Wine polyphenols modulate calcium handling in rat aorta: involvement of nitric oxide pathway

Abstract

The effects of short-term oral administration of red wine polyphenolic compounds (RWPCs) on blood pressure and vascular reactivity were investigated in rats. The consequence of RWPCs treatment on agonist-induced contractility of rat aorta with respect to Ca2+ handling was assessed, by examining both intracellular Ca2+ store and extracellular Ca2+ influx components of the response. Rats were treated daily for 7 days by intragastric administration of either 5% glucose, or RWPCs (20 mg/kg) [from two different sources, i.e. Provinols (SFD, Vallont Pont d'Arc, France) and RWPC1 (INRA, Montpellier, France)]. Administration of these compounds produced a decrease in systolic blood pressure. The consequence of RWPCs treatment on vascular smooth muscle was investigated in rat aorta without endothelium exposed to noradrenaline. In Ca(2+)-free medium, RWPC1 but not Provinols treatment induced an increase in noradrenaline-induced contraction. After depletion of intracellular Ca2+ stores by noradrenaline in Ca(2+)-free medium, addition of CaCl2 in the continuous presence of agonist induced an increase in contraction, which was not significantly different between control, Provinols- and RWPC-treated rats. The presence of an inhibitor of sarcoplasmic reticulum Ca(2+)-ATPase, thapsigargin, significantly reduced noradrenaline-induced contraction in Ca(2+)-free medium in RWPCs-treated aorta, as compared to that of control. Interestingly, the inhibitory effect of thapsigargin on the response linked to the release of Ca2+ from internal stores in RWPCs-treated vessels was completely prevented in the presence of NO-synthase inhibitor, L-nitro arginine methyl ester, the inhibitor of guanylyl cyclase, oxadiazolo-quinoxaline or the protein kinase G inhibitor, 8-Bromoguanosine-3'-5-cyclic mono-phosphorothioate, Rp isomer. These results suggest that short-term administration of RWPCs in rats induced subtle alteration of thapsigargin-sensitive component of agonist-induced contraction in rat aorta linked to Ca2+ release from intracellular store. Calcium release from intracellular stores sensitive to thapsigargin was implicated in this mechanism. The prevention of the inhibitory effect of thapsigargin by the inhibitors of NO/cyclic guanosine monophosphate pathway after RWPCs treatment highlights the role of NO in this phenomenon.

Flavanones protect from arterial stiffness in postmenopausal women consuming grapefruit juice for 6 mo: a randomized, controlled, crossover trial

“…The present study does not identify the nature or origin of the changes in arterial stiffness induced by grapefruit flavanones. However, previous studies using different categories of flavonoids, including soy isoflavones and wine flavanols, have demonstrated their ability to modulate calcium handling in human and rat arteries (38,39). This effect is consistent with clinical data showing the action of flavonoids on arterial stiffness because this action is directly related to vascular tone, which is itself dependent on intracellular free calcium.…”

Mangifera indica L. extract protects T cells from activation-induced cell death

“…This effect could be related with previous reports that mangiferin inhibits crel and Rel-A which interact with intracellular Ca 2+ -loaded calmodulin [36]. Pholyphenols also show a strong capacity to modulate Ca 2+ levels [37].…”

Effect of oil palm phenolics on gastrointestinal transit, contractility and motility in the rat

“…For example, recent studies have identified a range of beneficial biological outcomes of oil palm phenolics (OPP) including antioxidant, antiinflammatory, anti-diabetic, neuro-protective as well as vascular protective qualities (Abeywardena et al, 2002;Bolsinger, Pronczuk, Sambanthamurthi, & Hayes, 2014;Leow, Sekaran, Sundram, Tan, & Sambanthamurthi, 2013a;Leow, Sekaran, Tan, Sundram, & Sambanthamurthi, 2013b;Nasef, Mehta, Murray, Marlow, & Ferguson, 2014;Sambanthamurthi et al, 2011). These observation for OPP are in agreement with several other plant extracts and isolated polyphenols and flavonoids reported to exert biological actions on various cardiac (Fang et al, 2011;Wallace, Baczko, Jones, Fercho, & Light, 2006;Wu, Loh, Cheng, Lu, & Lin, 2013) and vascular smooth muscle cell preparations including modulation of contractility (Diebolt & Andriantsitohaina, 2002;Protic et al, 2013;Zhu, Wang, Ou, Han, & Li, 2013). Several polyphenols exert their effects on contractility usually by modulation of ion channels (Protic et al, 2013;Tadano et al, 2010;Wu et al, 2013) and other important pathways linked to motility in health and disease (Abe et al, 2012).…”

“…It has been further demonstrated that forms of dietary resistant starch (RS), that result in the microbial generation of short chain fatty acids (SCFA), can modulate the contractility of the proximal colon (Bajka et al, 2010;Patten et al, 2015) and increase motility of the distal colon (Soret et al, 2010). Since plant polyphenols may also affect smooth muscle contractility (Diebolt & Andriantsitohaina, 2002), the aim of this study was to examine the effects of OPP in the diet on whole gut transit, colonic contractility and distal colonic motility of the rat compared to a source of RS, high amylose maize starch (HAMS), that like polyphenols, is resistant to upper gut digestion. Our hypothesis is that OPP will influence gut contractility and motility.…”