bean material in mg per ml of the assay medium which produced agglutination.…”
Section: Analysis Of Various Antinutritional Componentsmentioning
confidence: 99%
“…This is generally accomplished by heating or physical separation of legume components as described by Bishnoi and Khetarpaul (1993), Borowska and Kozlowska (1986), Deshpande et al (1982), Kantha et al (1986), andVidal-Valverde et al (1994). Tannin or polyphenol content is generally greater in pigmented legumes and hemagglutinating activity is altered by heating (Tan et al, 1983). Phytic acid (inositol hexakisphosphate, IP 6 ), the subject of this communication, is considered an antinutrient by virtue of its potential adverse action on the utilization of dietary cations (Morris, 1986).…”
mentioning
confidence: 99%