The effect of land application of biomaterials based on two strains ofTrichodermafungus on phytoremediation processes was studied. Six metals (Cd, Cr, Cu, Pb, Zn, and Ni) were analysed in soil and soil leachate as well as in plant tissues. The translocation index (Ti) and metal bioconcentration factors (BCF) calculated for the inoculated plants were increased compared to the noninoculated control, except for Pb andSalixsp. Simultaneously, the mobilisation of metals in soil solution as an effect of biomaterials was noted. The highest values ofTi—339% (for Cr), 190% (for Ni), and 110% (for Cu)—were achieved for the combinationMiscanteus giganteusandTrichodermaMSO1. The results indicated that the application of fungus has positive effects on increasing the biomass, soil parameters (C, N, and P), and solubility of heavy metals in soil and therefore in enhancing phytoextraction forMiscanthus giganteusL.,Panicum virgatumL.,Phalaris arundinaceaL., andSalixsp.