Wilt and Root Rot Complex of Important Pulse Crops: Their Detection and Integrated Management

Kumari, Nidhi; Katoch, Shabnam

doi:10.1007/978-3-030-35947-8_6

Cited by 14 publications

(8 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sustainable production of pea has been negatively affected by several diseases, predominantly root rots (Akhtar and Azam, 2014;Bodah et al, 2016). Root rots are the diseases of greatest impact to crop production worldwide (Kumari and Katoch, 2020). Frequently, root rot diseases involve more than one pathogen; therefore, the disease is known as root rot complex.…”

Section: Introductionmentioning

confidence: 99%

Identification of Fusarium solani f. sp. pisi (Fsp) Responsive Genes in Pisum sativum

et al. 2020

View full text Add to dashboard Cite

Pisum sativum (pea) is rapidly emerging as an inexpensive and significant contributor to the plant-derived protein market. Due to its nitrogen-fixation capability, short life cycle, and low water usage, pea is a useful cover-and-break crop that requires minimal external inputs. It is critical for sustainable agriculture and indispensable for future food security. Root rot in pea, caused by the fungal pathogen Fusarium solani f. sp. pisi (Fsp), can result in a 15-60% reduction in yield. It is urgent to understand the molecular basis of Fsp interaction in pea to develop root rot tolerant cultivars. A complementary genetics and gene expression approach was undertaken in this study to identify Fsp-responsive genes in four tolerant and four susceptible pea genotypes. Time course RNAseq was performed on both sets of genotypes after the Fsp challenge. Analysis of the transcriptome data resulted in the identification of 42,905 differentially expressed contigs (DECs). Interestingly, the vast majority of DECs were overexpressed in the susceptible genotypes at all sampling time points, rather than in the tolerant genotypes. Gene expression and GO enrichment analyses revealed genes coding for receptor-mediated endocytosis, sugar transporters, salicylic acid synthesis, and signaling, and cell death were overexpressed in the susceptible genotypes. In the tolerant genotypes, genes involved in exocytosis, and secretion by cell, the anthocyanin synthesis pathway, as well as the DRR230 gene, a pathogenesis-related (PR) gene, were overexpressed. The complementary genetic and RNAseq approach has yielded a set of potential genes that could be targeted for improved tolerance against root rot in P. sativum. Fsp challenge produced a futile transcriptomic response in the susceptible genotypes. This type of response is hypothesized to be related to the speed at which the pathogen infestation advances in the susceptible genotypes and the preexisting level of disease-preparedness in the tolerant genotypes.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of Fusarium solani f. sp. pisi (Fsp) Responsive Genes in Pisum sativum

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The profitable production of pea is threatened by soilborne diseases. These diseases are commonly referred to as the pea root rot complex (PRRC) and are caused by a single or combination of pathogens, including Aphanomyces euteiches, Fusarium spp., Mycosphaerella pinodes, Pythium spp., and Rhizoctonia solani (Xue, 2003;Kumari and Katoch, 2020). One of the predominant causal agents of PRRC is the fungus Fusarium solani f. sp.…”

Section: Introductionmentioning

confidence: 99%

Identification of Root Rot Resistance QTLs in Pea Using Fusarium solani f. sp. pisi-Responsive Differentially Expressed Genes

et al. 2021

View full text Add to dashboard Cite

Pisum sativum (pea) yields in the United States have declined significantly over the last decades, predominantly due to susceptibility to root rot diseases. One of the main causal agents of root rot is the fungus Fusarium solani f. sp. pisi (Fsp), leading to yield losses ranging from 15 to 60%. Determining and subsequently incorporating the genetic basis for resistance in new cultivars offers one of the best solutions to control this pathogen; however, no green-seeded pea cultivars with complete resistance to Fsp have been identified. To date, only partial levels of resistance to Fsp has been identified among pea genotypes. SNPs mined from Fsp-responsive differentially expressed genes (DEGs) identified in a preceding study were utilized to identify QTLs associated with Fsp resistance using composite interval mapping in two recombinant inbred line (RIL) populations segregating for partial root rot resistance. A total of 769 DEGs with single nucleotide polymorphisms (SNPs) were identified, and the putative SNPs were evaluated for being polymorphic across four partially resistant and four susceptible P. sativum genotypes. The SNPs with validated polymorphisms were used to screen two RIL populations using two phenotypic criteria: root disease severity and plant height. One QTL, WB.Fsp-Ps 5.1 that mapped to chromosome 5 explained 14.8% of the variance with a confidence interval of 10.4 cM. The other four QTLs located on chromosomes 2, 3, and 5, explained 5.3–8.1% of the variance. The use of SNPs derived from Fsp-responsive DEGs for QTL mapping proved to be an efficient way to identify molecular markers associated with Fsp resistance in pea. These QTLs are potential candidates for marker-assisted selection and gene pyramiding to obtain high levels of partial resistance in pea cultivars to combat root rot caused by Fsp.

show abstract

“…Root rots have a significant impact on global crop production [1]. Depending on the causal agent, host susceptibility, and the environmental conditions, crop losses can range from slightly above the economic threshold to losing complete fields.…”

Section: Introductionmentioning

confidence: 99%

“…If symptoms appear aboveground, the plants usually fail to recover. Some of the symptoms associated with root rots include browning and softening of root tips, root lesions that vary in size and color (reddish, brown, and black), yellowing and wilting of leaves, stunted plant growth, reduced yield, and loss of crop [1,3,[16][17][18][19]. Selected root rot pathogens can also cause post-harvest rots in beets, potato, and sweet potato.…”

Section: Introductionmentioning

confidence: 99%

“…Selected root rot pathogens can also cause post-harvest rots in beets, potato, and sweet potato. The proliferation of root rot pathogens is favored by moderate to high soil moisture, poor drainage conditions, soil compaction, the optimal temperature for pathogen growth, mono-cropping, and other factors that contribute to plant stress [1,[20][21][22]. The unpredictable climatic conditions portend an increase in mean temperatures and other natural calamities such as droughts, floods, and storms.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Understanding Root Rot Disease in Agricultural Crops

Williamson-Benavides¹,

Dhingra²

2020

Preprint

View full text Add to dashboard Cite

Root rot diseases remain a major global threat to the productivity of agricultural crops. They are usually caused by more than one type of pathogen and are thus often referred to as a root rot complex. Fungal and oomycete species are the predominant participants in the complex, while bacteria and viruses are also known to cause root rot. Incorporating genetic resistance in cultivated crops is considered as the most efficient and sustainable solution to counter root rot; however, resistance is often quantitative in nature. Several genetics studies in various crops have identified quantitative trait loci associated with resistance. With access to whole genome sequences, the identity of the genes within the reported loci is becoming available. Several of the identified genes have been implicated in pathogen response. However, it is becoming apparent that at the molecular level, each pathogen engages a unique set of proteins to either infest the host successfully or be defeated or contained in doing so. In this review, a comprehensive summary of the genes and potential mechanisms underlying resistance or susceptibility against the most investigated root rots of important agricultural crops is presented.

show abstract

Wilt and Root Rot Complex of Important Pulse Crops: Their Detection and Integrated Management

Cited by 14 publications

References 86 publications

Identification of Fusarium solani f. sp. pisi (Fsp) Responsive Genes in Pisum sativum

Identification of Fusarium solani f. sp. pisi (Fsp) Responsive Genes in Pisum sativum

Identification of Root Rot Resistance QTLs in Pea Using Fusarium solani f. sp. pisi-Responsive Differentially Expressed Genes

Understanding Root Rot Disease in Agricultural Crops

Contact Info

Product

Resources

About