Wilt disease of castor: an overview

Prasad, M. Santha Lakshmi; Raoof, M. A.; Gayatri, B.; Anjani, K.; Lavanya, C.; Prasad, R. D.; Senthilvel, Senapathy

doi:10.1007/s42360-019-00144-7

Cited by 14 publications

(11 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, castor fusarium wilt being a soil and seed borne pathogen (Prasad et al, 2019;Dange, 2003), colonizes the plant xylem vessels and block them completely causing plant wilting (Dange, 2003). Generally, for the control of this disease fungicides are used (Maitlo et al, 2014).…”

Section: Issn: 2319-7706 Volume 10 Number 05 (2021)mentioning

confidence: 99%

Screening the Efficacy of different Fungicides against Fusarium oxysporum f. sp. ricini in Castor

Bokka¹,

Palaniappan²

2021

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Section: Issn: 2319-7706 Volume 10 Number 05 (2021)mentioning

confidence: 99%

Screening the Efficacy of different Fungicides against Fusarium oxysporum f. sp. ricini in Castor

Bokka¹,

Palaniappan²

2021

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…Buchw.) Hennebert (Prasad et al, 2016). The anamorphic phase is mainly responsible for causing disease epiphytotics.…”

Section: Introductionmentioning

confidence: 99%

Screening germplasm lines for identification of resistant source against gray mold disease (Amphobotrys ricini (N.F. Buchw.) Hennebert) of castor

Greeshma

Devi

Prasad

et al. 2023

ECJ

View full text Add to dashboard Cite

The present study was aimed to screen castor germplasm lines varying in wax content against gray mold disease for the identification of resistance source under in vitro conditions using detached spike and detached capsule technique. Out of thirty-three lines screened against gray mold under in vitro conditions six lines with low wax content viz., RG-1754 (0.24 µg/mg), RG-1875 (0.12 µg/mg), RG-1915 (0.21 µg/mg), RG-1919 (0.24 µg/mg), RG-1972 (0.08 µg/mg) and RG-1926 (0.08 µg/mg) recorded significantly low levels of infection ranging from 10 to 20 per cent 7 days after inoculation. Whereas the susceptible cultivar DCH -519 has recorded disease severity of more than 90 %. The pearman's rank correlation analysis showed a strong positive relation between disease severity and capsule wax content, with p < 0.01 and r = 0.884.

show abstract

“…Wilt disease caused by Fusarium oxysporum f. sp. ricini is an important oil and seed borne disease in castor and results in significant yield losses [ 16 ]. Thus, studies of phloem mobility of vectorized agrochemicals contribute to the control of castor wilt disease by foliar spraying.…”

Section: Introductionmentioning

confidence: 99%

An efficient overexpression method for studying genes in Ricinus that transport vectorized agrochemicals

Xiao

Zhang

et al. 2022

Plant Methods

View full text Add to dashboard Cite

Background Plant plasma membrane transporters play essential roles during the translocation of vectorized agrochemicals. Therefore, transporters associated with phloem loading of vectorized agrochemicals have drawn increasing attention. As a model system, castor bean (Ricinus communis L.) has been widely used to detect the phloem mobility of agrochemicals. However, there is still a lack of an efficient protocol for the Ricinus seedling model system that can be directly used to investigate the recognition and phloem loading functions of plasmalemma transporters toward vectorized agrochemicals. Results Here, using vacuum infiltration strategy, we overexpressed the coding gene for enhanced green fluorescent protein (eGFP) in R. communis seedlings by Agrobacterium tumefaciens-mediated transformation system. Strong fluorescence signals were observed in leaves, demonstrating that exogenous genes can be successfully overexpressed in seedlings. Subsequently, gene expression time and vacuum infiltration parameters were optimized. Observation of fluorescence and qRT-PCR analysis showed that eGFP strength and expression level reached a peak at 72 h after overexpression in seedlings. Parameter optimization showed Agrobacterium concentration at OD600 = 1.2, and infiltration for 20 min (0.09 MPa), return to atmospheric pressure, and then infiltration for another 20 min, were the suitable transformation conditions. To test the application of vacuum agroinfiltration in directly examining the loading functions of plasma membrane transporters to vectorized agrochemicals in seedlings, two LHT (lysine/histidine transporter) genes, RcLHT1 and RcLHT7, were overexpressed. Subcellular localization showed the strong fluorescent signals of the fusion proteins RcLHT1-eGFP and RcLHT7-eGFP were observed on the cell membrane of mesophyll cells, and their relative expression levels determined by qRT-PCR were up-regulated 47- and 52-fold, respectively. Furthermore, the concentrations of l-Val-PCA (l-valine-phenazine-1-carboxylic acid conjugate) in phloem sap collected from seedling sieve tubes were significantly increased 1.9- and 2.3-fold after overexpression of RcLHT1 and RcLHT7, respectively, implying their roles in recognition and phloem loading of l-Val-PCA. Conclusions We successfully constructed a transient expression system in Ricinus seedlings and laid the foundation for researchers to directly investigate the loading functions of plasma membrane transporters to vectorized agrochemicals in the Ricinus system.

show abstract

Wilt disease of castor: an overview

Cited by 14 publications

References 8 publications

Screening the Efficacy of different Fungicides against Fusarium oxysporum f. sp. ricini in Castor

Screening the Efficacy of different Fungicides against Fusarium oxysporum f. sp. ricini in Castor

Screening germplasm lines for identification of resistant source against gray mold disease (Amphobotrys ricini (N.F. Buchw.) Hennebert) of castor

An efficient overexpression method for studying genes in Ricinus that transport vectorized agrochemicals

Contact Info

Product

Resources

About